个人简介

B.Sc. Victoria; Ph.D. CityU H.K.

研究领域

Analytical Chemistry, Physical chemistry

Biophysicals and fundamentals in Mass Spectrometry: We employ mass spectrometry experiments and computational models in our laboratory to study the pathways towards and energetic of the formation of peptide radical ions. Peptides radicals were believed to play important roles in various biological processes, including aging and Parkinson’s disease, but are understood only poorly at present. Through our investigations we seek a better understanding of the generation of peptide radicals as well as new insight into the fundamental aspect of the chemistry involved, such as the mechanisms of gas phase ion fragmentations. . Energetic, structures and mechanisms studies of biological molecules Bioanalyticals and next generation of Mass Spectrometry Instrumentation: Interdisciplinary research in this group focuses on the development of new instrumentation and techniques in liquid chromatography mass spectrometry (LC-MS) for the biomolecules applications. We are continually interested in modify existing mass spectrometric hardware in collaboration with mass spectrometer manufacturer that may explore the new ways of glycomics and metabolomics analysis. Sensitive, efficient and automated mass spectrometric methodologies will be developed for proteomics, glycomics and metabolomics analysis. A B C Mass Spectrometry-Based Proteomics, Glycomics and Metabolomics: We are actively engaged in the application of mass spectrometry to proteomics and glycomics. Using our analytical tools, we are identifying and characterizing glycoproteins that participate in several novel and important cellular functions. With our collaborators, we are studying metabolomics with the goal of identifying metabolites of great botanical and biomedical interest. A: Immunohistochemical analysis of endothelial cells B: A novel metabolic gene in sorghum and zebrafish embryos; C: MRI image of DDD (Degenerative Disc Disease) Analysis of Toxic Organic Substances in Food Matrixes The presence of toxic organic substances such as pesticides, naturally occur toxins, drug residues and artificial chemicals additives in food may cause severe short term or chronic health problems. There are huge un-matched demands in providing more sensitive and efficient analysis for food safety testing to better protect the general public and to reduce the trade barriers in food items over the world. We are interested in coupling Multi-Dimension Liquid Chromatography to mass spectrometry with dual ESI and APCI interfaces in attempts to tackle some of the challenging issues in food analysis such as matrix interferences, insufficient sensitivity for low level toxic substances and related metabolites, and lengthy sample preparation prior to LC-MS-MS analysis.

近期论文

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Zhang Z, Li G, Szeto SSW, Chong C, Quan Q, Huang C, Cui W, Guo B, Wang Y, Han Y, Siu MKW, Lee SMY, Chu IK. ""Examining the neuroprotective effects of protocatechuic acid and chrysin on in vitro and in vivo models of Parkinson's disease."" Free Radic. Biol. Med. 2015, in press. Law CH, Kong PW, Szeto SSW, Li G, Quan Q, Zhang Z, Chu IK. ""A Versatile Reversed Phase-Strong Cation Exchange-Reversed Phase (RP-SCX-RP) Multidimensional Liquid Chromatography Platform for Qualitative and Quantitative Shotgun Proteomics."" Analyst. 2015, 140, 1237-1252. Zhao Y, Szeto SSW, Kong PW, Law CH, Li G, Quan Q, Zhang Z, Chu IK. ""Online Two-Dimensional Porous Graphitic Carbon/Reversed Phase Liquid Chromatography Platform Applied to Shotgun Proteomics and Glycoproteomics.""Anal. Chem. 2014, 86 (24), 12172-12179. Lai CK, Mu X, Hao Q, Hopkinson AC, Chu IK. ""Formation, isomerization, and dissociation of ε- and α-carbon-centered tyrosylglycylglycine radical cations."" Phys. Chem. Chem. Phys. 2014, 16 (44), 24235-24243. Mu X, Song T, Xu M, Lai CK, Siu CK, Laskin J, Chu IK. ""Discovery and Mechanistic Studies of Facile N‑Terminal Cα−C Bond Cleavages in the Dissociation of Tyrosine-Containing Peptide Radical Cations."" J. Phys. Chem. B. 2014, 118, 4273-4281. Quan Q, Song T, Hao Q, Siu CK, Chu IK. ""Mechanistic investigation of phosphate-ester bond cleavages of glycylphosphoserinyltryptophan radical cations under low-energy collision-induced dissociations."" J. Am. Soc. Mass Spectrom. 2013, 24 (4), 554-562. (Invited article) Song T, Chu IK, Siu CK, Laskin J. ""Mechanistic Examination of Cβ–Cγ Bond Cleavages of Tryptophan Residues During Dissociations of Molecular Peptide Radical Cations."" J. Phys. Chem. A 2013, 117 (6), 1059-1068. (Invited article) Lee CL, Lam MPY, Lam K, Leung CON, Pang R, Chu IK, Wan THL, Chai J, Yeung W, Chiu P. ""The identification of CD147 (Basigin) as a mediator of trophoblast functions."" Human Reproduction. 2013, 28 (11), 2920-2929. Wong MKH, Sze KH, Chen T, Cho CK, Law HCH, Chu IK, Wong AOL. ""Goldfish spexin: solution structure and novel function as a satiety factor in feeding control."" Am. J. Physiol. Endocrinol. Metab. 2013, 305 (3), E348-E366. Lai CK, Ng DCM, Pang HF, Cheung ASC, Chu IK. ""Laser-Induced Dissociation of Singly Protonated Peptides at 193 and 266 nm within a Hybrid Linear Ion Trap Mass Spectrometer."" Rapid Commun. Mass Spectrom. 2013, 27 (10), 1119-1127. Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J. ""Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis."" Plant J. 2013, 76 (6), 943-956. Wong BST, Lam KKW, Lee CL, Wong VHH, Lam MPY, Chu IK, Yeung WSB, Chiu PCN. ""Adrenomedullin Enhances Invasion of Human Extravillous Cytotrophoblast-Derived Cell Lines by Regulation of Urokinase Plasminogen Activator Expression and S-Nitrosylation."" Biology of Reproduction. 2013, 88 (2), 34. Kong RPW, Quan Q, Hao Q, Lai CK, Siu CK, Chu IK. ""Formation and Dissociation of Phosphorylated Peptide Radical Cations."" J. Am. Soc. Mass Spectrom. 2012, 23 (12), 2094-2101. Laskin J, Kong RPW, Song T, Chu IK. ""Effect of the basic residue on the energetics and dynamics of dissociation of phosphopeptides."" Int. J. Mass. Spectrom. 2012, 15 (330-332), 295-301. (Invited article) Hao Q, Song T, Ng DCM, Quan Q, Siu CK, Chu IK. ""Arginine-Facilitated Isomerization: Radical-Induced Dissociation of Aliphatic Radical Cationic Glycylarginyl(Iso)leucine Tripeptides."" J. Phys. Chem. B 2012, 116 (26), 7627-7634. Zhao JF, Siu KWM, Hopkinson AC, Song T, Xu MJ, Quan Q, Chu IK. ""Intramolecular hydrogen atom migration along the backbone of cationic and neutral radical tripeptides andsubsequent radical-induced dissociations."" Phys. Chem. Chem. Phys. 2012, 14 (24), 8723-8731. Zhao Y, Kong RPW, Li GH, Lam MPY, Law CH, Lee S, Lam HC, Chu IK. ""Fully automatable two-dimensional HILIC–RP liquid chromatography with online tandem mass spectrometry for shotgun proteomics."" J. Sep. Sci. 2012, 35, 1-9. (Invited article) Laskin J, Yang ZB, Lam C, Chu IK. ""Energy and entropy effects in dissociation of peptide radical anions."" Int. J. Mass. Spectrom. 2012, 316-318, 251– 258. Song T, Hao Q, Law CH, Siu CK, Chu IK. ""Novel Cβ –Cγ Bond Cleavages of Tryptophan-Containing Peptide Radical Cations."" J. Am. Soc. Mass Spectrom. 2012, 23 (2), 264-273. Zhang ZJ, Cheang LC, Wang MW, Li GH, Chu IK, Lin ZX, Lee SM. ""Ethanolic Extract of Fructus Alpinia oxyphylla Protects Against 6-Hydroxydopamine-Induced Damage of PC12 Cells In Vitro and Dopaminergic Neurons in Zebrafish."" Cell Mol Neurobiol. 2012, 32, 27–40.