吴辉 - 华东理工大学 - 生物工程学院

个人简介

吴辉，工学博士，副教授。现任中国轻工业生物基材料工程重点实验室副主任，中国化工学会生物化工专委会青年学者工作委员会委员，中国生物工程学会青年工作委员会委员，上海市生物工程学会合成生物学委员会委员，生物反应器工程国家重点实验室固定人员。主要研究方向是微生物代谢工程、代谢调控与和合成生物学。教育部“霍英东青年教师基金”获得者（2018）。2004年毕业于南京工业大学生物工程系，获工学学士学位。2009年毕业于华东理工大学发酵工程专业，获工学博士学位。同年进入中科院上海生命科学研究院植物生理生态研究所从事博士后研究工作。2011年来到誉有“南方的哈佛”之称的美国莱斯大学生物工程系从事博士后研究工作。2014年9月引进华东理工大学生物工程学院。入选华东理工大学“青年英才培育计划”（2018 A类、2015）等。2019年被破格遴选为博士生导师。作为负责人主持国家自然科学基金面上项目、青年项目、教育部“霍英东青年教师基金” 、上海市自然科学基金等项目；作为主要研究骨干参与科技部国家重点研发计划课题、863计划等。近年来以第一作者或通讯作者在Metabolic Engineering，Biotechnology Advances，ACS Synthetic Biology，Biotechnology and Bioengineering，Applied and Environmental Microbiology等本领域重点期刊上发表SCI收录论文30余篇。申请美国专利6 项，已授权3项。申请中国发明专利9 项，已授权3项。指导研究生获2018年度“华东理工大学-东阿阿胶杰出奖学金”，华东理工大学第十三届“华陆科技·奋进杯”大学生课外学术科技作品竞赛二等奖；本科生获2017年和2018年连续两次获得iGEM竞赛金奖，“大学生创新创业训练计划”上海市级项目。

研究领域

1．微生物代谢工程生产平台化合物和生物活性物质运用生物信息学及代谢工程的最新技术和方法，合理设计及重构微生物代谢途径，结合代谢调控策略，建立以可再生生物质资源（糖、甘油、乙酸、甲酸、CO2等）为原料的新型高效微生物细胞工厂，合成多种生物基化学品与生物基材料，如丁二酸，3-羟基丙酸，丙酮，生物聚酯，氨基酸，脂肪酸及其衍生物等。 2．合成生物学中“代谢晶体管”研究及其应用 “代谢晶体管”控制模式借鉴电子元器件“晶体管”的放大控制模式，通过调节细胞中微量关键物质的浓度，进而对整个细胞进行代谢重编程。 3. 基于微生物电化学合成的代谢工程与合成生物学运用代谢工程与合成生物学的策略，结合电化学平台技术与方法，搭建能利用一碳资源的自养型大肠杆菌细胞工厂平台，用于生产多种生物基化合物如丙酮，异丙醇，3-羟基丙酸，丁二酸等。

近期论文

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1．Hao Yang#, Bing Huang#, Zhimin Li, Qin Ye, Hui Wu*. 2019. Metabolic engineering of Escherichia coli carrying the hybrid acetone-biosynthesis pathway for efficient acetone biosynthesis from acetate. Microbial Cell Factories.18:9.(IF=4.402, JCR Q1) 2. Jingxian Lu, Zhimin Li, Qin Ye, Hui Wu*. 2019. Effect of reducing the activity of respiratory chain on biosynthesis of poly(3-hydroxybutyrate-co-lactate) in Escherichia coli. Chinese Journal of Biotechnology 35(1):59-69 3．Boliang Gao, Li Li, Hui Wu, Du Zhu, Min Jin, Wu Qu, Runying Zeng*. 2019. A novel strategy for efficient agaro-oligosaccharide production based on the enzymatic degradation of crude agarose in Flammeovirga pacifica WPAGA1. Frontiers in Microbiology. DOI: 10.3389/fmicb.2019.01231. (IF=4.259, JCR Q1) 4．Xiangwei Cui, Junxian Wan, Xing Zhang, Hui Wu, Zhimin Li*, Qin Ye. 2019. Efficient glutathione production in metabolically engineered Escherichia coli strains using constitutive promoters. Journal of Biotechnology. 289: 39-45 (IF=3.163, JCR Q2) 5．Jiapeng Tang, Zhenqing Qian, Hui Wu*. 2018. Enhancing cordycepin production in liquid static cultivation of Cordyceps militaris by adding vegetable oils as the secondary carbon source. Bioresource Technology. 268: 60-67. (IF=6.669, JCR Q1) 6．Bing Huang, Hao Yang, Guochen Fang, Xing Zhang, Hui Wu*, Zhimin Li*, Qin Ye. 2018. Central pathway engineering for enhanced succinate biosynthesis from acetate in Escherichia coli. Biotechnology and Bioengineering. 115:943-954. (*Co-corresponding) (IF=4.26, JCR Q1) 7．Wei Li#, Hui Wu#, Mai Li, Ka-Yiu San*. 2018. Effect of NADPH availability on free fatty acid production in E. coli. Biotechnology and Bioengineering. 115:444-452. (#First two authors contributed to this paper equally) (IF=4.26, JCR Q1) 8．Han Liu, Guochen Fang,Hui Wu*, Zhimin Li*, Qin Ye. 2018. L-cysteine production in Escherichia coli based on rational metabolic engineering and modular strategy. Biotechnology Journal. 13:e1700695. (*Co-corresponding) (IF=3.543, JCR Q2) 9. Feng Li#, Yuanxiu Li#, Liming Sun, Xiaoli Chen, Xingjuan An, Changji Yin, Yingxiu Cao, Hui Wu, Hao Song*. 2018. Modular engineering intracellular NADH regeneration boosts extracellular electron transfer of Shewanella oneidensis MR-1. ACS Synthetic Biology. 7(3):885-895. (IF=5.571, JCR Q1) 10．Qing Li#, Bing Huang#, Qiaofei He, Jingxian Lu, Xun Li, Zhimin Li, Hui Wu*, Qin Ye. 2018. Production of succinate from simply purified crude glycerol by engineered Escherichia coli using two-stage fermentation. Bioresources and Bioprocessing. 5:41. (#First two authors contributed to this paper equally) (Feature Article) 11. Jiaqi Jiang, Bing Huang, Hui Wu*, Zhimin Li*, Qin Ye. Efficient 3-hydroxypropionic acid production from glycerol by metabolically engineered Klebsiella pneumoniae. Bioresources and Bioprocessing. 5:34. (*Co-corresponding) 12．Qing Li, Bing Huang, Hui Wu*, Zhimin Li, Qin Ye. 2017. Efficient anaerobic production of succinate from glycerol in engineered Escherichia coli by using dual carbon sources and limiting oxygen supply in preceding aerobic culture. Bioresource Technology. 231: 75-84. (IF=6.669, JCR Q1) 13．Xing Zhang#, Hui Wu#, Bing Huang, Zhimin Li*, Qin Ye. 2017. One-pot synthesis of glutathione by a two-enzyme cascade using a thermophilic ATP regeneration system. Journal of Biotechnology. 241:163-169. (# First two authors contributed to this paper equally) (IF=3.163, JCR Q2) 14．Qing Li#, Hui Wu#, Zhimin Li, Qin Ye*. 2016. Enhanced succinate production from glycerol by engineered Escherichia coli strains. Bioresource Technology. 218: 217-223. (# First two authors contributed to this paper equally) (IF=6.669, JCR Q1) 15．Yunjie Li, Bing Huang, Hui Wu*, Zhimin Li*, Qin Ye, Yi-Heng Percival Zhang. 2016. Production of succinate from acetate by metabolically engineered Escherichia coli. ACS Synthetic Biology. 5:1299-1307. (*Co-corresponding) (IF=5.571, JCR Q1) 16．Jianhua Yang, Wei Li, Dezheng Wang, Hui Wu*, Zhimin Li*, Qin Ye. 2016. Characterization of bifunctional L-glutathione synthetases from Actinobacillus pleuropneumoniae and Actinobacillus succinogenes for efficient glutathione biosynthesis. Applied Microbiology and Biotechnology. 100(14): 6279-6289. (*Co-corresponding) (IF=3.670, JCR Q2) 17．Jing Zhang, Cong Quan, Cheng Wang, Hui Wu*, Zhimin Li*, Qin Ye. 2016. Systematic manipulation of glutathione metabolism in Escherichia coli for improved glutathione production. Microbial Cell Factories. 15:38. (*Co-corresponding) (IF=4.402, JCR Q1) 18．Zhuan Cheng, Jiaqi Jiang, Hui Wu, Zhimin Li*, Qin Ye. 2016. Enhanced production of 3-hydroxypropionic acid from glucose via malonyl-CoA pathway by engineered Escherichia coli. Bioresource Technology. 200: 897-904.(IF=6.669, JCR Q1) 19．Dezheng Wang, Cheng Wang, Hui Wu*, Zhimin Li*, Qin Ye. 2016. Glutathione production by recombinant Escherichia coli expressing bifunctional glutathione synthetase. Journal of Industrial Microbiology and Biotechnology. 43:45-53. (*Co-corresponding) (IF=2.993, JCR Q2) 20．Qin Ye*, Zhimin Li, Hui Wu. 2016. Principle and performance of gas self-inducing reactors and applications to biotechnology. Advances in Biochemical Engineering/Biotechnology. 152:1-33.(IF=2.341, JCR Q2)