何琳 - 华东师范大学 - 生命科学学院

个人简介

工作经历 2023.01-2023.12：澳大利亚麦考瑞大学，应用生物学研究所，访问学者 2014.01-2015.01：美国康奈尔大学，分子遗传学系，访问学者 2013.01-至今：华东师范大学，生命科学学院，副研究员 2011.10-2012.12：华东师范大学，生命科学学院，助理研究员主持科研项目 2019年申请获得国家自然科学基金委面上项目：丝氨酸蛋白酶在家蚕精子功能调节中的作用研究（2020-2023，项目批准号：31970476） 2017年申请获得国家自然科学基金委面上项目：副性腺蛋白调节家蚕交配繁殖行为研究（2018-2021，项目批准号：31772517） 2013年申请获得国家自然科学基金委面上项目：泛素蛋白酶体途径在中华绒螯蟹精子顶体反应中的功能（2014-2017，项目批准号：41376157） 2012年申请获得国家自然科学基金委青年项目：ERKs和p38在中华绒螯蟹精子获能和顶体反应中的调节作用（2013-2015，项目批准号：31201974） 2018年申请获得上海市科委项目：CRISPR/Cas9基因编辑技术构建雌性卵泡发育缺陷模型（2018.05-2021.04，项目批准号：18140902200） 2012年申请获得上海市科委面上项目：中华绒螯蟹受精素基因及蛋白功能研究（项目批准号：12ZR1408900） 2012年申请获得教育部博士点基金新教师类项目：ERKs和p38在中华绒螯蟹精子顶体反应中的作用（课题编号：20120076120011）资助 2009年获得华东师范大学创新基金青年项目：资助开展精子功能方面的初步研究

研究领域

关注雄性生殖过程中精子成熟和修饰机理研究，同时对副性腺分泌蛋白在精子成熟和修饰过程中的作用感兴趣，借助高通量测序分析方法对精巢、副性腺组织的转录组、蛋白质组以及microRNA进行系统分析，从中发现与雄性生殖机理调控相关的信号途径和关键作用元件进行研究。目前着手深入的研究有以下几个方面的内容，第一个方面是副性腺蛋白调节家蚕交配繁殖行为研究，利用CRISPR/Cas9基因编辑技术构建Ser2基因突变体，获得雄性不育突变体；第二方面是关于MAPKs信号通路中与生殖调控相关的关键基因及蛋白功能研究，主要包括ERK、p38和JNK基因及蛋白在精子发生以及顶体反应过程中的调节作用研究；第三个方面是关于泛素蛋白酶体途径在精子顶体反应中的功能研究，包括泛素E3连接酶Culin4在精子发生和精巢发育过程中的功能研究以及泛素蛋白酶体途径参与的精子质量控制研究；另外我们还关注Hippo信号通路调节器官组织大小机制研究，以期从性腺发育角度调节昆虫交配繁殖行为。在此基础上，我们结合生殖生物学特点进行经济昆虫和害虫两个方面的开发研究，逐步建立益虫更益、害虫不害的转基因改造体系，利用昆虫资源更好的服务人类。

近期论文

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Jinjin Ding, Chunlai Cui, Guandong Wang, Ge Wei, Liang Bai, Yifei Li, Peilu Sun, Ling Dong, Zicheng Liu, Jiaqi Yun, Fang Li, Kai Li, Lin He, and Sibao Wang. Engineered gut symbiotic bacterium-mediated RNAi for effective control of Anopheles mosquito larvae. Microbiology Spectrum, 2023, 11(4): e01666-23. Xia Xu, Yao-Hui Wang, Zu-Lian Liu , Yong-Qiang Wang, Lin He, Kai Li, Yong-Ping Huang*. Disruption of egg-specific protein causes female sterility in Bombyx mori. Insect Science, 2022, 29(1):128-138. Xu Xia, Wang Yao hui, Bi Hong lun, Xu Jun, Liu Zu lian, Niu Chang Ying, He Lin*, Anthony A. James, Li Kai *, Huang Yong Ping *. Mutation of the seminal protease gene, serine protease 2, results in male sterility in diverse lepidopterans. Insect Biochemistry and Molecular Biology, 2020, 116: 103243. Xu Xia, Bi Hong Lun, Wang Yao Hui, Li Xiao Wei, Jun Xu, Liu Zu Lian, Lin He*，Li Kai*，Huang Yong Ping*. Disruption of the ovarian serine protease (Osp) gene causes female sterility in Bombyx mori and Spodoptera litura. Pest Management Science, 2020,76:1245-1255. Gao Yun xia, Bu Yun, He Lin*, Li Kai. A new species in the genus Dimorphacanthella (Collembola: Isotomidae) from Sichuan Province, Southwest China. Entomotaxonomia (2020) 42(1): 1–5. Bi Hong Lun, Xu Jun, He Lin, Zhang Yong, Li Kai, Huang Yong Ping.CRISPR/Cas9-mediated ebony knockout results in puparium melanism in Spodoptera litura. Insect Science. 2019 Dec; 26(6): 1011-1019. Xu Xia , Zhang Zhong Jie , Yang Yang, Huang Si Rui , Li Kai , He Lin* and Zhou Xu Guo. Genome editing reveals the function of Yorkie during the embryonic and early larval development in silkworm, Bombyx mori. Insect Molecular Biology, 2018,27(6):675-685. Yang Yang, Wang Yao Hui , Chen Xi'en, Tian Di, Xu Xia, Li Kai , Huang Yong Ping, He Lin*. CRISPR/Cas9 mediated Tyrosine hydroxylase knockout resulting in larval lethal in Agrotis ipsilon.Insect Science, 2018,25,1017-1024. Xu Xia, Bi Hong Lun, Zhang Zhong Jie , Yang Yang, Li Kai, Huang Yong Ping, Zhang Yong, He Lin*. BmHpo mutation induces smaller body size and late stage larval lethality in the silkworm, Bombyx mori. Insect Science, 2018,25,1006-1016. Akanksha Singh, Norene A. Buehner, He Lin, Kaitlyn J. Baranowski, Geoffrey D. Findlay and Mariana F. Wolfner*. Long-term interaction between Drosophila sperm and sex peptide is mediated by other seminal proteins that bind only transiently to sperm. Insect Biochemistry and Molecular Biology. 2018, 102: 43-51. Li Qing, Yang Hong Dan, He Lin, Wang Qun. Characterization of the Es-DDX52 involved in the spermatogonial mitosis and spermatid differentiation in Chinese mitten crab (Eriocheir sinensis）.Gene, 2018, 646: 106-119. Yang Hong Dan , Li Qing, Yu Ai Qing, He Lin*, Wang Qun. Molecular characterization and sub-celluiar distribution of JNK and JIP4 protein kinases in spermatogenesis and acrosome reaction of the Chinese mitten crab Eriocheir sinensis. Journal of Crustacean Biology, 00(0),1-11,2016. DOI: 10.1163/1937240X-00002468. Wang Yuan Li , Li Dan, Yang Hong Dan, He Lin, Sun Wen Juan, Duan Ze Lin, Wang Qun. The E3 ubiquitin ligase CRL4 regulates proliferation and progression through meiosis in Chinesemitten crab Eriocheir sinensis, Biology of Reproduction. 2016, 94(3), pp 1-14, 2016 3. Zhao Jin Cheng, Wang Yuan Li, Li Qing, Zhu Ming, Sun Wen Juan, Wu Tian Min, Wang Qun, He Lin*. Molecular Cloning and Characterization of p38 Gene in the Chinese Mitten Crab, Eriocheir sinensis. Aquaculture Research. 2016, 47: 1353-1363. He Lin Wang Yuan Li, Li Qing, Yang Hong Dan, Duan ZeLin, Wang Qun. Profiling microRNAs in the testis during sexual maturation stages in Eriocheir sinensis. Animal Reproduction Science. 2015 Nov. 162: 52-61. Li Qing, Xie Jing, He Lin, Wang Yang, Yang Hong Dan, Duan Ze Lin, Wang Qun. FOXL2 Down regulates Vitellogenin Expression at Mature Stage in Eriocheir sinensis. Biosci Rep. 2015Oct1.35(6) pii:BSR20150151. Li Qing, Liu Li Hua,Wang Yang, Xie Jing, He Lin*, Wang Qun. Characterization and expression analysis of serpins in the Chinese mittencrab Eriocheir sinensis.Gene.2016,575:632-640.doi:10.1016/j.gene.2015.09.033 He Lin, Li Qing, Liu Li Hua, Wang Yang, Xie Jing, Yang Hong Dan, Wang Qun. A Catalog of Proteins Expressed in the AG Secreted Fluid during the Mature Phase of the Chinese Mitten Crabs (Eriocheir sinensis). PLoS One. 2015 Aug 25;10(8):e0136266. doi: 10.1371/journal.pone.0136266. Wang Yuan Li ,Sun Wen Juan, He Lin, Li Qing, Wang Qun. Morphological alterations of all stages of spermatogenesis and acrosome reaction in Chinese mitten crab Eriocheir sinensis. Cell Tissue Res. 2015 May;360(2):401-12. Li Qing, Wang Yuan Li, Xie Jing, Sun Wen Juan, Zhu Ming, He Lin*, Wang Qun*. Characterization and expression of DDX6 during gametogenesis in the Chinese mitten crab Eriocheir sinensis. Genet. Mol. Res. 2015, 14 (2): 4420-4437. LiQing, Xie Jing, He Lin , Wang Yuan Li, Duan Ze Ling, Yang Hong Dan, Wang Qun. Identification of ADAM10 and ADAM17 with potential roles in the spermatogenesis of the Chinese mitten crab, Eriocheir sinensis. Gene. 2015 May 10;562(1):117-27. Sun Wen Juan, Zhu Ming, Wang Yuan Li, Li Qing, Yang Hong Dan, Duan Ze Lin, He Lin*,Wang Qun*. ERK Is Involved in the Process of Acrosome Reaction In Vitro of the Chinese Mitten Crab, Eriocheir sinensis. Mar Biotechnol (NY).2015, 17(3):305-16. Zhu Ming, Sun Wen Juan, Wang Yuan Li, Li Qing, Yang Hong Dan, Duan Ze Lin, He Lin*, Wang Qun*. P38 participates in spermatogenesis and acrosome reaction prior to fertilization in Chinese mitten crab Eriocheir sinensis. Gene. 2014 Nov 26. pii: S0378-1119(14)01327-4. doi: 10.1016/j.gene.2014.11.050. Wang Yuan Li, Li Qing, Xie Jing, Zhu Ming, Sun Wen Juan, He Lin*, Wang Qun*. Involvement of the single Cul4 gene of Chinese mitten crab Eriocheir sinensis in spermatogenesis. Gene. 2014 Feb 15;536(1):9-17. doi: 10.1016/j.gene.2013.11.099. He Lin, Jiang Hui, Cao Dan Dan, Liu Li Hua, Hu Song Nian, and Wang Qun. Comparative Transcriptome Analysis of the Accessory Sex Gland and Testis from the Chinese Mitten Crab (Eriocheir sinensis). PLoS One. 2013, 8: e53915. doi: 10.1371/journal.pone.0053915. He Lin, Xie Jing, Li Qing, Zhao Yun Long, Wang Ya Ping and Wang Qun. Isolation and Characterization of Polymorphic Microsatellite Loci in the Redclaw Crayfish, Cherax Quadricarinatus. J Aquac Res Development，2013, 4:162 doi:10.4172/2155-9546.1000162. Wang Juan, Fang Di An,Wang Yang, Wang Yuan Li, Cheng Lin, HeLin, and Wang Qun. Cathepsin A protein from accessory sex gland of the Chinese mitten crab (Eriocheir sinensis) plays a key role in spermatophore digestion. Journal of Insect Physiology.2013,,59 (2013) 953–960. DOI: 10.1016/j.jinsphys.2013.07.0032013. Cheng Lin, Jin Xing Kun, Li Wei Wei, Li Shuang, Guo Xiao Nv, Wang Juan, Gong Ya Nan , He Lin, Wang Qun . Fatty acid binding proteins FABP9 and FABP10 participate in antibacterial responses in Chinese mitten crab, Eriocheir sinensis.PLoS ONE 01/2013; 8(1): e54053. dio: 10.1371/journal.pone.0054053. He Lin, Wang Qun, Jin Xing Kun, Wang Ying, Chen Li Li, Liu Li Hua, Wang Yang. Transcriptome profiling of testis during sexual maturation stages in Eriocheir sinensis using Illumina sequencing. PLoS One. 2012,7(3):e33735. doi:10.1371/journal.pone.0033735. He Lin, Xie Yan Nan, Lu Wei, Wang Ying, Chen Li Li, Peter B Mather, Zhao Yun Long, Wang Ya Ping, Wang Qun. Genetic diversity in three redclaw crayfish (Cherax quadricarinatus) lines developed in culture in China. Aquaculture Research. 2012, 43, 75–83. Xie Yan nan, He Lin , Sun Jiang Ling, Chen Li Li, Zhao Yun Long, Wang Ya Ping and Wang Qun. 2010. Isolation and characterization of ?fteen microsatellite loci from the redclaw cray?sh, Cherax quadricarinatus. Aquat. Living Resour. 2010，23, 231–234. He Lin, Wang Si Bao, Miao Xue Xia, Wu Hong, Huang Yong Ping. Identification of necrophagous fly species using ISSR and SCAR markers, Forensic Science International，2007，168: 148–153. 何琳，王群。基于PCR的SSR标记分离方法综述，基因组学与应用生物学，2010，29（4）：775-782。何琳，刘丽花，汪洋。家蚕para 钠通道cDNA片段克隆与序列分析，应用昆虫学报，2011，48(3)：596-604。 Li Wei Wei, He Lin, Jin Xing Kun, Jiang Hui, Chen Li Li, Wang Ying, WangQun. Molecular cloning,characterization and expression analysis of cathepsin A gene in Chinese mitten crab, Eriocheir sinensis. Peptides, 2011,32(3): 518-525. Jin Xing Kun, Li Wei Wei, He Lin, Jiang Hui, Wang Ying, Chen Li Li, Wang Qun. Molecular cloning, characterization and expression analysis of two apoptosis genes, caspase and nm23, involved in the antibacterial response in Chinese mitten crab, Eriocheir sinensis. Fish and Shellfish Immunology, 2011, 30(1): 263-272. Hou Xue Li, Mao Qian, He Lin, Gong Ya Nan, Qu Di and Wang Qun. Accessory Sex Gland Proteins Affect Spermatophore Digestion Rate and Spermatozoa Acrosin Activity in Eriocheir sinensis. Journal of Crustacean Biology, 2010, 30(3): 435-440. Fang Di An, Wang Qun, He Lin, Wang Juan, Wang Yang. Characterization of heat shock protein 70 in the red claw crayfish (Cherax quadricarinatus): Evidence for its role in regulating spermatogenesis. Gene, 2012?492（1）：138-147. (2010 IF:2.266) Jiang Hui, Ren Fei, Sun Jiang Ling, He Lin, Li Wei Wei, Xie Yan Nan, Wang Qun. Molecular Cloning and Gene Expression Analysis of the Leptin Receptor in the Chinese Mitten Crab Eriocheir sinensis. PLoS ONE, 2010, 5(6): e11175. Lu Wei, Li WeiWei, Jin XinKun, He Lin, Jiang Hui, Wang Qun. (2011) Reproductive function of Selenoprotein M in Chinese mitten crabs (Eriocheir sinesis). Peptides. Fang Di An, Wang Qun, Wang Juan, He Lin , Liu Li Hua and Wang Ying. A novel DDX5 gene in the freshwater crayfish Cherax quadricarinatus is highly expressed during ontogenesis and spermatogenesis. Genetics and Molecular Research (2011) ,10(4):3963-3975. Mao Qian, Hou Xue Li, Xiao Ping, Ying Xian Ping, He Lin and Wang Qun. Identification of proteins of accessory sex gland from Eriocheir sinensis by two-dimensional electrophoresis and MALDI-TOF mass spectrometry. Invertebrate Reproduction and Development, 2009. 53(3): p. 145–153. Wang Qun, Wang Ying, Chen Li Li, He Lin, Li Wei Wei, Jiang Hui. Expression characteristics of the SUMOylation genes SUMO-1 and Ubc9in the developing testis and ovary of Chinese mitten crab, Eriocheir sinensis. GENE. 2012. 501(2):135-143. Wang Qun, Chen Li Li, Wang Ying, Li Wei Wei, He Lin, Jiang Hui. Expression characteristics of two ubiquitin/ribosomal fusion protein genes in the developing testis? accessory gonad and ovary of Chinese mitten crab? Eriocheir sinensis. Molecular Biology Reports. 2012. 39:6683-6692.