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个人简介

M.S., Chemistry, University of Maine, 1983 Ph.D., Chemistry, University of Virginia, 1987 B.A., Zoology, University of Maine, 1980 Awards & Professional Activities Associate Editor, Analytical Chemistry Board of Reviewing Editors Science Biemann Award 2004 American Society for Mass Spectrometry Research Award 1996 Pehr Edman Award 1998

研究领域

Genomic and EST sequencing projects are providing a sequence infrastructure that is changing how protein biochemistry is practiced. These data along with mass spectrometry provide the cornerstone technologies fueling the proteomics revolution. Our group is focused on developing tools and strategies in proteomics to answer basic biological questions. By coupling the additional amino acid sequence information that can be obtained using peptide tandem mass spectrometry to "back-end" database searching and "front-end" separative techniques, we are able to directly analyze extremely complex protein mixtures. The applications for these technologies vary from analyzing purified protein complexes to studying host-pathogen interactions in diseases such as malaria or anthrax. We are further extending these applications to find post-translational modifications to the proteins within these same complex mixtures.

近期论文

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Markus Englert, Kelly Sheppard, Aaron Aslanian, John Yates 3rd, Dieter S�ll, Archaeal 3�-phosphate tRNA splicing ligase characterization identifies the missing component in tRNA maturation, PNAS (submitted) Sidonie Wicky John, Hendri Tjandra, David Schieltz, John Yates III, and Douglas R. Kellogg, The Zds proteins control entry into mitosis and target protein phosphatase 2A to the Cdc25 phosphatase (accepted for publication) Daniel B. McClatchy, Lujian Liao,Sung Kyu Park, Tao Xu, Bingwen Lu and John R. Yates III, Differential proteomic analysis of mammalian tissues using SILAM. PloSOne (in press) Samuel L. Hutson, Ernest Mui, Karen Kinsley, William H. Witola, Michael S. Behnke, Kamal El Bissati, Stephen P. Muench, Brittany Rohrman, Susan R. Liu, Robert Wollmann, Yuko Ogata, Ali Sarkeshik, John R. Yates III, and Rima McLeod, T. gondii RP Promoters & Knockdown Reveal Molecular Pathways Associated with Proliferation and Cell-cycle Arrest, PloSOne (in press) Paulo C Carvalho, Juliana S G Fischer, Jonas Perales, John R Yates III, Valmir C Barbosa, Elias Bareinboim, Analyzing marginal cases in differential shotgun proteomics, Bioinformatics (submitted) Alph�e Michelot, Michael Costanzo, Ali Sarkeshik, Charles Boone, John R. Yates III and David G. Drubin, Reconstitution and Protein Composition Analysis of Endocytic Actin Patches. Current Biology. (in press). Juliana de Saldanha da Gama Fischer 1,2, Paulo Costa Carvalho 1,3, Clovis Orlando da Fonseca4, Lujian Liao5, Maria da Gloria da Costa Carvalho6, Wim M Degrave2, John R Yates III4, and Gilberto B. Domont 7 A chemo-resistant protein expression pattern of glioblastoma cells (A172) to perillyl alcohol, Journal of Proteome Research (in press) Kristen Witte, Amber L. Schuh, Ali Sarkeshik, Jan Hegermann, Jonathan R. Mayers, Katrin Schwarze, John R. Yates III, Stefan Eimer, and Anjon Audhya, Mechanisms by which TFG functions in protein secretion and oncogenesis, Nature Cell Biology (submitted) Roberts-Galbraith RH, Ohi MD, Ballif BA, Chen JS, McLeod I, McDonald WH, Gygi SP, Yates JR 3rd, Gould KL., Dephosphorylation of F-BAR protein Cdc15 modulates its conformation and stimulates its scaffolding activity at the cell division site., Mol Cell. 2010, 39, 86-99 Welburn JP, Vleugel M, Liu D, Yates JR 3rd, Lampson MA, Fukagawa T, Cheeseman IM., Aurora B phosphorylates spatially distinct targets to differentially regulate the kinetochore-microtubule interface., Mol Cell. 2010, 38, 383-92. Nakorchevsky A, Hewel JA, Kurian SM, Mondala TS, Campbell D, Head SR, Marsh CL, Yates JR 3rd, Salomon DR., Molecular mechanisms of chronic kidney transplant rejection via large-scale proteogenomic analysis of tissue biopsies. J Am Soc Nephrol. 2010, 21, 362-73. Zhenming Xu, Zsolt Fulop1�, Guikai Wu, Egest J. Pone, Jinsong Zhang, Thach Mai, Lisa M. Thomas, Ahmed Al-Qahtani, Clayton A. White, Seok-Rae Park�, Petra Steinacker, Zenggang Li, John Yates, III, Bruce Herron, Markus Otto, Hong Zan, Haian Fu & Paolo Casali, 14-3-3 adaptor proteins recruit AID to 5''-AGCT-3'-rich switch regions for class switch recombination, Nature Structural and Molecular Biology (in press) Huang, H.*, Jeon, M.*, Liao, L.*, Yang, C.*, Elly, C., Yates, J.R., and Liu YC. Unconventional K33-linked polyubiquitination of TCR? regulates T cell activation by modulating endocytosis-independent TCR phosphorylation. Immunity. (accepted) Pham, Emiley; Crews, Leslie; Ubhi, Kiren; Hansen, Lawrence; Adame, Anthony; Cartier, Anna; Salmon, David; Galasko, Douglas; Michael, Sarah; Savas, Jeffrey; Yates, John; Glabe, Charles; Masliah, Eliezer, Progressive accumulation of amyloid-� oligomers in Alzheimer�s disease and APP transgenic mice is accompanied by selective alterations in synaptic scaffold proteins, FEBS (in press) Kops GJ, van der Voet M, Manak MS, van Osch MH, Naini SM, Brear A, McLeod IX, Hentschel DM, Yates JR 3rd, van den Heuvel S, Shah JV., APC16 is a conserved subunit of the anaphase-promoting complex/cyclosome. J Cell Sci. 2010, 123, 1623-33. Bryan R. Fonslow, Seong A. Kang, Daniel R. Gestaut, Beth Graczyk, Trisha N. Davis, David M. Sabatini, and John R. Yates III, Native capillary isoelectric focusing for the separation of protein complex isoforms and subcomplexes, Analytical Chemistry 2010, 82, 6643-51. Louis Alexander Saddic; Lisandra E. West; Aaron Aslanian; John Yates; Seth M. Rubin; Or Gozani, Julien Sage, Methylation of the retinoblastoma tumor suppressor by SMYD2, Journal of Biological Chemistry, M110.137612 First Published on September 24, 2010, Junling Wang, Xuemei Han, Sougata Saha, Tao Xu, Reena Rai, Fangliang Zhang, Yuri. I. Wol3, Alexey Wolfson, John R. Yates, III, and Anna Kashina, Arginyltransferase is an atp-independent self-regulating enzyme that forms distinct functional complexes in vivo, Chemistry and Biology (in press) Lemmuel L. Tayo��, Bingwen Lu�, Lourdes J. Cruz�, John R. Yates III, Proteomic Analysis Provides Insights on Venom Processing in Conus textile, Journal of Proteome Research 2010, 9, 2292-301 Ophelia Papoulas, Kate Monzo, Greg Cantin, Cristian Ruse, John Yates, III, and John Sisson.dFMRP and Caprin, Translational Regulators of Synaptic Plasticity, Control the Cell Cycle Machinery at the Drosophila Mid-blastula Transition. PLos Biology (submitted)

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