Mao, Chengde - Purdue University - Department of Chemistry

个人简介

B.S., 1986, Peking University Ph.D., 1999, New York University (with Prof. Nadrian C. Seeman) Postdoctoral Fellow, 1999-2000, New York University (with Prof. Nadrian C. Seeman) Postdoctoral Fellow, 2001-2002, Harvard University (with Prof. George M. Whitesides)

研究领域

We are interested in programmed self-assembly of nucleic acids (DNA and RNA), or DNA nanotechnology. Nucleic acids are information-rich molecules. They have well-defined secondary structures (duplexes) and simple interaction rules (Watson-Crick base pairing). These chemical properties render nucleic acids to be excellent molecules for programmed self-assembly. Since 1982, a wide range of nanostructures have been constructed and find applications in biosensing, imaging, smart drug delivery, organizing chemical reactions, plasmonic devices etc. Our current research topics include: 1. DNA/RNA self-assembly. A key question that we would like to address is: how can we prepare complicated DNA nanostructures simply from minimal numbers of unique component DNA/RNA strands? In this regards, we are extensively taking advantage of structure/sequence symmetries. 2. Structural determination of biomacromolecules. The central idea is to organize biomacromolecules onto self-assembled DNA nanostructures to form highly symmetric biomacromolecule-DNA complexes, which can be readily characterized by X-ray crystallography or cryo-EM imaging. 3. DNA-directed guest self-assembly. DNA nanostructures can serve as a platform to organize/integrate multi-functionalities (such as molecular recognition, catalysis, and light harvesting). 3. DNA-based molecular lithography. We are developing methods to transfer DNA nanostructures into nanostructures of functional materials, e.g. silicone. 4. DNA nanomachines. Conformational changes of DNA can be programmed to perform particular functions, e.g. transporting materials along prescribed paths. 5. DNA-based nanomedicines. We are exploring using DNA nanostructures as platforms for sensitive disease diagnosis, bioimaging, and smart drug (small nucleic acid drugs in particular) delivery, and therapeutic devices. 6. Interrogation of basic biological processes/interactions related to spatial-temporal controls. Among many biological problems of our interest, multivalency is particularly important. It is a common strategy in biological events, ranging from immune responses to cell interactions. DNA nanostructures provide a universal tool to organize ligands for studying multivalent interactions.

近期论文

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Cuizheng Zhang, Jiemin Zhao, Brandon Lu, Nadrian C. Seeman, Ruojie Sha, Nicholas Noinaj, and Chengde Mao. Engineering DNA Crystals toward Studying DNA–Guest Molecule Interactions. J. Am. Chem. Soc. (accepted. Online released on Feb. 15, 2023). Victoria Paluzzi, Cuizheng Zhang, Chengde Mao. Near Quantitative Preparation of Short, Single-Stranded DNA Circles. Angew. Chem. Intl. Ed. (accepted. Online released Jan. 18, 2023). Simon Vecchioni, Brandon Lu, Jordan Janowski, Karol Woloszyn, Nataša Jonoska, Nadrian C Seeman, Chengde Mao, Yoel P Ohayon, Ruojie Sha. The Rule of Thirds: Controlling Junction Chirality and Polarity in 3D DNA Tiles. Small (accepted. Online released on Dec. 30, 2022). Jie Zeng, Liang Fang, Tingting Jiang, Mingchun Liu, Wei Li, Chengde Mao, Shifeng Lou, Hua Zuo. Structural‐Based Stability Enhancement of Antisense DNA Oligonucleotides. Macromol. Biosci. (accepted. Online released Dec. 21, 2022). Yue Zhao, Arun Richard Chandrasekaran, David A Rusling, Karol Woloszyn, Yudong Hao, Carina Hernandez, Simon Vecchioni, Yoel P Ohayon, Chengde Mao, Nadrian C Seeman & Ruojie Sha. The Formation and Displacement of Ordered DNA Triplexes in Self-Assembled Three-Dimensional DNA Crystals. J. Am. Chem. Soc. 145, 3599-3605 (2023). Brandon Lu, Karol Woloszyn, Yoel P Ohayon, Bena Yang, Cuizheng Zhang, Chengde Mao, Nadrian C Seeman, Simon Vecchioni & Ruojie Sha. Programmable 3D Hexagonal Geometry of DNA Tensegrity Triangles. Angew. Chem. Intl. Ed. 62, e202213451 (2023). Brandon Lu, Simon Vecchioni, Yoel P Ohayon, Karol Woloszyn, Tiffany Markus, Chengde Mao, Nadrian C Seeman, James W Canary & Ruojie Sha. Highly Symmetric, Self‐Assembling 3D DNA Crystals with Cubic and Trigonal Lattices. Small 19, 2205830 (2023). Dake Mao, Victoria E Paluzzi, Cuizheng Zhang & Chengde Mao. DNA conformational equilibrium enables continuous changing of curvatures. Nanoscale 15, 470-475 (2023). Zhe Zhang, Huaiqing Chen, Liang Fang, Huawei He, Chengde Mao & Hua Zuo. Solution-Phase Synthesis of DNA Amphiphiles for DNA Micellar Assembly. Bioconjugate Chem. 34, 85-91 (2023). Nanpu Cao, Wanhao Cai, Lu Qian, Zhou Nie, Chengde Mao & Shuxun Cui. Emulating Titin by a Multidomain DNA Structure. ACS Macro Lett. 12, 59-64 (2023). Xiaoli Hu, Linlin Tang, Mengxi Zheng, Jian Liu, Zhe Zhang, Zhe Li, Quan Yang, Shoubo Xiang, Liang Fang, Qiao Ren, Xuemei Liu, Cheng Zhi Huang, Chengde Mao & Hua Zuo. Structure-Guided Designing Pre-Organization in Bivalent Aptamers. J. Am. Chem. Soc. 144, 4507-4514 (2022). Cuizheng Zhang, Mengxi Zheng1, Yoel P. Ohayon, Simon Vecchioni, Ruojie Sha, Nadrian C. Seeman, Natasha Jonoska & Chengde Mao. Programming DNA Self-Assembly by Geometry. J. Am. Chem. Soc. 144, 8741–8745 (2022). Mengxi Zheng, Zhe Li, Cuizheng Zhang, Nadrian C. Seeman & Chengde Mao. Powering a ∼ 50-μm Motion by a Molecular Event in DNA Crystals. Adv. Mater. 34, 2200441 (2022). Karol Woloszyn, Simon Vecchioni, Yoel P Ohayon, Brandon Lu, Yinglun Ma, Qiuyan Huang, Eric Zhu, Daniel Chernovolenko, Tiffany Markus, Nataša Jonoska, Chengde Mao, Nadrian C Seeman, Ruojie Sha. Augmented DNA Nano-Architectures: A Structural Library of 3D Self-Assembling Tensegrity Triangle Variants. Adv. Mater. (Accepted. online released September 13, 2022). DOI: 10.1002/adma.202206876 Dake Mao, Qian Li, Qian Li Pengfei Wang & Chengde Mao. A Conformational Study of the 10-23 DNAzyme via Programmed DNA Self-Assembly. Chem. Commun. 58, 6188-6191 (2022). Victoria E. Paluzzi, Cuizheng Zhang & Chengde Mao. Assembly of Two‐Dimensional DNA Arrays Could Influence the Formation of Their Component Tiles. ChemBioChem 23, e202200306 (2022). Kaixuan Zhou, Zhichao Mei, Yunxiang Lei, Zhen Guan, Chengde Mao & Yulin Li. Boosted Productivity in Single‐Tile‐Based DNA Polyhedra Assembly by Simple Cation Replacement. ChemBioChem 23, e2022001 (2022). Cuizheng Zhang, Victoria E. Paluzzi & Chengde Mao. Tomography of DNA tiles influences the kinetics of surface-mediated DNA self-assembly. Biophysical J. 121, 1–6 (2022). Ruixin Li, Mengxi Zheng, Anirudh S Madhvacharyula, Yancheng Du, Chengde Mao & Jong Hyun Choi. Mechanical deformation behaviors and structural properties of ligated DNA crystals. Biophysical J. 121, 4078–4090 (2022) Mengxi Zheng, Zhe Li, Longfei Liu, Mo Li, Victoria E. Paluzzi, Jong Hyun Choi & Chengde Mao. Kinetic DNA Self-Assembly: Simultaneously Co-folding Complementary DNA Strands into Identical Nanostructures. J. Am. Chem. Soc. 143, 20363-20367 (2021). Zhe Li, Mengxi Zheng, Longfei Liu, Nadrian C. Seeman & Chengde Mao. 5′-Phosphorylation Strengthens Sticky-End Cohesions. J. Am. Chem. Soc. 143, 14987–14991 (2021). Rong Sheng LiRong Sheng Li, Jiahui Liu, Hu Shi, Ping Ping Hu, Yao Wang, Peng Fei Gao, Jian Wang, Moye Jia, Hongwei Li, Yuan Fang Li, Chengde Mao, Na Li & Cheng Zhi Huang. Transformable Helical Self-Assembly for Cancerous Golgi Apparatus Disruption. Nano Lett. 21, 8455-8564 (2021). Brandon Lu, Simon Vecchioni, Yoel P. Ohayon, Ruojie Sha, Karol Woloszyn, Bena Yang, Chengde Mao & Nadrian C. Seeman. 3D Hexagonal Arrangement of DNA Tensegrity Triangles. ACS Nano 15, 16788-16793 (2021). Yancheng Du, Jing Pan, Hengming Qiu, Chengde Mao & Jong Hyun Choi. Mechanistic Understanding of Surface Migration Dynamics with DNA Walkers. J. Phys. Chem. B 125, 507−517 (2021). Mengxi Zheng, Qian Li, Qian Li, Victoria E. Paluzzi, Jong Hyun Choi & Chengde Mao. Engineering the Nanoscaled Morphologies of Linear DNA Homopolymers. Macromol. Rapid Commun. 42, 2100217 (2021). Jiezhong Shi, Chenyou Zhu, Qian Li, Yujie Li, Liangxiao Chen, Bo Yang, Jiang-Fei Xu, Yuanchen Dong, Chengde Mao & Dongsheng Liu. Kinetically Interlocking Multiple-Units Polymerization of DNA Double Crossover and Its Application in Hydrogel Formation. Macromol. Rapid Commun. 42, 2100182 (2021). Kyoung-Ran Kim, Junghyun Kim, Chengde Mao & Dae-Ro Ahn. Kissing loop-mediated fabrication of RNA nanoparticles and their potential as cellular and in vivo siRNA delivery platforms. Biomater. Sci. 9, 8148-8152 (2021). Qiang Dong, Kaixuan Zhou, Ning Deng, Xiaojing Wu, Chengde Mao & Yulin Li. Regulating the Kinetics of DNA Attachment: Construction of Defined Clusters with High DNA Density and Strong Plasmonic Coupling. ChemNanoMat 7, 811 –814 (2021). Qian Li, Jiemin Zhao, Longfei Liu, Sagun Jonchhe, Felix J. Rizzuto, Shankar Mandal, Huawei He, Sansen Wei, Hanadi F. Sleiman, Hanbin Mao & Chengde Mao. A Poly(thymine) – Melamine Duplex for the Assembly of DNA Nanomaterials. Nat. Materials. 19, 1012-1018 (2020). Di Liu, Cody W. Geary, Gang Chen, Yaming Shao, Mo Li, Chengde Mao, Ebbe S. Andersen, Joseph A. Piccirilli, Paul W. K. Rothemund & Yossi Weizmann. Branched Kissing Loops for the Construction of Diverse RNA Homooligomeric Nanostructures. Nat. Chemistry 12, 249-259 (2020). Qian Li, Longfei Liu, Yuyan Yu, Weili Li, Xinfeng Zhao & Chengde Mao. ATP-Triggered, Allosteric Self-Assembly of DNA Nanostructures. J. Am. Chem. Soc. 142, 665-668 (2020). Kai He, Longfei Liu, Mengxi Zheng & Chengde Mao. Assembly of a DNA Origami Chinese Knot by Only 15% of the Staple Strands. ChemBioChem 15, 2132-2136 (2020). Peijun Shi, Zhe Zhang, Mingchun Liu, Teng Sun, Xioyan He, Chengde Mao, Liang Fang & Hua Zuo. Increasing the Solubility of a Hydrophobic Molecule with Thymine-like Face by DNA via Supramolecular Interaction. Chem. Res. Chin. Univ. 36, 281-284 (2019). Longfei Liu, Zhe Li, Yulin Li & Chengde Mao. Rational Design and Self-Assembly of Two-Dimensional, Dodecagonal DNA Quasicrystals. J. Am. Chem. Soc. 141, 4248-4251 (2019). Zhe Li, Longfei Liu, Mengxi Zheng, Jiemin Zhao, Nadrian C. Seeman & Chengde Mao. Making Engineered 3D DNA Crystals Robust. J. Am. Chem. Soc. 141, 15850-15855 (2019). Kai Huang, Donglei Yang, Shenyu Tan, Silian Chen, Ye Xiang, Yongli Mi, Chengde Mao & Bryan Wei. Self‐Assembly of Wireframe DNA Nanostructures from Junction Motifs. Angew. Chem. Intl. Ed. 58, 12123-12127 (2019). Wenhao Fu, Linlin Tang, Gaohui Wei, Liang Fang, Renjie Zhan, Xuemei Liu, Hua Zuo, Cheng Zhi Huang & Chengde Mao. Rational Design of pH-Responsive DNA Motifs with General Sequence Compatibility. Angew. Chem. Intl. Ed. 58, 16405-16410 (2019). Yoel P. Ohayon, Carina Hernandez, Arun Richard Chandrasekaran, Xinyu Wang, Hatem O. Abdallah, Michael Alexander Jong, Michael G. Mohsen, Ruojie Sha, Jens J. Birktoft, Philip S. Lukeman, Paul M. Chaikin, Stephen L. Ginell, Chengde Mao &Nadrian C. Seeman. Designing Higher Resolution Self-Assembled 3D DNA Crystals via Strand Terminus Modifications. ACS Nano. 13, 7957-7965 (2019). Longfei Liu, Mengxi Zheng, Zhe Li, Qian Li & Chengde Mao. Patterning Nanoparticles with DNA Molds. ACS Appl. Mater. Interfaces 11, 13853-13858 (2019). Jie Zeng, Wenhao Fu, Zhenping Qi, Qiushuang Zhu, Huawei He, Chengzhi Huang, Hua Zuo & Chengde Mao. Self-Assembly of Microparticles by Supramolecular Homopolymerization of One Component DNA Molecule. Small 15, 1805552 (2019). Hua Zuo & Chengde Mao. A minimalist's Approach for DNA Nanoconstructions. Adv. Drug Deliv. Rev. 147, 22-28 (2019). Xing Wang, Arun Richard Chandrasekaran, Zhiyong Shen, Yoel P. Ohayon, Tong Wang, Megan E. Kizer, Ruojie Sha, Chengde Mao, Hao Yan, Xiaoping Zhang, Shiping Liao∥, Baoquan Ding, Banani Chakraborty, Natasha Jonoska, Dong Niu, Hongzhou Gu, Jie Chao, Xiang Gao, Yuhang Li, Tanashaya Ciengshin & Nadrian C. Seeman. Paranemic Crossover DNA: There and Back Again. Chem. Rev. 17, 6273-6289 (2019). Kyoung-Ran Kim, Seong Jae Kang, A-Young Lee, Dohyeon Hwang, Miri Park, Haedong Park, Sanghee Kim, Kahyun Hur, Hak Suk Chung, Chengde Mao & Dae-Ro Ahn. Highly Tumor-Specific DNA Nanostructures Discovered by in vivo Screening of a Nucleic Acid Cage Library And Their Applications in Tumor-Targeted Drug Delivery. Biomaterials 195, 1-12 (2019). Qian Liu, Dong Wang, Zhi Xu, Chunji Huang, Chun Zhang, Binfeng He, Chengde Mao, Guansong Wang & Hang Qian. Targeted Delivery of Rab26 siRNA with Precisely Tailored DNA Prism for Lung Cancer Therapy. Chembiochem 20, 1139-1144 (2019). Linlin Tang, Chengzhi Huang, Hua Zuo & Chengde Mao. DNA Nanotechnology in Molecular Design of Thrombin Aptamers. Chin. Sci. Bull. 64, 1018-1026 (2019). Linlin Tang, Jialian Tan, Yi Xiang, Zhubo Li, Chengde Mao & Hua Zuo. DNA-NH2 Acylation Reaction: Molecular Weight or Conformation Dominates?. Chin. Sci. Bull. 64, 1076-1083 (2019). Mo Li, Mengxi Zheng, Siyu Wu, Cheng Tian, Di Liu, Yossi Weizmann, Wen Jiang, Guansong Wang & Chengde Mao. In Vivo Production of RNA Nanostructures via Programmed Golding of Single-Stranded RNAs. Nat. Commun. 9, 2196 (2018). Yongfei Li, Lei Song, Bang Wang, Jianbo He, Yulin Li, Zhaoxiang Deng & Chengde Mao. Universal pH-Responsive and Metal-Ion-Free Self-Assembly of DNA Nanostructures. Angew. Chem. Intl. Ed. 57, 6892-6895 (2018). Jiemin Zhao, Yue Zhao, Zhe Li, Yong Wang, Ruojie Sha, Nadrian C. Seeman & Chengde Mao. Modulating Self‐Assembly of DNA Crystals with Rationally Designed Agents. Angew. Chem. Intl. Ed. 57, 16529-16532 (2018) Qian Liu, Dong Wang, M. Yuan, Binfeng He, Jin Li, Chengde Mao, Guansong Wang & Hang Qian. Capturing Intracellular Oncogenic MicroRNAs with Self-Assembled DNA Nanostructures for MicroRNA-Based Cancer Therapy. Chem. Sci. 9, 7562-7568 (2018). Qian Li, Cheng Tian, Xiang Li & Chengde Mao. Can Strand Displacement Take Place in DNA Triplexes?. Org. & Biomol. Chem. 16, 372-375 (2018). Dong Wang, Qian Liu, Di Wu, Binfeng He, Jin Li, Chengde Mao, Guansong Wang & Hang Qian. Isothermal Self-Assembly of Spermidine–DNA Nanostructure Complex as a Functional Platform for Cancer Therapy. ACS Appl. Mater. Interfaces 10, 15504-15516 (2018). Hua Zuo & Chengde Mao. Drugging the Undruggable Molecules by a DNA Aanorobot. Sci. China-Chem. 61, 763-764 (2018).