研究领域

Analytical Chemistry Bioanalytical Chemistry Biophysical Chemistry Chemical Biology Materials Chemistry Nano Chemistry Optics and Imaging Physical Chemistry

Biological Applications of Single-Molecule Imaging and Nanophotonics Research in our group seeks to maximize the impact of single-molecule fluorescence and nanophotonics by applying them to investigations of live cells. The extension of sophisticated nanoscale optoelectronic tools, techniques and materials to biological systems will enable fundamental discoveries, broaden our understanding of key biological processes, and assist in the development of novel therapeutics. Undertaking such an endeavor at the crossroads of chemistry, biology and engineering requires the development of sensitive experimental methods and careful, quantitative analysis procedures. Super-resolution techniques based on single-molecule optical microscopy can reach nanometer-scale accuracy. These non-invasive, non-perturbative methods are ideal for investigating biological specimens, and we focus on improving these methods and applying them to physiologically relevant problems. Because of their small size and lack of subcellular compartments, the cell biology of bacteria is particularly interesting for super-resolution imaging. We have developed novel methods for single-molecule investigations and have been applying them to three prokaryotic systems: membrane-bound transcription activation in Vibrio cholerae, carbohydrate catabolism in Bacteroides thetaiotaomicron, and DNA mismatch repair in Bacillus subtilis. In order to treat these and other problems, we seek to improve current single-molecule imaging techniques. One major thrust is to combine single-molecule fluorescence imaging with plasmon-enhanced fluorescence. In this work, we explore the fundamental optical properties of noble metal nanoparticles, and use the enhanced local field about resonantly excited nanoparticles to increase fluorescence intensity and photostability in bio-imaging applications.

近期论文

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E. Wertz, B.P. Isaacoff, J.E. Donehue and JSB, “Single-molecule fluorescence unravels the coupling of light to a plasmonic nano-antenna,” submitted (2014). Y. Liao, J.W. Schroeder, L.A. Simmons and JSB, “Mismatch detection by MutS strictly depends on coupling to DNA replication,” submitted (2014). B.L. Haas, J.S. Mason, V.J. DiRita and JSB, “Single-molecule tracking in live Vibrio cholerae reveals that ToxR recruits the membrane-bound virulence regulator TcpP to the toxT promoter,” Molecular Microbiology, in press (2014). DOI H.H. Tuson and JSB, “Unveiling the Inner Workings of Live Bacteria Using Super-Resolution Microscopy,” Analytical Chemistry, 87 42-63 (2015). DOI T. Hu, B.P. Isaacoff, Y. Zhou, J. Zhu, X. Li, Z. Wang, S. Liu, JSB* and N.A. Kotov*, “Self-Organization of Plasmonic and Excitonic Nanoparticles into Resonant Chiral Supraparticle Assemblies,” Nano Letters, 14 6799-6810 (2014). DOI K.S. Karunatilaka, E.A. Cameron, E.C. Martens, N.M. Koropatkin and JSB, “Super-Resolution Imaging Captures Carbohydrate Utilization Dynamics in Human Gut Symbionts,” mBio, 5 e02172-14 (2014). DOI B.L. Haas, J.S. Mason, V.J. DiRita and JSB, “Imaging live cells at the nanometer-scale with single-molecule microscopy: Obstacles and achievements in experimental optimization for microbiology,” Molecules, 19 12116-12149 (2014). DOI J.E. Donehue, E. Wertz, C.N. Talicska and JSB, “Plasmon-Enhanced Brightness and Photostability from Single Fluorescent Proteins Coupled to Gold Nanorods,” Journal of Physical Chemistry C, 118 15027-15035 (2014). DOI D.J. Rowland and JSB, “Top-Hat and Asymmetric Gaussian Based Fitting Functions for Quantifying Directional Single-Molecule Motion,” ChemPhysChem, 15 712-720 (2014). DOI JSB, “Intracellular dynamics of bacterial proteins are revealed by super-resolution microscopy,” Biophysics Journal, 105 1547-1548 (2013). DOI J.E. Donehue, B.L. Haas, E. Wertz, C.N. Talicska and JSB, “Plasmon-enhanced emission from single fluorescent proteins,” Proceedings of SPIE, 8597 85970J (2013). DOI E. Wertz, J.E. Donehue, C. Hayes and JSB, “Plasmon-enhanced fluorescence intensities and rates permit super-resolution imaging of enhanced local fields,” Proceedings of SPIE, 8590 85900U (2013). DOI K.S. Karunatilaka, B.R. Coupland, E.A. Cameron, E.C. Martens, N.M. Koropatkin and JSB, “Single-Molecule Imaging Can Be Achieved in Live Obligate Anaerobic Bacteria,” Proceedings of SPIE, 8590 85900K (2013). DOI M.-C. Cheng, A. T. Leske, T. Matsuoka, B. C. Kim, J.-S. Lee, M. A. Burns, S. Takayama and JSB, “Super-Resolution Imaging of PDMS Nanochannels by Single-Molecule Micelle-Assisted Blink Microscopy,” Journal of Physical Chemistry B, 117 4406-4411 (2013). DOI Y. Liao, S.K. Yang, K. Koh, A.J. Matzger and JSB, “Heterogeneous Single-Molecule Diffusion in One- Two- and Three-Dimensional Microporous Coordination Polymers: Directional, Trapped and Immobile Guests,” Nano Letters, 12 3080 (2012). DOI JSB, “Extending the tools of single-molecule fluorescence imaging to real problems in microbiology,” Molecular Microbiology, 85 1 (2012). DOI JSB, “Moving toward the future of single-molecule-based super-resolution imaging,” Biopolymers, 95 287 (2011). DOI JSB, “Live-Cell Single-Molecule Imaging,” Encyclopedia of Biophysics, vol. 1, G.C.K. Roberts (ed.), Springer (Heidelberg, 2013). ISBN 978-3-642-16711-9 JSB, “Photoactivated Localization Microscopy (PALM),” Encyclopedia of Biophysics, vol. 1, G.C.K. Roberts (ed.), Springer (Heidelberg, 2013). ISBN 978-3-642-16711-9 JSB, E.D. Goley, L. Shapiro and W.E. Moerner, “Three-Dimensional Super-Resolution Imaging of the Midplane Protein FtsZ in Live Caulobacter crescentus Cells Using Astigmatism,” ChemPhysChem, 13 1007 (2012). DOI