研究领域

MusculaDystrophansignatransductio ;Transcriptiofactopurification

We contribute to two fields: Signal transduction, and more specifically the role of Ca2+ and its receptor protein, calmodulin, in cellular regulation We have shown that dystrophin, the protein product of the Duchenne muscular dystrophy gene locus, is a specific calmodulin-binding protein and discovered a signal transduction pathway, which links laminin-α-dystroglycan-β-dystroglycan-dystrophin-syntrophin-Grb2-Sos1-Rac1-PAK1-MKK4/7-JNK, causing c-jun phosphorylation and activation. This work is supported by National Institutes of Health grant 1R01AR051440. Transcription factor purification and characterization We first coupled DNA to HPLC silica and have made a significant contribution to the purification and characterization of transcription factors. Most recently we developed systematic oligonucleotide trapping, a method for purifying transcription factors to near homogeneity in a single step. Current experiments involve promoter trapping, a method which interfaces DNA-HPLC with proteomic techniques (MALDI-TOF and ESE-ion trap) to characterize the transcription complex of the c-jun promoter. This work is supported by National Institutes of Health grant 1R01GM43609.

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Linda I. Nagore; Harry W. Jarrett T3: Targeted proteomics of DNA-binding proteins Analytical Biochemistry. 2015;474:8-15. 0 Lishi Wang; Yan Jiao; Shuqiu Sun; Harry W. Jarrett; Dianjun Sun; Weikuan Gu Gene network of a phosphoglycerate mutase in muscle wasting in mice Cell Biology International. 2015. 0 Yinshan Jia; Oleg Larionov; Harry W. Jarrett Coupling of deoxyribonucleic acid to solid supports using 3' terminal ribose incorporation Journal of Chromatography A. 2014;1339:73-79. 0 L.I. Nagore; R.J. Nadeau; Q. Guo; Y.L.A. Jadhav; H.W. Jarrett; W.E. Haskins Purification and characterization of transcription factors Mass Spectrometry Reviews. 2013;32(5):386-398. 1 Christoph Hoffmann; Anika Zimmermann; Anke Hinney; Anna-Lena Volckmar; Harry W. Jarrett; Tobias Fromme; Martin Klingenspor A novel SP1/SP3 dependent intronic enhancer governing transcription of the UCP3 gene in brown adipocytes PLoS ONE. 2013;8(12). 1 Harry W. Jarrett Proteomic methodologies to study transcription factor function Methods in Molecular Biology. 2012;786:315-334. 1 YanWen Zhou; Yinshan Jia; Harry W. Jarrett Asymmetric polymerase chain reaction provides alternatives for preparation of (GT) 5-tailed duplex DNA promoter for promoter trapping Analytical Biochemistry. 2012;427(2):133-138. 0 Yan Wen Zhou; Jesus Munoz; Daifeng Jiang; Harry W. Jarrett Laminin-α 1 LG4-5 domain binding to dystroglycan mediates muscle cell survival, growth, and the AP-1 and NF-κb transcription factors but also has adverse effects American Journal of Physiology - Cell Physiology. 2012;302(6):C902-C914. 1 Daifeng Jiang; Srinivas Mummidi; Sunil K. Ahuja; Harry W. Jarrett CCR5 promoter haplotype transcription complex characterization Journal of Health Care for the Poor and Underserved. 2011;22(4 SUPPL.):73-90. 0 Daifeng Jiang; Yinshan Jia; Harry W. Jarrett Transcription factor proteomics: Identification by a novel gel mobility shift-three-dimensional electrophoresis method coupled with southwestern blot and high-performance liquid chromatography-electrospray-mass spectrometry analysis Journal of Chromatography A. 2011;1218(39):7003-7015. 3 Yinshan Jia; Daifeng Jiang; Harry W. Jarrett Repeated probing of Southwestern blots using alkaline phosphatase stripping Journal of Chromatography A. 2010;1217(45):7177-7181. 1 Jesus Munoz; Yanwen Zhou; Harry W. Jarrett LG4-5 domains of laminin-211 binds α-dystroglycan to allow myotube attachment and prevent anoikis Journal of Cellular Physiology. 2010;222(1):111-119. 16 Shoulei Jiang; Maria R. Galindo; Harry W. Jarrett Purification and identification of a transcription factor, USF-2, binding to E-box element in the promoter of human telomerase reverse transcriptase (hTERT) Proteomics. 2010;10(2):203-211. 7 Yan Jiao; Xiudong Jin; Jian Yan; Feng Jiao; Xinmin Li; Bruce A. Roe; Harry W. Jarrett; Weikuan Gu An insertion of intracisternal A-particle retrotransposon in a novel member of the phosphoglycerate mutase family in the lew allele of mutant mice Genes and Genetic Systems. 2009;84(5):327-334. 1 Yongmin Xiong; Yanwen Zhou; Harry W. Jarrett Dystrophin glycoprotein complex-associated Gβγ subunits activate phosphatidylinositol-3-kinase/akt signaling in skeletal muscle in a laminin-dependent manner Journal of Cellular Physiology. 2009;219(2):402-414. 29 Daifeng Jiang; Yinshan Jia; YanWen Zhou; Harry W. Jarrett Two-dimensional Southwestern blotting and characterization of transcription factors on-blot Journal of Proteome Research. 2009;8(7):3693-3701. 12 Daifeng Jiang; Harry W. Jarrett; William E. Haskins Methods for proteomic analysis of transcription factors Journal of Chromatography A. 2009;1216(41):6881-6889. 25 Markandeswar Panda; Daifeng Jiang; Harry W. Jarrett Trapping of transcription factors with symmetrical DNA using thiol-disulfide exchange chemistry Journal of Chromatography A. 2008;1202(1):75-82. 5 Daifeng Jiang; YanWen Zhou; Robert A. Moxley; Harry W. Jarrett Purification and identification of positive regulators binding to a novel element in the c-jun promoter Biochemistry. 2008;47(35):9318-9334. 13 Linda I. Nagore; Sucharita Mitra; Daifeng Jiang; Shoulei Jiang; Yan Wen Zhou; Magda Loranc; Harry W. Jarrett Cyanogen bromide-activated coupling: DNA catalytic chromatography purification of EcoRI endonuclease Nature Protocols. 2007;1(6):2909-2915.