Santos, Patricia C. Dos - Wake Forest University

个人简介

B.S., 1999, Universidade Federal do Rio Grande do Sul (Porto Alegre, Brazil) Ph.D., 2004, Virginia Tech Postdoctoral Associate, 2005-2008, Virginia Tech (Dennis Dean)

研究领域

Iron and sulfur atoms can form chemical structures of biological importance named [Fe-S] clusters. The importance of Fe-S clusters is intimately associated with the essential role of Fe-S proteins in a wide range of life sustaining processes including respiration, carbon metabolism, photosynthesis and nitrogen fixation. These chemical structures are organized in fundamental units of [2Fe-2S] and [4Fe-4S] clusters, or multimers, or fragments of these units. Although [Fe-S] clusters can be formed in vitro from their toxic individual components, almost all forms of life have developed specialized machineries dedicated to the assembly of these metal-centers. Our research group is interested in understanding the biochemical formation of [Fe-S] clusters in Gram-positive bacteria.

近期论文

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AN ABREVIATED PATHWAY FOR THE BIOSYNTHESIS OF 2-THIOURIDINE IN BACILLUS SUBTILIS. Black KA, Dos Santos PC. J Bacteriol. 2015 Mar 30. pii: JB.02625-14. [Epub ahead of print] PMID: 25825430 Shared-intermediates in the biosynthesis of thio-cofactors: Mechanism and functions of cysteine desulfurases and sulfur acceptors. Black KA, Dos Santos PC. Biochim Biophys Acta. 2015 Jun;1853(6):1470-1480. doi: 10.1016/j.bbamcr.2014.10.018. Epub 2014 Oct 27. Review. PMID: 25447671 Fe-S cluster biogenesis in Gram-positive bacteria: SufU is a zinc-dependent sulfur transfer protein. Selbach BP, Chung AH, Scott AD, George SJ, Cramer SP, Dos Santos PC. Biochemistry. 2014 Jan 14;53(1):152-60. doi: 10.1021/bi4011978. Epub 2013 Dec 23. PMID: 24321018 Cross-functionalities of Bacillus deacetylases involved in bacillithiol biosynthesis and bacillithiol-S-conjugate detoxification pathways. Fang Z, Roberts AA, Weidman K, Sharma SV, Claiborne A, Hamilton CJ, Dos Santos PC. Biochem J. 2013 Sep 1;454(2):239-47. doi: 10.1042/BJ20130415. PMID: 23758290 Free Article Protected sulfur transfer reactions by the Escherichia coli Suf system. Selbach BP, Pradhan PK, Dos Santos PC. Biochemistry. 2013 Jun 11;52(23):4089-96. doi: 10.1021/bi4001479. Epub 2013 May 30. PMID: 23672190 Functional Analysis of Bacillus subtilis Genes Involved in the Biosynthesis of 4-Thiouridine in tRNA. Rajakovich LJ, Tomlinson J, Dos Santos PC. J Bacteriol. 2012 Sep;194(18):4933-40. doi: 10.1128/JB.00842-12. Epub 2012 Jul 6. PMID: 22773787 Free PMC Article Distribution of nitrogen fixation and nitrogenase-like sequences amongst microbial genomes. Dos Santos PC, Fang Z, Mason SW, Setubal JC, Dixon R. BMC Genomics. 2012 May 3;13:162. doi: 10.1186/1471-2164-13-162. PMID: 22554235 Free PMC Article Catalytic mechanism of Sep-tRNA:Cys-tRNA synthase: sulfur transfer is mediated by disulfide and persulfide. Liu Y, Dos Santos PC, Zhu X, Orlando R, Dean DR, Söll D, Yuan J. J Biol Chem. 2012 Feb 17;287(8):5426-33. doi: 10.1074/jbc.M111.313700. Epub 2011 Dec 13. PMID: 22167197 Free PMC Article Molecular biology and genetic engineering in nitrogen fixation. Dos Santos PC. Methods Mol Biol. 2011;766:81-92. doi: 10.1007/978-1-61779-194-9_6. PMID: 21833862 Genomic analysis of nitrogen fixation. O'Carroll IP, Dos Santos PC. Methods Mol Biol. 2011;766:49-65. doi: 10.1007/978-1-61779-194-9_4. PMID: 21833860 Transcriptional profiling of nitrogen fixation in Azotobacter vinelandii. Hamilton TL, Ludwig M, Dixon R, Boyd ES, Dos Santos PC, Setubal JC, Bryant DA, Dean DR, Peters JW. J Bacteriol. 2011 Sep;193(17):4477-86. doi: 10.1128/JB.05099-11. Epub 2011 Jul 1. PMID: 21724999 Free PMC Article Co-ordination and fine-tuning of nitrogen fixation in Azotobacter vinelandii. Dos Santos PC, Dean DR. Mol Microbiol. 2011 Mar;79(5):1132-5. doi: 10.1111/j.1365-2958.2011.07541.x. Epub 2011 Jan 25. PMID: 21338415 Bioinorganic chemistry: electrons in Fe-S protein assembly. Dos Santos PC, Dean DR. Nat Chem Biol. 2010 Oct;6(10):700-1. doi: 10.1038/nchembio.438. No abstract available. PMID: 20852605 Kinetic analysis of the bisubstrate cysteine desulfurase SufS from Bacillus subtilis. Selbach B, Earles E, Dos Santos PC. Biochemistry. 2010 Oct 12;49(40):8794-802. doi: 10.1021/bi101358k. Epub 2010 Sep 16. PMID: 20822158 Characterization of the N-acetyl-α-D-glucosaminyl l-malate synthase and deacetylase functions for bacillithiol biosynthesis in Bacillus anthracis . Parsonage D, Newton GL, Holder RC, Wallace BD, Paige C, Hamilton CJ, Dos Santos PC, Redinbo MR, Reid SD, Claiborne A. Biochemistry. 2010 Sep 28;49(38):8398-414. doi: 10.1021/bi100698n. PMID: 20799687 Free PMC Article Mobilization of sulfane sulfur from cysteine desulfurases to the Azotobacter vinelandii sulfurtransferase RhdA. Cartini F, Remelli W, Dos Santos PC, Papenbrock J, Pagani S, Forlani F. Amino Acids. 2011 Jun;41(1):141-50. doi: 10.1007/s00726-010-0529-z. Epub 2010 Mar 7. PMID: 20213443 A substrate channel in the nitrogenase MoFe protein. Barney BM, Yurth MG, Dos Santos PC, Dean DR, Seefeldt LC. J Biol Inorg Chem. 2009 Sep;14(7):1015-22. doi: 10.1007/s00775-009-0544-2. Epub 2009 May 21. PMID: 19458968 Free PMC Article A newly discovered role for iron-sulfur clusters. Dos Santos PC, Dean DR. Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11589-90. doi: 10.1073/pnas.0805713105. Epub 2008 Aug 12. No abstract available. PMID: 18697949 Free PMC Article In vivo iron-sulfur cluster formation. Raulfs EC, O'Carroll IP, Dos Santos PC, Unciuleac MC, Dean DR. Proc Natl Acad Sci U S A. 2008 Jun 24;105(25):8591-6. doi: 10.1073/pnas.0803173105. Epub 2008 Jun 17. PMID: 18562278 Free PMC Article A proposed role for the Azotobacter vinelandii NfuA protein as an intermediate iron-sulfur cluster carrier. Bandyopadhyay S, Naik SG, O'Carroll IP, Huynh BH, Dean DR, Johnson MK, Dos Santos PC. J Biol Chem. 2008 May 16;283(20):14092-9. doi: 10.1074/jbc.M709161200. Epub 2008 Mar 13. PMID: 18339629 Free PMC Article