吴强 - 上海交通大学 - 系统生物医学研究院

个人简介

学习经历 1990年和1993年分别获得复旦大学学士和硕士。1998年获美国纽约州立大学石溪分校细胞发育生物学博士。1999年-2001年，哈佛大学博士后，Damon Runyon Research Fellow。工作经历上海交通大学特聘教授，系统生物医学研究院和上海市肿瘤研究所研究员，博士生导师。系统生物医学教育部重点实验室常务副主任，比较生物医学研究中心主任，科技部重大科学研究计划项目（973）首席科学家，享受国务院政府特殊津贴专家，上海市优秀学术带头人。2008年回国前任犹他大学助理教授。

研究领域

We are interested in the regulation and function of the protocadherin and Ugt clusters during brain development as well as in brain diseases. To this end, we have developed novel methods of CRISPR DNA-fragment editing or chromosomal rearrangements to investigate mechanisms of three-dimensional (3D) genome folding. Finally, we are interested in the role of genome architectural protein CTCF in higher-order chromatin structure and its dynamic regulation.

近期论文

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Shou J., Li J., Liu Y., Wu Q. (2018) Precise and predictable CRISPR chromosomal rearrangements reveal principles of Cas9-mediated nucleotide insertion. Molecular Cell. 71, 498-509. Epub 2018 Jul 19. Fan L., Lu Y., Shen X., Shao H., Suo L., Wu Q. (2018) Alpha protocadherins and Pyk2 kinase regulate cortical neuron migration and cytoskeletal dynamics via Rac1 GTPase and WAVE complex in mice. eLife. 7. pii: e35242. doi: 10.7554/eLife.35242. Highlighted in eLife Digest. Xu D., Ma, R, Zhang, J, Liu Z., Wu B., Peng J., Zhai Y., Gong Q., Shi Y., Wu J., Wu Q., Zhang Z., Ruan K. (2018) Dynamic nature of CTCF tandem 11 Zinc fingers in multivalent recognition of DNA as revealed by NMR spectroscopy. J. Phys. Chem. Lett. 9: 4020-4028. doi: 10.1021/acs.jpclett.8b01440. Epub 2018 Jul 6. Shen X.L., Lu Y.C., Jia Z.L., Wu Q. (2018) N-WASP regulates cortical neuron migration through its polyPro and VCA domains. Heriditas (Yi Chuan). 40: 390-401. doi: 10.16288/j.yczz.18-066. Yin, M., Wang, J., Wang, M., Li, X., Zhang, M., Wu, Q., Wang, Y. (2017) Molecular mechanism of directional CTCF recognition of a diverse range of genomic sites. Cell Research 27: 1365-1377. Chen W.V., Nwakeze C.L., Denny C.A., O'Keeffe S., Rieger M.A., Mountoufaris G., Kirner A., Dougherty J.D., Hen R., Wu Q., Maniatis T. (2017) Pcdhαc2 is required for axonal tiling and assembly of serotonergic circuitries in mice. Science. 356: 406-411. doi: 10.1126/science.aal3231. Research Highlighted in Perspective in Science, 356: 376-378. Chen, D., Zuo, Y., Zhang, X., Ye, Y., Bao, X., Huang, H., Tepakhan, W., Wang, L., Ju, J., Chen, G., Zheng, M., Liu, D., Huang, S., Zong, L., Li, C., Chen, Y., Zheng, C., Shi, L., Zhao, Q., Wu, Q., Fucharoen, S., Zhao, C., and Xu, X. (2017). A Genetic Variant Ameliorates beta-Thalassemia Severity by Epigenetic-Mediated Elevation of Human Fetal Hemoglobin Expression. Am J Hum Genet 101: 130-138. Zhai Y., Xu Q., Guo Y., Wu Q. (2016). Characterizationof a cluster of CTCF-binding sites in a protocadherin regulatory region. Heriditas 38: 323-336. Burgess, S., Cheng, L., Gu, F., Huang, J., Huang, Z., Lin, S., Li, J., Li, W., Qin, W., Sun, Y., Songyang, Z., Wei, W., Wu, Q., Wang, H., Wang, X., Xiong, J.W., Xi, J., Yang, H., Zhou, B., and Zhang, B. (2016). Questions about NgAgo. Protein & cell 7, 913-915. Guo, Y., Q. Xu, D. Canzio, J. Shou, J. Li, D.U. Gorkin, I. Jung, H. Wu, Y. Zhai, Y. Tang, Y. Lu, Y. Wu, Z. Jia, W. Li, M.Q. Zhang, B. Ren, A.R. Krainer, T. Maniatis, Wu Q. (2015). CRISPR inversion of CTCF sites alters genome topology and enhancer/promoter function. Cell 162: 900-910. doi:10.1016/j.cell.2015.07.038. CELL Hottest Article. Research Highlighted in Cell, 162, 703–705; Nature Reviews Molecular Cell Biology 16, 578–579. Li J, Shou J，Guo Y, Tang Y, Wu Y, Jia Z, Zhai Y, Chen Z, Xu Q, Wu, Q. (2015). Efficient inversions and duplications of mammalian regulatory DNA elements and gene clusters by CRISPR/Cas9. J. Mol. Cell Biol. 7: 284-298. Cover Article. Jia Z, Guo Y, Tang Y, Xu Q, Li B, Wu Q. (2014) Regulation of the protocadherin Celsr3 gene and its role in globus pallidus development and connectivity. Mol Cell Biol. 34: 3895-3910. Wang Y., Huang H., Wu Q. (2014) Characterization of the zebrafish UGT repertoire reveals a new class of drug-metabolizing UDP glucuronosyltransferases. Mol. Pharmacol. 86: 62-75. Guo Y., Monahan K., Wu H., Varley KE., Gertz J., Li W., Myers RM., Maniatis T., Wu Q. (2012) CTCF/Cohesin-mediated DNA Looping is required for protocadherin alpha promoter choice. Proc Natl Acad Sci USA 109: 21081-21086. Suo L., Lu H., Ying G., Capecchi M.R., Wu, Q. (2012) Protocadherin clusters and cell adhesion kinase regulate dendrite complexity through Rho GTPase. J Mol Cell Biol 4: 362-376. Cover Article Yang J., Cai L., Huang H., Liu B., Wu, Q. (2012) Genetic variations and haplotype diversity of the UGT1 gene cluster in the Chinese population. PLoS One 7: e33988. Wu, Q. and Maniatis, T. (1999) A striking organization of a large family of human neural cadherin-like cell adhesion genes. Cell 97: 779-790. Research highlighted in Cell, 98: 133–136; Neuron, 23: 427–430; Current Biology 9: R651–R654; Nature neuroscience 2: 686-687. Wu, Q. and Krainer, A.R. (1996) U1-mediated exon definition interactions between AT-AC and GT-AG introns. Science 274: 1005-1008.