Li, Lingjun - University of Wisconsin-Madison

个人简介

Lingjun received her BE degree in Environmental Analytical Chemistry from Beijing Polytechnic University and a PhD degree in Analytical Chemistry/Biomolecular Chemistry from University of Illinois at Urbana-Champaign. She did three-way postdoctoral research at the Pacific Northwest National Laboratory, Brandeis University, and University of Illinois before joining the School of Pharmacy faculty in 2002. She currently holds joint appointments in the School of Pharmacy and Department of Chemistry at UW-Madison, as well as being named Charles Melbourne Johnson Distinguished Chair and Vilas Distinguished Achievement Professor. Ph.D. University of Illinois at Urbana-Champaign, 1995-2000 May 2000 Chemistry major (analytical and biomolecular) B.E. Beijing University of Technology, Beijing, China, 1987-1992 July 1992 Chemistry major (environmental analytical chemistry)

研究领域

Quantitative MS Strategies We have validated several strategies for quantitative mass spectrometry studies. Among these are DiPyro, DiLeu, and SUGAR. These strategies are regularly employed for proteome profiling, neuropeptide analysis, and post-translational modification quantitation. Comparative Neuropeptide Study Neuropeptide changes induced by food intake, environmental stress, etc. in crustacean models are profiled and compared in this research area. Along with tissue biopsy strategies, microdyalisis is employed to monitor neuropeptide secretion in live animals. Biomarker Discovery Neuropeptide changes induced by food intake, environmental stress, etc. in crustacean models are profiled and compared in this research area. Along with tissue biopsy strategies, microdyalisis is employed to monitor neuropeptide secretion in live animals. Mass Spectrometry Imaging Mass spectrometric imaging enables scientists to obtain 2D and 3D maps of biological analytes in their native tissues without the need for antibodies. The Li Research group implements this technology to examine distribution patterns of neuropeptides, proteins, lipids, and metabolites in a variety of tissues. Ion Mobility-Mass Spectrometry Ion Mobility - Mass Spectrometry separates gas-phase ions by mass, charge, size, and shape through collisions with inert gas molecules. The Li Research Group is currently utilizing IM-MS to develop novel strategies for glycan separation, peptide identification, and structural analyses. Capillary Electrophoresis MS Capillary zone electrophoresis and capillary isoelectric focusing are being developed to couple with MS detection and MS imaging, both off-line and on-line, for enhanced neuropeptidomic and glycomic analysis.

近期论文

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L. Li*, P.E. Andren, and J.V. Sweedler (2018). Editorial and Review: 29th ASMS Sanibel Conference on Mass Spectrometry-Peptidomics: Bridging the Gap between Proteomics and Metabolomics by MS. Journal of American Society for Mass Spectrometry 29, 801-806. doi: 10.1007/s13361-018-1939-5. S. D. Sackett, D.M. Tremmel, F. Ma, A.K. Feeney, R.M. Maguire, M.E. Brown, Y. Zhou, X. Li, C. O’Brien, L. Li, W.J. Burlingham, and J.S. Odorico (2018). Extracellular matrix scaffold and hydrogel derived from decellularized and delipidized human pancreas. Scientific Reports. 8:10452 |DOI:10.1038/s41598-018-28857-1. Published online July 11, 2018. A.R. Buchberger, K. DeLaney, Y. Liu, K. Helfenbein, N. Vu, and L. Li* (2018). Mass spectrometric Profiling of neuropeptides in Callinectes sapidus during hypoxia stress. ACS Chemical Neuroscience. To Be submitted. B. Chen, R.W. Cheloha, N. Kumar, M. Xu, J.W. Checco, S.T. Nelson, R.G. Thorne, S.H. Gellman, and L.Li* (2018). Stability analysis of parathyroid hormone (1-34) and its bioactive analog M-Cys-9 in rat kidney homogenate by MALDI-MS and multiplexed parallel reaction monitoring LC-MS/MS. ACS Chemical Biology To be submitted. C.A. Turski, G.N. Turski, B. Chen, H. Wang, M. Heidari, L. Li, K.K. Noguchi, C. Westmark, I. Duncan, and C. Ikonomidou* (2018). Clemastine effects in rat models of a myelination disorder. Pediatric Research. 83, 1200-1206. Y. Liu, A. R. Buchberger, K. DeLaney, Z. Li and L. Li* (2018). Multifaceted mass spectrometric Investigation of neuropeptide changes in blue crab, Callinectes sapidus, in response to ocean acidification.Journal of Proteome Research. To be submitted. A. Herbst, D. Gushue, R. Cunningham, J. Wang, S. Zafar, J. Safar, I. Zerr, D. McKenzie, L. Li, and J. M. aiken* (2018). Cerebrospinal fluid ribonuclease T2 abundance is a mechanistic biomarker of disease Progression in Creutzfeldt-Jakob and Alzheimer’s disease. Journal of Clinical Investigation. Submitted. L. Hao, Y. Shi, S. Thomas, C.M. Vezina, S. Bajpai, A. Ashok, C. Bieberich, W. A. Ricke, and L. Li*(2018). Comprehensive urinary metabolomics characterization of a genetically induced mouse model of Prostatic inflammation. International Journal of Mass Spectrometry. Invited contribution to the Special Issue for Dr. Shuying Liu’s 75th Birthday. In press. J. Bratburd, C. Keller, E. Vivas, E. Gemperline, L. Li*, F. E. Rey*, and C.R. Currie* (2018). Gut Microbial and metabolic responses to Salmonella enterica Tymphimurium and Candida albicans. mBio, accepted. Y. Feng, B. Chen, D. Frost, Q. Yu, X. Zhong, M. Li, C. Ikonomidou, and L. Li* (2018). Isobaric Multiplex reagents for carbonyl containing compound (SUGAR) high-throughput quantitative analysis. Under review. G. Li, K. DeLaney, and L. Li* (2018). An integrative chiral anatomy platform (iCAP) for chiralitydependent Abeta oligomerization and recognition. To be submitted. G. Li, F. Ma, Q. Cao, and L. Li* (2018). Highly efficient and high throughput peptide/protein labeling and desalting via nanosecond photochemical reaction. Manuscript in preparation. G. Li, Q. Cao, Y. Liu, K. DeLaney, Z. Tian, E. Moskovets, L. Li* (2018). Characterizing and alleviating Ion suppression effects in atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDI).Rapid Communication in Mass Spectrometry. Under revision. I. Sibilska, Y. Feng, L. Li and J. Yin* (2018). Amino acid activation by trimetaphosphate expands de Novo peptide synthesis from abiotic to prebiotic environments. Origins of Life and Evolution of Biospheres. Published on-line. https://doi.org/10.1007/s11084-018-9564-7 J. Zhu, Z. Chen, J. Zhang, M. An, J. Wu, Q. Yu, S.J. Skilton, M. Bern, I. Sen, L. Li, and D. M. Lubman* (2018). Quantitative LC-EThcD-MS/MS determination of site-specific N-glycopeptides in serum haptoglobin between hepatocellular carcinoma and cirrhosis. Journal of Proteome Research. Under revision. Z. Chen, Q. Yu, J. Johnson, R. Shipman, X. Zhong, J. Huang, H. Zetterberg, S. Asthana, C. Carlsson, O. Okonkwo, and L. Li* (2018). In-depth site-specific O-glycosylation analysis of glycoproteins and endogenous peptides in cerebrospinal fluid (CSF) across Alzheimer’s disease spectrum. Journal of Proteome Research. To be submitted. J. Huang, J. Dong, X. Shi, Z. Chen, Y. Cui, M. S. Glover, X. Liu, M. Ye, and L. Li* (2018). Enrichment and separation of phosphopeptides and M6P glycopeptides by Ti(IV)-IMAC in a typical HILIC-mode elution. ACS Chemical Biology. To be submitted. Y. Gao, B.E. Eisinger, C. Yang, L.E. Kelnhofer, C. Rojo, Y. Sun, C. Giuliani, D. P. Magyar, E. M. Jobe, S. Qian, R. Li, S. Keles, L. Li, Q. Chang, X. Zhong, and X. Zhao* (2018). Integrative genome-wide analyses reveal a role for MBD1 in maintaining chromatin accessibility of neurodevelopmental genes. Nature Neuroscience, Under review. X. Zhong, J. Wang, C. Carlsson, O. Okonkwo, H. Zetterberg, and L. Li* (2018). Discovery and verification of candidate biomarkers in cerebrospinal fluid of preclinical Alzheimer’s disease. Frontiers in Molecular Neuroscience. Invited Contribution, Under review. Z. Chen, M.S. Glover, and L. Li* (2018). Recent advances in ion mobility-mass spectrometry for Improved structural characterization of glycans and glycoconjugates. Current Opinion in Chemical Biology. 42, 1-8. Epub Oct 25, 2017, doi: 10.1016/j.cbpa.2017.10.007. Invited Review for Special Focus Issue in the Omics series covering the topic of Ion Mobility-Mass Spectrometry.