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个人简介

高蓓,女,1982年10月生,博士,华东理工大学生物工程学院硕士生导师。长期致力于合成生物学、分子生物学、生物催化与生物转化等领域相关研究工作。作为负责人先后主持国家科技部“863”计划、国家自然科学基金、上海市自然科学基金、中央高校基本科研业务费探索基金等多项国家及省部级课题,并与恒瑞医药股份有限公司、汉康豆类食品有限公司等多家企业进行合作。在“Scientific Reports”、“Catalysis Science and Technology”、“FEBS Journal”、“Journal of Biotechnology”等本领域重点期刊上发表论文多篇,授权专利多项。 教育经历 2005-2010 华东理工大学博士 2001-2005 燕山大学学士

研究领域

合成生物学:基于合成生物学和代谢工程的原理和方法,致力于微生物细胞工厂的开发与应用; 酶工程:基于计算生物学和结构生物学的酶资源开发与理性改造;基于生物化学和生物催化与转化的工业化酶的创制及应用技术研究。

近期论文

查看导师新发文章 (温馨提示:请注意重名现象,建议点开原文通过作者单位确认)

1.Min Jiang, Lujia Zhang, Fengqing Wang, Jie Zhang, Guosong Liu, Bei Gao, Dongzhi Wei. Novel application of magnetic protein: convenient one-step purification and immobilization of proteins. Scientific Reports. 2017, DOI:10.1038/s41598-017-13648-x. 2.BeiGao, Shuiqin Jiang, Liuzhu Wang, Lujia Zhang, Dongzhi Wei. Energy and conformation determine the enantioselectivity of enzyme. Biochemical Engineering Journal. 2018, 106-112. 3.Lei He, Youzhi Mao, Lujia Zhang, Hualei Wang, SitiAisyah Alias, Bei Gao, Dongzhi Wei. Functional expression of a novel α-amylase from Antarctic psychrotolerant fungus forbaking industry and its magneticimmobilization. BMC Biotechnology. 2017, 17:22. 4.Shuiqin Jiang, Lujia Zhang, Zhiqiang Yao, Bei Gao, Hualei Wang, Dongzhi Wei. Switching a nitrilase from Syechocystis sp. PCC6803 to a nitrile hydratase by rationally regulating reaction pathways. Catalysis Science and Technology. 2017, 7(5):1122-1128. 5.Shuiqin Jiang, Lujia Zhang, Dongbin Cui, Zhiqiang Yao, Bei Gao, Jinping Lin, Dongzhi Wei. The important role of halogenbond in substrate selectivity ofenzymatic catalysis. Scientific Reports. 2016, 6: 34750. 6.Zhiqiang Yao, Lujia Zhang, Bei Gao, Dongbing Cui, Fengqing Wang, Xiao He, Dongzhi Wei. A semiautomated structure-based method to predict substrates of enzymes via molecular docking: a case study with Candida antarctica lipase B. Journal of Chemical Information and Modeling. 2016, 56: 1979-1994. 7.Wenyuan Gao, Kai Wu, Lifeng Chen, Haiyang Fan, Zhiqiang Zhao, Bei Gao, Hualei Wang, Dongzhi Wei. A novel esterase from a marine mud metagenomics library for biocatalytic synthesis of short chain flavor esters. Microbial Cell Factories. 2016, 15: 41-52 8.BeiGao, Youzhi Mao, Lujia Zhang, Lei He, Dongzhi Wei. A novel saccharifying α-amylase of Antarctic psychrotolerant fungi Geomycespannorum: Gene cloning, functional expression, and characterization.Starch/Stärke. 2015, 67(1): 1-9. 9.Youzhi Mao, Yanchen Yin, Lujia Zhang, SitiAisyah Alias, Bei Gao , Dongzhi Wei. Development of a novel Aspergillus uracil deficient expression systemand its application in expressing a cold-adapted α-amylase gene fromAntarctic fungi Geomycespannorum. Process Biochemistry. 2015, 50: 1581-1590 10.Yanchen Yin, Youzhi Mao, Xiaolie Yin, BeiGao, Dongzhi Wei. Construction of a shuttle vector for heterologous expression of a novel fungal α-amylase gene in Aspergillus oryzae.Journal of Microbiology and Biotechnology. 2015,25(7):988-998. 11.Dongbing Cui,Lujia Zhang, Shuiqin Jiang,Zhiqiang Yao,Bei Gao,Jinping Lin,Y. Adam Yuan,Dongzhi Wei. A computational strategy for altering an enzyme in its cofactor preference to NAD(H) and/or NADP(H). FEBS Journal. 2015, 282(12): 2339-2351. 12.Zhenggui He, Lujia Zhang, Youzhi Mao, JingchaoGu, Qi Pan, Sixing Zhou, Bei Gao,Dongzhi Wei. Cloning of a novel thermostable glucoamylasefrom thermophilic fungus Rhizomucorpusillus andhigh-level co-expression withα-amylase in Pichiapastoris. BMC Biotechnology. 2014, 14:114 13.Zhenggui He, Yanchen Yin, Youzhi Mao, Sixing Zhou, Qi Pan, Dongzhi Wei, Bei Gao. Cloning and high-level expression of thermostable α-amylase gene from Rhizomucorpusillus. Journal of Southern Agriculture. 2014, 45(2): 165-172 14.Lujia Zhang,Xiaomang Tang,Dongbing Cui, Zhiqiang Yao,Bei Gao,Shuiqin Jiang,Bo Yin,Y. Adam Yuan,Dongzhi Wei. A method to rationally increase protein stability based on the charge-charge interaction, with application to lipase LipK107. Protein Science. 2014, 23:110-116. 15.Lujia Zhang, Bei Gao, Zuanning Yuan, Xiao He, Y. Adam Yuan, John Z.H. Zhang,Dongzhi Wei. Structure,mechanism, and enantioselectivity shifting of lipase LipK107 with a simple way.BiochimicaetBiophysicaActa (BBA) -Proteins and Proteomics. 2014, 1844:1183-1192. 16.Xiangping Wu, Pengyong You, Erzheng Su, Jingjing Xu, BeiGao,Dongzhi Wei. In vivo functional expression of a screenedP. aeruginosachaperone-dependent lipase in E. coli.BMC Biotechnology, 2012, 12: 58-64. 17.Bei Gao, Tao Xu, Jinping Lin, Lujia Zhang, Zhengbing Jiang, Dongzhi Wei. Improving the catalytic activity of lipase LipK107 from Proteus sp. by site-directed mutagenesis in the lid domain based on computer simulation. Journal of Molecular Catalysis B: Enzymatic, 2011, 68: 286–291. 18.Huiting Song, Lichao Zhou, Lujia Zhang, Bei Gao,Dongzhi Wei, Yaling Shen, Rui Wang, Catherine Madzak, Zhengbing Jiang, Construction of a whole-cell catalyst displaying a fungal lipase for effectivetreatment of oily wastewaters. Journal of Molecular Catalysis B: Enzymatic.2011, 71:166-170. 19.Tao Xu, Bei Gao, Lujia Zhang, Jingpin Lin, Xuedong Wang, Dongzhi Wei. Template-based modeling of a psychrophilic lipase: conformational changes, novel structural features and its application in predicting the enantioselectivity of lipase catalyzed transesterification of secondary alcohols. BiochimicaetBiophysicaActa(BBA) - Proteins & Proteomics, 2010, 1804: 2183-2190. 20.BeiGao, Erzheng Su, Jinping Lin, Dongzhi Wei. Development of recombinant Escherichia coli whole-cell biocatalyst expressing a novel alkaline lipase-coding gene from Proteus sp. for biodiesel production.Journal of Biotechnology, 2009, 139(2):169-175. 21.Zhengbing Jiang, Bei Gao,Ren Ren, Dongzhi Wei.Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparision with the LipB52 displayed on Saccharomyces cereisiae cell surface.BMC Biotechnology, 2008, 8:4: 1-7.

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