Abraham, John M. - Johns Hopkins University

个人简介

For the last several years, Dr. Abraham has been investigating and developing potential new small molecule cancer therapeutics. Libraries of small peptides were prepared using recombinant means for screening assays which included binding to and the transfer of radioactivity to various human cancer cell lines in culture. Certain decapeptides with demonstrated binding and transfer potential were chemically synthesized and further studied. The majority of his research work over the last five years has centered on investigating and assaying the inhibition of growth of human-derived xenografted tumors in immunocompromised mice (nude mice and NOD/SCID). The discovery of a method to strongly inhibit xenografted tumor growth has recently been published, and the JHU Technology Transfer Office submitted a provisional patent application based on this work to the US Patent and Trademark Office in June 2013.

研究领域

Previously, the majority of my experimental work utilized siRNA knockdown techniques and microarray technology to identify and characterize downstream genetic targets of regulator genes. Recently, I have been involved in identifying novel decapeptides that, when labeled with 32P radioisotope, can transfer this tumor-ablating agent to colon and esophageal adenocarcinoma cells.

近期论文

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1. Blount PL, Galipeau PC, Sanchez CA, Neshat K, Levine DS, Yin J, Suzuki H, Abraham JM, Meltzer SJ, Reid BJ. 17p allelic losses in diploid cells of patients with Barrett's esophagus who develop aneuploidy. Cancer Res. 1994; 54:2292-2295 2. Meltzer SJ, Yin J, Manin B, Rhyu M-G, Cottrell J, Hudson E, Redd JL, Krasna MJ, Abraham JM, Reid BJ. Microsatellite instability occurs frequently and in both diploid and aneuploid cell populations of Barrett's-associated esophageal adenocarcinomas. Cancer Res. 1994; 54:3379-3382. 3. Suzuki H, Harpaz N, Tarmin Y, Yin J, Jiang H-Y, Bell JD, Hontanosas M, Groisman GM, Abraham JM, Meltzer SJ. Microsatellite instability in ulcerative colitis-associated colorectal dysplasias and cancers. Cancer Res. 1994; 54:4841-4844. 4. Harpaz N, Peck AL, Yin J, Fiel I, Hontanosas M, Tong TR, Laurin JN, Abraham JM, Greenwald BD, Meltzer SJ. p53 protein expression in ulcerative colitis-associated colorectal dysplasia and carcinoma. Human Pathol. 1994; 25:1069-1074. 5. Zhou X, Tarmin L, Yin J, Jiang H-Y, Suzuki H, Rhyu M-G, Abraham JM, Meltzer SJ. The MTS1 gene is frequently mutated in primary human esophageal tumors. Oncogene 1994; 9:3737-3741. 6. Tarmin L, Yin J, Zhou X, Suzuki H, Jiang H-Y, Abraham JM, Krasna MJ, Cottrell J, Meltzer SJ. Frequent loss of heterozygosity on chromosome 9 in adenocarcinoma and squamous cell carcinoma of the esophagus. Cancer Res. 1994; 54:6094-6096. 7. Balcer-Kubiczek EK, Yin J, Lin K, Harrison GH, Abraham JM, Meltzer SJ. p53 mutational status and survival of human breast cancer MCF7 cell variants after exposure to X-rays or fission neutrons. Radiation Res. 1995; 142:256-262. 8. Zhou X, Suzuki H, Shimada Y, Imamura M, Yin J, Jiang H-Y, Tarmin L, Abraham JM, Meltzer SJ. Genomic DNA and messenger RNA expression alterations of the CDKN2B and CDKN2 genes in esophageal squamous carcinoma cell lines. Genes, Chromosomes & Cancer 1995; 13:285-290. 9. Suzuki H, Zhou X, Yin J, Lei J, Jiang H-Y, Suzuki Y, Abraham JM, Chan T, Hannon GJ, Mergner WJ, Abraham JM, Meltzer SJ. Intragenic mutations of p15 and p16 in primary human esophageal cancers. Human Molec. Genet. 1995; 4:1883-1888. 10. Berg PE, Liu J, Yin J, Rhyu M-G, Frantz CN, Meltzer SJ. Microsatellite instability is infrequent in neuroblastoma. Cancer Epidemiology, Biomarkers & Prevention 1995; 4:907-909. 11. Abraham JM, Wang S, Suzuki H, Jiang H-Y, Rosenblum-Vos LS, Yin J, Meltzer SJ. Esophagin cDNA cloning and characterization: a tissue-specific member of the small proline-rich protein family that is not expressed in esophageal tumors. Cell Growth & Different. 1996; 7:855-860. 12. Zhou X, Suzuki H, Lei J, Cole K, Yin J, Suzuki Y, Abraham JM, Shimada Y, Imamura T, Chan JT, Hannon GJ, Meltzer SJ. Growth suppression of esophageal cancer cells by p16INK4 and p15INK4B in vitro. Cancer J. Sci. Am. 1996; 2:221-224. 13. Souza RF, Appel R, Yin J, Wang S, Smolinski KN, Abraham JM, Zou T-T, Shi Y-Q, Lei J, Cottrell J, Cymes K, Biden K, Simms L, Leggett B, Lynch PM, Frazier M, Powell SM, Harpaz N, Sugimura H, Young J, Meltzer SJ. Microsatellite instability in the insulin-like growth factor II receptor gene in gastrointestinal tumors. Nature Genet. 1996; 14:255-257. 14. Lei J, Zou T-T, Shi Y-Q, Smolinski KN, Yin J, Souza RF, Appel R, Wang S, Cymes K, Chan O, Harpaz N, Abraham JM, Meltzer SJ. Infrequent DPC4 gene mutation in esophageal cancer, gastric cancer, and ulcerative colitis-associated neoplasms. Oncogene 1996; 13:2459-2462. 15. Balcer-Kubiczek EK, Harrison GH, Zhang X-F, Shi Z-M, Abraham JM, McCready WA, Ampey III LL, Meltzer SJ, Jacobs MC, Davis CC. Rodent cell transformation and immediate early gene expression following 60-Hz magnetic field exposure. Envir. Health Perspect. 1996; 104:1188-1198. 16. Souza RF, Lei J, Yin J, Appel R, Zou T-T, Zhou X, Wang S, Rhyu M-G, Cymes K, Chan O, Park W-S, Krasna MJ, Greenwald BD, Cottrell J, Abraham JM, Harpaz N, Meltzer SJ. A transforming growth factor-?1 receptor type II mutation occurs in ulcerative colitis-associated and sporadic colorectal neoplasms. Gastroenterol. 1997; 112:40-45. 17. Balcer-Kubiczek EK, Meltzer SJ, Han L-H, Zhang X-F, Zhong M-S, Harrison GH, Qin JT, Abraham JM. Csa-19, a radiation-responsive human gene, identified by an unbiased two-gel cDNA library screening method. Oncogene 1997; 14:3051-3058. 18. Souza RF, Yin J, Smolinski KN, Zou T-T, Wang S, Shi Y-Q, Rhyu M-G, Cottrell J, Abraham JM, Biden K, Simms L, Leggett B, Bova GS, Frank T, Powell SM, Sugimura H, Young J, Harpaz N, Shimizu K, Matsubara N, Meltzer SJ. Frequent mutation of the E2F-4 cell cycle gene in primary human gastrointestinal tumors. Cancer Res. 1997; 57:2350-53. 19. Zou T-T, Lei J, Shi Y-Q, Yin J, Wang S, Souza RF, Smolinski KN, Greenwald BD, Abraham JM, Harpaz N, Meltzer SJ. FHIT gene alterations in ulcerative colitis (UC) and esophageal cancer. Oncogene 1997; 15:101-105. 20. Wang S, Souza RF, Kong D, Yin J, Smolinski KN, Zou T-T, Young J, Flanders K, Sugimura H, Abraham JM, Meltzer SJ. Deficient transforming growth factor-?1 activation and excessive insulin-like growth factor II expression in IGFII receptor-mutant tumors. Cancer Res. 1997; 57:2543-2546. 21. Kong D, Suzuki A, Zou T-T, Sakurada A, Kemp LW, Wakatsuki S, Yokoyama T, Yamakawa H, Furukawa T, Sato M, Ohuchi N, Sato S, Yin J, Wang S, Abraham JM, Souza RF, Smolinski KN, Meltzer SJ, Horii A. PTEN1 is frequently mutated in primary endometrial carcinomas. Nature Genet. 1997; 17:143-144. 22. Yin J, Kong D, Wang S, Zou T-T, Souza RF, Smolinski KN, Lynch PM, Frazier M, Hamilton SR, Sugimura H, Powell SM, Young J, Abraham JM, Meltzer SJ. Mutation of the hMSH3 and hMSH6 mismatch repair genes in genetically unstable human colorectal and gastric carcinomas. Human Mutation 1997; 10:474-478. 23. Zou T, Fleisher AS, Kong D, Yin J, Souza RF, Wang S, Smolinski KN, Abraham JM, Meltzer SJ. Sequence alterations of IGFBP-3 in neoplastic and normal gastrointestinal tissues. Cancer Res. 1998; 58:4802-4804. 24. Balcer-Kubiczek EK, Zhang XF, Han LH, Harrison GH, Davis CC, Zhou XJ, Ioffe V, McCready WA, Abraham JM, Meltzer SJ. BIGEL analysis of gene expression in HL60 cells exposed to X rays or 60 Hz magnetic fields. Radiation Res. 1998; 150:663-672. 25. Fleisher AS, Esteller M, Wang S, Tamura G, Suzuki H, Yin Y, Zou T-T, Abraham JM, Kong D, Smolinski KN, Shi Y-Q, Rhyu M-G, Powell SM, James SP, Wilson KT, Herman JG, Meltzer SJ. Hypermethylation of the hMLH1 gene promoter in human gastric cancers with microsatellite instability. Cancer Res. 1999; 59:1090-1095.