李文利 - 中国海洋大学

个人简介

李文利，博士，教授，博士生导师，院长助理。教育部“新世纪优秀人才”和中国海洋大学“英才工程”第一层次资助计划获得者。现任中国微生物学会永久会员、中国海洋湖沼学会药学分会理事、《中国海洋药物》杂志编委。2003年毕业于中科院微生物研究所，获博士学位，主要从事核苷肽类抗生素生物合成研究；之后在美国威斯康辛大学麦迪逊分校药学院从事博士后研究，主要从事聚酮类化合物及烯二炔类抗生素生物合成研究；2008年进入美国PPD生物制药公司从事新药研发；2009年10月加入中国海洋大学医药学院，建立了海洋天然产物生物合成实验室。2010年1月入选教育部“新世纪优秀人才”和中国海洋大学“英才工程”第一层次资助计划。

研究领域

以海洋微生物来源的药物先导物为目标分子，从基因和蛋白水平揭示其生物合成途径及其调控机制；在此基础上，采用代谢工程方法与策略提高目标化合物的产量，并理性设计和创造出“非天然”的化合物。主要包括： 1、海洋药物先导物生物合成研究； 2、海洋药物先导物组合生物合成（Combinatorial biosynthesis）与代谢工程； 3、基于基因组采掘（Genome mining）的新型海洋药物先导物的发现。

近期论文

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1.Zhang Y.; Huang H.; Xu S.; Wang B.; Ju J.; Tan H.; Li W.* . Activation and enhancement of Fredericamycin A production in deepsea-derived Streptomyces somaliensis SCSIO ZH66 by using ribosome engineering and response surface methodology. Microb Cell Fact. 2015, DOI: 10.1186/s12934-015-0244-2. 2.Che Q.#; Li T.#; Liu X.; Yao T.; Li J.; Gu Q.; Li D.*, Li W.*; Zhu T*. Genome scanning inspired isolation of reedsmycins A-F, polyene-polyol macrolides from Streptomyces sp. CHQ-64. RSC Adv. 2015, 5, 22777-22782. 3.Ding X.; Chen C.; Cui Q.; Li W.*; Feng Y. Resonance assignments of the periplasmic domain of a cellulose-sensing trans-membrane anti-sigma factor from Clostridium thermocellum. Bimol NMR Assign. 2015, DOI: 10.1007/s12104-015-9601-7. 4.Song X.; Wang X.; Tan Y.; Feng Y.; Li W.; Cui Q.High production of squalene using a newly isolated yeast-like strain Pseudozymasp. SD301. J. Agric. Food Chem.2015,DOI: 10.1021/acs.jafc.5b03539. 5.Qin W.; Liu Y.; Ren P.; Zhang J.; Li H.; Tian L.;Li W*. Uncovering a glycosyltransferase provides insights into the glycosylation step during macrolactins and bacillaenes biosynthesis. ChemBioChem, 2014, 15(18), 2747-2753. 6.Liu Y.; Zheng H.; Qin W.; Zhan G.; Tian L; Li W*. Establishment of an efficient transformation protocol and its application in marine-derived Bacillus strain. Sci. China Life Sci. 2014, 57, 627-635. 7.Li T.; Du Y.; Cui Q.; Zhang J.; Zhu W.; Hong K.*; Li W*.Cloning, characterization and heterologous expression of the indolocarbazole biosynthetic gene cluster from marine-derived Streptomyces sanyensisFMA. Mar. Drugs 2013, 11, 466-488. 8.Gao M.; Song X.; Feng Y.; Li W.; Cui Q. Isolation and characterization of Aurantiochytrium species: high docosahexaenoic acid (DHA) production by the newly isolated microalga,Aurantiochytriumsp. SD116. J Oleo Sci. 2013, 62(3), 143-151. 9.Cui G.; Hong W.; Zhang J.; Li W.; Feng Y.; Liu C.; Cui Q. Targeted gene engineering in Clostridium cellulolyticum H10 without methylation. J. Microbiol. Methods. 2012, 89, 201-208. 10.Yang D.; Li W.; Huang S.; Shen B. Functional characterization of ttnI completing the tailoring steps for tautomycetin biosynthesis in Streptomyces griseochromogenes. Org. Lett. 2012, 14(5), 1302-1305. 11.Luo Y.; Li W.; Ju J.; Yuan Q.; Peter N.R.; Hoffmann F.M.; Huang S.; Bugni T.S.; Rajski S.; Osada H.; Shen B. Functional characterization of TtnD and TtnF, unveiling new insights into tautomycetin biosynthesis. J. Am. Chem. Soc. 2010, 132, 6663-6671. 12.Ju J.#; Li W.#; Yuan Q.; Peters N.R.; Hoffmann F.M.; Rajski S.R.; Shen B. Functional characterization of ttmMunveils new tautomycin analogs and insight into tautomycin biosynthesis and activity. Org. Lett. 2009, 11(7), 1639-1642. (#Contributed equally). 13.Li W.; Luo Y.; Ju J.; Rajski S.R.; Osada H.; Shen B. Characterization of the tautomycetin biosynthetic gene cluster from Streptomyces griseochromogenes." J. Nat. Prod. 2009, 72, 450-459. 14.Lin S.; Geoffrey P.H.; Chen Y.; Li W; Shen B. Characterization of the SgcF epoxide hydrolase supporting an (R)-vicinal diol intermediate for enediyne antitumor antibiotic C-1027 biosynthesis. J. Am. Chem. Soc. 2009, 131, 16410-16417. 15.Pinchot, S.N.; Adler, J.T.; Luo, Y.; Ju, J.; Li W; Shen, B; Kunnimalaiyaan, M.; Chen, H. Tautomycin suppresses growth and neuroendocrine hormone markers in carcinoid cells through activation of the Raf-1 pathway. Am. J. Surg. 2009, 197(3), 313-319. 16.Li W.; Ju J.; Osada H.; Shen B. Characterization of the tautomycin biosynthetic gene cluster from Streptomyces spiroverticillatusunveiling new insights into dialkylmaleic anhydride and polyketide biosynthesis." J. Bio. Chem. 2008, 283(42), 28607-28617.