蔡孟浩 - 华东理工大学 - 生物工程学院

个人简介

男，博士，副教授，华东理工大学生物工程学院。2006年毕业于华东理工大学理工优秀生部，获工学学士学位；2009年-2010年英国布里斯托大学化学院联合培养博士生；2011年获得华东理工大学生物化工博士学位。2011年-2014年，华东理工大学师资博士后、助理研究员，2014年任职副教授、硕导，2019年破格提为博导。获得上海市青年科技启明星计划，华东理工大学青年英才培育计划,生物工程学院青年英才培养计划，上海市研究生创新计划资助。担任中国微生物学会海洋微生物学专业委员会委员（2016-2021年），第四届中国生物化学与分子生物学学会海洋生物化学与分子生物学分会理事（2016-2020年），上海市生物工程学会合成生物学专业委员会委员（2018-2022年），中国药学会海洋药物专业委员会“中国海洋药物博士论坛”青年委员会委员（2015-2019年）。作为项目负责人，主持国家重点研发计划课题、国家自然科学基金、国家重大新药创制合作课题、国家863课题、上海科技创新行动计划项目、中央高校基本科研业务费专项基金、企业横向合作项目等多项课题。在Metabolic Eng、J Biol Chem、Biotechnol Biofuels等权威期刊发表SCI论文40余篇，第一或通讯作者30余篇。申请中国发明专利18项，已授权8项。担任Microb Cell Fact、Appl Microbiol Biotechnol、FEMS Yeast Res、BMCBiotechnol、Biochem Eng J等杂志审稿人。主讲本科生抗体工程课程、研究生基因工程制药课程，参讲本科生分子生物学（英文）、博士生微生物细胞信号转导（英文）课程。教育经历： 2009.10-2010.10 英国布里斯托大学有机与生物化学联合培养博士 2006.9-2011.3 华东理工大学生物化工博士 2002.9-2006.6 华东理工大学理工优秀生部学士工作经历： 2014.9-至今，华东理工大学副教授，生物工程学院 2013.8-2014.8，华东理工大学助理研究员，生物工程学院 2011.7-2013.7，华东理工大学师资博士后，生物工程学院/药学院

研究领域

主要研究方向为微生物药物合成生物学，旨在利用异源底盘细胞重构天然药物合成途径或改造/调控原生宿主的药物合成途径，实现天然药物的微生物高效发酵生产，解决其来源受限、难制备、成本高等问题。研究内容(一) 新型底盘细胞设计与检验：聚焦天然药物合成酶适配能力强的真核底盘宿主(酵母/曲霉)，解析重要启动子的转录调控机制，以此挖掘特色生物元件并重构转录调控器件(库)，以此设计开发高效、多样化的正交/交互生物合成底盘系统，以满足天然药物生物合成中的不同需求。研究内容(二) 药物分子生物合成与调控：运用分子生物学及组合生物化学手段，异源(酵母/曲霉)重构或同源(曲霉)改造/调控天然药物合成途径，设计组合静态控制及基于特色元/器件设计的动态控制策略，优化调控模式及平衡代谢网络，以此实现天然药物分子在微生物细胞工厂中的高效发酵生产。

近期论文

查看导师新发文章（温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

"Liu Y, Bai C,Liu Q, Xu Q, Qian Z, Peng Q, Yu J, Xu M, Zhou X, Zhang Y, Cai M*.Engineered ethanol-driven biosynthetic system for improveing production of acetyl-CoA derived drugs in Crabtree-negative yeast. Metab Eng. 2019, 54: 275-284. XuQ, Bai C, Liu Y, Song L, Tian L, Yan Y, Zhou J, Zhou X, Zhang Y, Cai M*. Modulation of acetate utilization in Komagataella phaffii by metabolic engineering of tolerance and metabolism. Biotechnol Biofuels. 2019,12:61. Liu Y, Tu X, Xu Q, Bai C, Kong C, Liu Q, Yu J, Peng Q, Zhou X, Zhang Y, Cai M*. Engineered monoculture and co-culture of methylotrophic yeast for de novo production of monacolin J and lovastatin from methanol. Metab Eng. 2018, 45:189-199. Kong C, Huang H, Xue Y, Liu Y, Peng Q, Liu Q, Yin Y, Zhou X, Zhang Y, Cai M*. Heterologous pathway assembly reveals molecular steps of fungal terreic acid biosynthesis. 2018,Sci Rep. 2018:2116. Wang J, Wang X, Shi L, Zhang Y, Zhou X*, Cai M*. Reduced methanol input induces increased protein output by AOX1 promoter in a trans‑acting elements engineered Pichia pastoris. J Ind Microbiol Biotechnol. 2018, 45:25-30. Shi L, Wang X, Wang J, Zhang P, Qi F, Cai M*, Zhang Y, Zhou X*. Transcriptome analysis of Δmig1Δmig2 mutant reveals their roles in methanol catabolism, peroxisome biogenesis and autophagy in methylotrophic yeast Pichia pastoris. 2018, Gen. Genom. 40:399–412. Liu Y, Bai C, Xu Q, Yu J, Zhou X, Zhang Y, Cai M*. Improved methanol‑derived lovastatin production through enhancement of the biosynthetic pathway and intracellular lovastatin efflux in methylotrophic yeast. Bioresour. Bioprocess. 2018,5:22 Wang J, Wang X, Shi L, Qi F, Zhang P, Zhang Y, Zhou X*, Song Z*, Cai M*. Methanol-independent protein expression by AOX1 promoter with trans-acting elements engineering and glucose-glycerol-shift induction in Pichia pastoris.Sci Rep. 2017, 7:41850. Xue Y, Kong C, Shen W, Bai C, Ren Y, Zhou X, Zhang Y, Cai M*. Methylotrophic yeast Pichia pastoris as a chassis organism for polyketide synthesis via the full citrinin biosynthetic pathway. J Biotechnol. 2017, 242:64-72. Wu Y, Ren Y, Zhou X, Cai M*, Zhang Y*. Transcription factor Agseb1 affects development, osmotic stress response, and secondary metabolism in marine-derived Aspergillus glaucus. J. Basic Microbiol. 2017, 57:873-882 (cover article). Huang M, Gao Y, Zhou X, Zhang Y, Cai M*. Regulating unfolded protein response activator HAC1p for production of thermostable raw-starch hydrolyzing α-amylase in Pichia pastoris.Bioproc. Biosyst. Eng. 2017, 40:341-350. Gao Y, Huang M, Sun X, Zhang X, Zhang Y, Zhou X*, Cai M*. Single-site mutation of C363G or N463T strengthens thermostability improvement of IG181-182 deleted acidic α-amylase from deep-sea thermophile Geobacillus sp. Food Biotechnol. 2017, 31:57-71. Xiao S, Gao Y, Wang X, Shen W, Wang J, Zhou X, Cai M*, Zhang. Peroxisome-targeted and tandem repeat multimer expressions of human antimicrobial peptide LL37 in Pichia pastoris. Prep. Biochem. Biotechnol. 2017, 47:229-235. Liu S, Li J, Wu Y, Ren Y, Liu Q, Wang Q, Zhou X, Cai M*, Zhang Y. De novo transcriptome sequencing of marine-derived Aspergillus glaucus and comparative analysis of metabolic and developmental variations in response to salt stress. Gen. Genom. 2017, 39:317-329. Wang X, Wang Q, Wang J, Bai P, Shi L, Shen W, Zhou M, Zhou X, Zhang Y, Cai M*. Mit1 transcription factor mediates methanol signaling and regulates alcohol oxidase 1 promoter in Pichia pastoris. J Biol Chem. 2016, 291:6245-6261. Zhang X, He H, Yin Y, Zhou W, Cai M*, Zhou X, Zhang Y. A light–dark shift strategy derived from light-responded metabolic behaviors for polyketides production in marine fungus Halorosellinia sp.. J Biotechnol. 2016, 221:34-42. He H, Xue Y, Xu N, Yin Y, Lu J, Cai M*, Zhang Y. A novel pH–precursor–glucose synergic control strategy for dinactin biosynthesis by an arctic actinomycete Streptomyces sp. Process Biochem. 2016, 51:1911-1918. Shen C, Xu N, Gao Y, Sun X, Yin Y*, Cai M*, Zhou X, Zhang Y. Stimulatory effect of ethanol on libertellenone H biosynthesis by Arctic fungus Eutypella sp. D-1. Bioproc. Biosyst. Eng. 2016, 39:353-360. Wang X,Cai M*, Shi L, Wang Q, Zhu J, Wang J, Zhou M, Zhou X, Zhang Y. PpNrg1 is a transcriptional repressor for glucose and glycerol repression of AOX1 promoter in methylotrophic yeast Pichia pastoris. Biotechnol. Lett. 2016, 38:291-298. Jiang T, Cai M*, Huang M, He H, Lu J, Zhou X, Zhang Y. Bioprocess exploration for thermostable α-amylase production of a deep-sea thermophile Geobacillus sp. in high temperature bioreactor. Prep. Biochem. Biotechnol. 2016, 46:620-627. Wang M, He H, Na K, Cai M*, Zhou X, Zhao W, Zhang Y. Designing novel glucose/ornithine replenishment strategies by biosynthetic and bioprocess analysis to improve fibrinolytic FGFC1 production by the marine fungus Stachybotrys longispora. Process Biochem. 2015, 50:2012-2018"