个人简介
2012.09–至今 江南大学医学院，教授，博导
2008.06–2012.09 江南大学医药学院，教授，博导
2002.06–2008.06 江南大学医学院，副教授
1990.03–2000.10江苏无锡启蒙生物高技术研究所，高级工程师，所长
1997.09–2001.03 复旦大学，分子生物学，博士
1987.09–1990.07 江南大学，粮油工程，硕士
1979.09–1983.07 东南大学，力学，学士
近期论文
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1. C Li, BC Hu, D Hu, XF Xu, XC Zong, JP Li, MC Wu*. Stereoselective ring-opening of styrene oxide at elevated concentration byPhaseolus vulgarisepoxide hydrolase,PvEH2, in the organic/aqueous biphasic system.Catalysis communications, 2019, 123: 1–5.
2. C Li, J Zhao, D Hu, BC Hu, R Wang,MC Wu*, J Zang. Multiple site-directed mutagenesis of aPhaseolus vulgaris epoxide hydrolase to improve its catalytic performance towardsp-chlorostyrene oxide based on the computer-aided re-design.International Journal of Biological Macromolecules, 2019, 121: 326–332.
3. JF Li, XQ Li, Y Liu, FJ Yuan, T Zhang,MC Wu*, JR Zhang. Directed modification of l-LcLDH1, an l-lactate dehydrogenase fromLactobacillus casei, to improve its specific activity and catalytic efficiency towards phenylpyruvic acid.Journal of Biotechnology, 2018, 281: 193–198.
4. BC Hu, C Li, R Wang, XC Zong, JP Li, JF Li,MC Wu*, Improvement in the activity and enantioconvergency ofPvEH3, an epoxide hydrolase fromPhaseolus vulgaris, forp-chlorostyrene oxide by sitesaturation mutagenesis,Catalysis communications, 2018, 117: 9–13.
5. D Hu, CD Tang, C Li, TT Kan, XL Shi, L Feng,MC Wu*. Stereoselective hydrolysis of epoxides by reVrEH3, a novelvigna radiataepoxide hydrolase with high enantioselectivity or high and complementary regioselectivity.Journal of Agricultural and Food Chemistry, 2017, 65(45): 9861–9870.
6. D Hu, R Wang, XL Shi, HH Ye, Q Wu,MC Wu*, JJ Chu. Kinetic resolution of racemic styrene oxide at a high concentration by recombinantAspergillus usamiiepoxide hydrolase in an n-hexanol/buffer biphasic system.Journal of Biotechnology, 2016, 236: 152–158.
7. YH Dong, JF Li, D Hu, X Yin, CJ Wang, SH Tang, MC Wu*. Replacing a piece of loop-structure in the substrate-binding groove ofAspergillus usamiiβ-mannanase, AuMan5A, to improve its enzymatic properties by rational design.Applied Microbiology and Biotechnology, 2016, 100(9): 3989–3998.
8. D Hu, CD Tang, B Yang, JC Liu, T Yu, C Deng,MC Wu*. Expression of a novel epoxide hydrolase ofAspergillus usamiiE001 inEscherichia coli and its performance in resolution of racemic styrene oxide.Journal of Industrial Microbiology & Biotechnology, 2015, 42(5): 671–680.
9. X Yin, JF Li JF, CJ Wang, D Hu, Q Wu, Y Gu,MC Wu*. Improvement in the thermostability of a type A feruloyl esterase, AuFaeA, fromAspergillus usamiiby iterative saturation mutagenesis.Applied Microbiology and Biotechnology, 2015, 99(23): 10047–10056.
10. HM Zhang, JF Li, JQ Wang, YJ Yang,MC Wu*. Determinants for the improved thermostability of a mesophilic family 11 xylanase predicted by computational methods.Biotechnology for Biofuels, 2014, 7(1):3.
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