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个人简介

新疆大学学士,中国科学院上海药物研究所硕士,复旦大学分子遗传与生物化学博士,美国得克萨斯州大学奥斯汀分校博士后,美国密西根州立大学访问学者。现任上海交通大学助理研究员。主要从事结构酶学研究,通过晶体结构生物学及联合生化、生物物理等技术手段解析代谢及合成途径中关键酶的结构,揭示酶分子的催化、稳定性机制,一方面以此为基础对生物技术及医学、工业用酶进行性质优化再设计;另一方面致力于基于结构的代谢调控酶的小分子抑制剂设计合成 。

研究领域

新功能酶的克隆、表征与改造 酶的结构解析及分子机制分析 酶分子进化,酶的小分子抑制剂设计合成

近期论文

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Md Anarul Hoque#, Yong Zhang#, Liuqing Chen, Guangyu Yang, Mst Afroza Khatun, Haifeng Chen,Liu Hao, Yan Feng*, Stepwise Loop Insertion, Strategy for Active Site emodeling to Generate Novel Enzyme Functions. ACS Chem. Biol., 2017, 32(27)132-137(# Contributed equally) Yong Zhang, Jiao An, Guangyu Yang, Xiaofei Zhang, Yuan Xie, Liuqing Chen, Yan Feng*. Structure features of GH10 xylanase from Caldicellulosiruptor bescii: implication for its thermophilic adaption and substrate binding preference. Acata Bioch. Bioph. Sin., 2016, 56(7). 56-67 Yong Zhang; Jian Bao; Xinxian Deng; Wan He; Jiajun Fan; Lei Fu*. Synthesis, biological evaluation and molecular docking of 2-phenyl- benzo[d] oxazole- 7-carboxamide derivatives as potential Staphylococcus aureus sortaseA inhibitors. Bioorg Med Chm Lett., 2016, 34(3). 21-28 Wan He#, Yong Zhang#, Jian Bao, Xinxian Deng, Jennifer Batara,Shawn Casey, Faqin Jiang*, Lei Fu*, Synthesis, biological evaluation and molecular docking analysis of 2-phenyl-benzofuran-3-carboxamide as a potential inhibitors of Sortase A. Bioorg & Med Chem. , 2016, 217(25)1341~1351 (# Contributed equally) Yong Zhang, Mengmeng Zhang and Yan Zhang*. Crystal structure of Ssu72, an essential eukaryotic phosphatase specific for the C-terminal domain of RNA polymerase II, in complex with a transition state analogue. Biochem J., 2011, 434(3):435-44. Yong Zhang, Chaoneng Ji, Zhenxing Yang. Chaoneng Ji*. A Moderately thermostable alkaline phosphatase from Geobacillus thermodenitrificans T2: Cloning, expressionand biochemical characterization, Appl. Biochem. Biotechnol., 2008, 151 (1): 81-92 Yong Zhang, Zhenxing Yang, Xiaodong Huang. et al Cloning Expression and Characterization of a thermostable 1 PAP2L2, a new member of type 2 phosphatidic acid phosphatase family from Geobacillus toebii T-85, Biosci. Biotechnol. Biochem,, 2008, 72(12):3134-4 Ang Li, Yong Zhang*, Che Wang, Geng Wu*,Zhenchun Wang, Purification, molecular cloning, and antimicrobial activity of peptides from the skin secretion of the black-spotted frog, Rana nigromaculata. World J. Microbiol. Biotechnol., 2013, 32(2): 401-19 (*Co-corresponding author) Seema Irani, Joshua Mayfield, Yong Zhang, Wendy L. Matthews and Yan Jessie Zhang*,Structure of S. cerevisiae Rtr1 reveals an atypical phosphatase with novel reaction mechanism, Sci. Signal., 2016, 9(417) Xiaofei Zhang, Yong Zhang, Guangyu Yang, Yuan Xie, Lishi Xu, Jiao An, Li Cui, Yan Feng*, Modulation of the thermostability and substrate specificity ofCandida rugosa lipase1 by altering the acyl-binding residue Gly414 at the α-helix-connecting bend, Enzyme Microb. Technol. 2015, 82(22):34-41 Jiao An, Yuan Xie, Yong Zhang, Dongsheng Tian, Shuhao Wang, Guangyu Yang, Yan Feng*, Characterization of a thermostable, specific GH10 xylanase from Caldicellulosiruptor bescii with high catalytic activity, J. Mol. Catal., B Enzym. 2015, 117, 13-20 Yuan Xie, Jiao An, Guangyu Yang, Geng Wu, Yong Zhang, Li Cui and Yan Feng*, Enhanced enzyme kinetic stability by increasing rigidity within the active Site, J. Biol. Chem., 2014, 15. 22-28 Zhenxing Yang, Yong Zhang, Rui Qiu, Jing Huang and Chaoneng Ji*, Crystallization and Preliminary X-Ray Diffraction Analysis of a thermostable GDSL family esterase, EstL5, from Geobacilus thermodenitrificans T2. Acta Cryst., 2013, 69, 776-778 Zhenxing Yang, Yong Zhang, Tiantian Shen, Yi Xie, Yumin Mao*, and Chaoneng Ji*. Expression and biochemical characterization of a novel moderately thermostable GDSL family esterase from Geobacillus thermodenitrificans T2. J Biosci Bioeng. 2013, 115(2):133-137 Jingbao Liu, Faqin Jiang, Yong Zhang, Jingjing Liu, Wenlu Liu, Lei Fu*. Design, Synthesis, and evaluation of 2-substituted ethenesulfonic acid ester derivatives as protein tyrosine phosphatase 1B inhibitors. E. J. Med. Chem., 2012, 57(5), 10-20 Xiangwei Fei, Yong Zhang, Xin Gu, Rui Qiu, Yumin Mao,Chaoneng Ji*. Crystallization and preliminary X-Ray analysis of the splice variant of human ankyrin repeat and suppressor of cytokine signaling box protein 9 (hASB9-2). Protein Sci., 2008,15(6):647-9. Hui Qian, Chaoneng Ji, Yong Zhang, Mi Yan, Yaqiong Sun, Yi Xie, Yumin Mao*. Expression and characterization of HSPC129, a RNA polymerase II C-terminal domain phosphatase , Mol. Cell Biochem., 2007, 303:183–188

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