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个人简介

教育背景 1987 – 1991 西北农林科技大学(学士) 1998 – 2001 德累斯顿工业大学(硕士) 2004 – 2012 德累斯顿工业大学(博士) 工作经历 1991 – 1998 西北农林科技大学 2002 – 2004 基因桥公司(Gene Bridges GmbH) 2004 – 2014 德累斯顿工业大学生物技术中心 2014 – 微生物技术国家重点实验室,生命科学学院,山东大学,

研究领域

1. DNA同源重组机理研究,基因克隆技术开发。 2. 基因打靶技术和BAC转基因技术研究;转基因动物。 3. 细菌次生代谢产物基因簇的克隆,修饰和异源表达;药物开发。 4. 病毒基因组DNA文库构建和重组疫苗开发。

近期论文

查看导师新发文章 (温馨提示:请注意重名现象,建议点开原文通过作者单位确认)

1. Wenli Zhang,Jun Fu, Jing Liu, Hailong Wang, Maren Schiwon, Sebastian Janz, Lukas Schaffarczyk, Lukas von der Goltz, Eric Ehrke-Schulz, Johannes Dörner, Manish Solanki, Philip Boehme, Thorsten Bergmann, Andre Lieber, Chris Lauber, Andreas Dahl, Andreas Petzold, Youming Zhang, A. Francis Stewart and Anja Ehrhardt (2017) “An Engineered Virus Library as a Resource for the Spectrum-wide Exploration of Virus and Vector Diversity” Cell Reports. 19(8):1698-1709. 2. Hailong Wang, Zhen Li, Ruonan Jia, Yu Hou, Jia Yin, Xiaoying Bian, Aiying Li, Rolf Müller, A Francis Stewart,Jun Fu& Youming Zhang (2016) "A concerted pipeline for direct cloning of biosynthetic gene clusters and engineering for heterologous expression" Nature Protocols 11(7): 1175-90. 3. Oliver Baker, Ashish Gupta, Mandy Obst, Youming Zhang, Konstantinos Anastassiadis,Jun Fuand A. Francis Stewart (2016) “RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses” Scientific Reports 2016 May 24;6:25529. doi: 10.1038/srep25529. 4. Yin, J., Zhu, H., Xia, L., Ding, X., Hoffmann, T., Hoffmann, M., Bian, X., Müller, R.,Fu, J., Stewart, A.F., Zhang, Y. (2015) “A new recombineering system for Photorhabdus and Xenorhabdus” Nucleic Acids Research 2015 Mar 31;43(6):e36. doi: 10.1093/nar/gku1336 5. Wang, H., Bian, X., Xia, L., Ding, X., Müller, R., Zhang, Y.,Fu, J.and Stewart, A. F. (2014) " Improved seamless mutagenesis by recombineering using ccdB for counterselection " Nucleic Acids Research 2013 Dec 24. [Epub ahead of print] 6. Filipczyk, A., Gkatzis, K.,Fu, J., Lickert, H., Anastassiadis, K. and Schroeder, T. (2013) " Biallelic Expression of Nanog Protein in Mouse Embryonic Stem Cells" Cell Stem Cell 13:12-13 7. Rostovskaya, M.,Fu, J., Obst, M., Baer, I., Weidlich, S., Wang, H., Smith, A., Anastassiadis, K. and Stewart, A. F. (2012) “Transposon-Mediated BAC Transgenesis in Human ES Cells” Nucleic Acids Research, 2012 Jun 30. [Epub ahead of print] doi: 10.1093/nar/gks643 8.Fu, J., Bian, X., Hu, S., Wang, H., Huang, F., Seibert, P., Plaza, A., Xia, L., Müller, R., Stewart, A. F. and Zhang, Y. (2012) "Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting.” Nature Biotechnology 30: 440-446. 9. Bird, A. W., Erler, A.,Fu, J., Maresca, M., Heriche, J. K., Zhang, Y., Hyman, A. A. and Stewart, A. F. (2011) "High-efficiency counterselection recombineering for site-directed mutagenesis in bacterial artificial chromosomes." Nature Methods 9:103-109. 10. Nedelkova, M., Maresca, M.,Fu, J.,Rostovskaya, M., Thiede, C., Anastassiadis, K., Sarov, M. and Stewart, A. F. (2011). "Targeted isolation of genomic regions by recombineering." Nucleic Acids Research 39:e137. 11. Daniel, K., Lange, J., Hached, K.,Fu, J., Anastassiadis, K., Roig, I., Cooke, H. J., Stewart, A. F., Wassmann, K., Jasin, M., Keeney, S. and Toth, A. (2011). "Meiotic homologue alignment and its quality surveillance are controlled by mouse HORMAD1." Nature Cell Biology 13:599-610. 12. Skarnes, W. C., Rosen, B., West, A. P., Koutsourakis, M., Bushell, W., Iyer, V., Cox, R., Jackson, D., Severin, J., Biggs, P., Thomas, M., Mujica, A., Harrow, J.,Fu, J.,Nefedov, M., de Jong, P., Stewart, A. F. and Bradley, A. (2011). "A conditional knockout resource for genome-wide analysis of mouse gene function." Nature 474: 337-42. 13.Fu, J., Teucher, M., Anastassiadis, K., Skarnes, W. and Stewart, A. F. (2010). "A Recombineering pipeline to make conditional targeting constructs." Methods in Enzymology 477C: 125-144. 14.Fu, J., Wenzel, S. C., Perlova, O., Wang, J., Gross, F., Tang, Z., Yin, Y., Stewart, A. F., Müller, R. and Zhang, Y. (2008). "Efficient transfer of two large secondary metabolite pathway gene clusters into heterologous hosts by transposition." Nucleic Acids Research 36: e113.

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