王晓健 - 浙江大学

个人简介

1. 2001.9-2006.6年：师从曹雪涛院士, 主要从事细胞免疫学及免疫新分子的克隆、表达及功能等的研究。其中负责研究的新型磷脂酰乙醇胺结合蛋白，新型1型磷酸酶抑制因子的克隆和功能研究已获的重要结果，以第一作者发表与国际权威杂志（J Biol Chem 2004;Clin cancer Res 2005; J Biol Chem 2007;J Biol Chem 2008等杂志）；并以第一申请人申请国家发明专利3项，国际专利2项，已获得授权两项（ZJ 02136556.3；ZL 02136524.5）。 2. 2007.5-2009.11：在美国Yale 大学免疫系Warren D. Shlomchik实验室进修，主要从事移植物抗宿主疾病（GVHD）的发病机制研究。Shlomchik教授实验室在研究GVHD和GVL的领域一直处于世界领先水平，其相关成果发表在Science, Nat Med, Immunity等高水平杂志。本人在Shlomchik实验室访问期间，主要研究抗原递呈细胞（APC）及次要相容性组织抗原（mHAgs）在GVHD 发生，发展中的作用。发现在MHC不相合诱导的GVHD疾病中，如果供者APC缺失MHCII, GVHD的严重性显著下降，而如果供者和受者之间无次要相容性组织抗原差异，供者APC不能促进GVHD的发展，提示供者APC通过间接递呈受者的mHAgs，促进MHC 不相合诱导的GVHD发生发展。同时，本人运用基因敲除，注射特异性抗体等手段分别删除各种APC，研究各种受者APC在GVHD发生发展的作用,发现GVHD的发生不需要受者B细胞。部分相关结果发表与Biol Blood Marrow Transplant 2010. BLOOD 2011。 3．2009.11-至今：回国后在浙江大学医学院建立独立的实验室，致力于肿瘤免疫和天然免疫的调控机制研究，发现hPEBP4具有促进肿瘤转移的功能，并揭示了其分子机制，研究结果以通讯作者发表于Plos One 2013、Oncogene 2013；证明天然免疫重要调控蛋白TAK1活化必须的新型磷酸化位点及调控机制，部分研究结果以通讯作者发表于 J Immunol 2014.

研究领域

免疫新分子的克隆和功能研究，肿瘤免疫，固有免疫与感染免疫。

近期论文

查看导师新发文章（温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

1. Gu M , Ouyang C, Lin W, Zhang T, Cao X, Xia Z, Wang X*. hosphatase holoenzyme PP1/GADD34 negatively regulates TLR response by inhibiting TAK1 serine 412 phosphorylation. J Immunol. 2014 192. 192(6):2846-56 (*通讯作者) 2. Li H, Huang F, Fan L, Jiang Y, Wang X, Li J, Wang Q, Cao X, Wang X*. Phosphatidylethanolamine-binding protein 4 is associated with breast cancer metastasis through Src-mediated Akt tyrosine phosphorylation. Oncogene. 2013 Nov 25. doi: 10.1038/onc.2013.408. [Epub ahead of print] PMID: 24276246. (*通讯作者) 3. Wang K, Jiang Y, Zheng W, Liu Z, Li H, Lou J, Gu M, Wang X*. Silencing of human phosphatidylethanolamine-binding protein 4 enhances rituximab-induced death and chemosensitization in B-cell lymphoma. PLoS One. 2013;8(2):e56829. (*通讯作者) 4. Wang X, Li H, Matte-Martone C, Cui W, Roopenian D, Shlomchik WD.. Mechanisms of antigen presentation to T cells in murine graft-vs-host disease: crosspresentation and the appearance of cross-presentation. Blood.2011;118:6426-37. 5. Matte-Martone C1, Wang X, (shared first author), Anderson B, Jain D, McNiff J, Shlomchik MJ, Shlomchik WD. Recipient B Cells Are Not Required For GVHD Induction. Biol Blood Marrow Transplant. 2010;16:1222-30. 6. Wang X, Liu B, Li N, Li H, Qiu J, Zhang Y, Cao X. et al. IPP5, a novel protein inhibitor of protein phosphatase 1, promotes G1/S progression in a Thr-40-dependent manner. J Biol Chem. 2008; 283:12076-84. 7. Wang X, Li N, Li H, Liu B, Qiu J, Chen T, Cao X. Silencing of human phosphatidylethanolamine-binding protein 4 sensitizes breast cancer cells to TNFα-induced apoptosis and cell growth arrest. Clin Cancer Res. 2005;11:7545-53. 8. Wang X, Li N, Liu B, Sun H, Chen T, Li H,Wan T, Cao X. A novel human phosphatidylethanolamine-binding protein resists TNF-induced apoptosis by inhibiting MAPK pathway activation and phosphatidylethanolamine externalization. J Biol Chem. 2004;279:45855-64. 9. Li H, Wang X, (shared first author) Li N, Qiu J, Zhang Y, Cao X. hPEBP4 resists TRAIL-induced apoptosis of human prostate cancer cells by activating Akt and deactivating ERK1/2 pathways. J Biol Chem. 2007;282:4943-50. 10. Zhang Y, Wang X, (shared first author), Xiang Z, Li H, Qiu J,Li N, Cao X, Wang J. Promotion of cellular migration and apoptosis resistance by a mouse eye-specific phosphatidylethanolamine-binding protein. Int J Mol Med. 2007;19:55-63.. 11. Liu Y, Lai L, Chen Q, Song Y, Xu S, Ma F, Wang X, Wang Q.MicroRNA-494 is required for the accumulation and functions of tumor-expanded myeloid-derived suppressor cells via targeting of PTEN. J Immunol. 2012;188(11):5500-10. 12. Qiu J, Wang X, Guo X, Zhao C, Wu X, Zhang Y. Toll-like receptor 9 agonist inhibits ERalpha-mediated transactivation by activating NF-kappaB in breast cancer cell lines. Oncol Rep. 2009 ;22(4):935-41. 13. Wang Q, Li N, Wang X et al. Membrane protein hMYADM preferentially expressed in myeloid cells is up-regulated during differentiation of stem cells and myeloid leukemia cells. Life Sci. 2007 ;80:420-9. 14. Xiu F, Cai Z, Yang Y, Wang X et al. Surface anchorage of superantigen SEA promotes induction of specific antitumor immune response by tumor-derived exosomes.J Mol Med. 2007;85(5):511-21. 15. Yan X, Xiu F, An H, Wang X, Wang J, Cao X . Fever range temperature promotes TLR4 expression and signaling in dendritic cells. Life Sci. 2007;80(4):307-13. 16. Sun H, Li N, Wang X, Chen T,Shi L, Zhang L, Wan T, Wang J,Cao X. Molecular Cloning and Characterization of a Novel Muscle Adenylosuccinate Synthetase, AdSSL1, from Human Bone Marrow Stromal Cells. Molecular and Cellular Biochemistry 2005 Jan;269(1-2):85-94. 17. He X, Wang J, Guo Z, Liu Q, Chen T, Wang X, Cao X. Requirement for ERK activation in sinomenine-induced apoptosis of macrophages. Immunol Lett. 2005 Apr 15;98(1):91-6. Epub 2004 Nov 25. 18. Wang B, Li N, Sui L, Wu Y, Wang X, Wang Q, Xia D, Wan T, Cao X.HuBMSC-MCP, a novel member of mitochondrial carrier superfamily, enhances dendritic cell endocytosis. Biochem Biophys Res Commun. 2004 Jan 30;314(1):292-300. 19. Sun H, Li N, Wang X, Liu S, Chen T, Zhang L, Wan T, Cao X. Molecular cloning and characterization of a novel cystatin-like molecule, CLM, from human bone marrow stromal cells. Biochem Biophys Res Commun. 2003 Jan 31;301(1):176-82.