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个人简介

中国科学院微生物研究所获生化与分子生物学博士学位。2007年起任职于上海交通大学微生物代谢教育部重点实验室。2011-2013,加州大学伯克利分校,唐氏学者。

研究领域

冷冻电镜技术解析蛋白结构: 蛋白分子机器的表达和纯化 液态乙烷中蛋白质冷冻样品的制备 冷冻电镜操作与观察 计算解析蛋白三维结构模型 天然产物合成机器装配的候选研究主题: 常用的抗生素如红霉素、青霉素的天然产物的合成机器。 洛伐他丁类的合成机器:

近期论文

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Pu T, Liang J, Mei Z, Yang Y, Wang J, Zhang W, Liang W, Zhou X, Deng Z*, Wang Z*. Phosphorothioated DNA is shielded from oxidative damage. Applied and Environmental Microbiology. 2019 Feb 8. pii: AEM.00104-19. doi: 10.1128/AEM.00104-19. [Epub ahead of print] Wu H, Liang J, Gou L, Wu Q, Liang W, Zhou X, Bruce IJ, Deng Z*, Wang Z*. Recycling of Overactivated Acyls by a Type II Thioesterase during Calcimycin Biosynthesis in Streptomyces chartreusis NRRL 3882. Applied and Environmental Microbiology. 2018 May 31;84(12). e00587-18. Wu H, Liang J, Wang J, Liang W, Gou L, Wu Q, Zhou X, Bruce IJ, Deng Z*, Wang Z*. Cezomycin Is Activated by CalC to Its Ester Form for Further Biosynthesis Steps in the Production of Calcimycin in Streptomyces chartreusis NRRL 3882. Applied and Environmental Microbiology. 2018 May 31;84(12). e00586-18. Zhao Y, Li L, Zheng G, Jiang W, Deng Z, Wang Z*, Lu Y*. CRISPR/dCas9-Mediated Multiplex Gene Repression in Streptomyces. Biotechnology Journal. 2018 Sep;13(9): e1800121. Dai D, Pu T, Liang J, Wang Z*,Tang A*. Regulation of dndB Gene Expression in Streptomyces lividans. Frontiers in Microbiology. 2018 Oct 8; 9:2387. Yang Y, Xu G, Liang J, He Y, Xiong L, Li H, Bartlett D, Deng Z, Wang Z*, Xiao X*. DNA Backbone Sulfur-Modification Expands Microbial Growth Range under Multiple Stresses by its anti-oxidation function. Scientific Reports. 2017 Jun 14;7(1):3516. Gou L, Han T*, Wang X, Ge J, Liu W, Hu F, Wang Z*. A Novel TetR Family Transcriptional Regulator, CalR3, Negatively Controls Calcimycin Biosynthesis in Streptomyces chartreusis NRRL 3882. Frontiers in Microbiology. 2017 Nov 29; 8:2371. Dai D, Du A, Xiong K, Pu T, Zhou X, Deng Z, Liang J*, He X*, Wang Z*. DNA Phosphorothioate Modification Plays a Role in Peroxides Resistance in Streptomyces lividans. Frontiers in Microbiology. 2016 Aug 31; 7:1380. Li F, Liang J, Wang W, Zhou X, Deng Z*, Wang Z*. Two nucleoside receptors from Streptomyces coelicolor: expression of the genes and characterization of the recombinant proteins. Protein Expression and Purification. 2015 May; 109:40-6. Li F, Liang J, Wang W, Zhou X, Deng Z*, Wang Z*. Analysis of Streptomyces coelicolor membrane proteome using two-dimensional native/native and native/sodium dodecyl sulfate gel electrophoresis. Analytical biochemistry. 2014 Aug 18; 465C:148-155. Gou L, Wu Q, Lin S, Li X, Liang J, Zhou X, An D*, Deng Z*, Wang Z*. Mutasynthesis of Pyrrole Spiroketal Compound by Using Calcimycin 3-Hydroxy Anthranilic Acid Biosynthetic Mutant. Applied Microbiology and Biotechnology. 2013 Sep;97(18):8183-91. Wu Q, Gou L, Lin S, Liang J, Yin J, Zhou X, Bai L, An D, Deng Z*, Wang Z*. Characterization of the N-methyltransferase CalM involved in calcimycin biosynthesis by Streptomyces chartreusis NRRL 3882. Biochimie, 2013 Jul.95 (7):1487-93. Xie X, Liang J, Pu T, Xu F, Yao F, Yang Y, Zhao Y, You D, Zhou X, Deng Z*, Wang Z*. Phosphorothioate DNA as an antioxidant in bacteria. Nucleic Acids Research. 2012 Oct; 40(18):9115-24. An X, Xiong W, Yang Y, Li F, Zhou X, Wang Z, Deng Z*, Liang J*. A novel target of IscS in Escherichia coli: participating in DNA phosphorothioation. PLoS One. 2012; 7(12):e51265. Wu Q, Liang J, Lin S, Zhou X, Bai L*, Deng Z and Wang Z*. Characterization of the Pyrrole Polyether Antibiotic Calcimycin (A23187) Biosynthetic Gene Cluster from Streptomyces chartreusis NRRL 3882. Antimicrobial Agents and Chemotherapy (AAC). 2011 Mar; 55(3):974-82. Liang J, Niu Q, Xu X, Luo Y, Zhou X, Deng Z*, Wang Z*. Effective elimination of nucleic acids from bacterial protein samples for optimized blue native polyacrylamide gel electrophoresis. Electrophoresis. 2009 Jul; 30(14):2454-9. Xu T*, Liang J*, Chen S, Wang L, He X, You D, Wang Z, Li A, Xu Z, Zhou X, Deng Z. (2009) DNA phosphorothioation in Streptomyces lividans: Mutational analysis of the dnd locus. BMC Microbiol. 2009 Feb 20; 9(1):41. Xu X#,Wang Z#,Fan K,Wang S,Jia C,Han H,Ramalingam E,Yang K. Localization of the ActIII actinorhodin polyketide ketoreductase to the cell wall. FEMS Microbiology Letters. 2008 Oct; 287(1):15-21. Liang J#, Wang Z#, He X, Li J, Zhou X,Deng Z. DNA modification by sulfur: analysis of the sequence recognition specificity surrounding the modification sites. Nucleic Acids Research. 2007 Apr; 35(9):2944-54. Wang Z, Xu X, Fan K, Jia C, Yang K. Sample preparation for two-dimensional blue native/SDS polyacrylamide gel electrophoresis in the identification of Streptomyces coelicolor cytoplasmic proteincomplexes. Journal of Biochemical and Biophysical Methods. 2007 Jun 10; 70(4):565-72.

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