个人简介

B.S. (Chemistry) 1953 University of Illinois, Urbana,IL Ph.D. (Biochemistry) 1957 Cornell University, Ithaca, NY

研究领域

Biochemistry

Biological chemical reactions are regulated by biological catalysts known as enzymes. There are also nonenzymatic chemical reactions in biological systems that are spontaneous and unregulated. One of these well studied nonenzymatic reactions, associated with the complications in diabetes, is that between elevated blood and tissue glucose concentrations and the amino group of proteins in a reaction known as glycation.These simple glycated proteins, which can form with any sugar, continue to react to form complex structures called advanced glycation endproducts (AGEs). AGEs have also been implicated with the pathology of a number of diseases besides diabetes including aging, arthritis, and Alzheimer’s.disease. Our lab has been involved in studies of AGEs formation with some of the sugars found in humans that are formed from glucose and absorbed from the diet. These include fructose, galactose, ribose, glyceraldhyde and glucosamine.. Glucosamine is a naturally occurring analog of glucose where an amine replaces the hydroxyl group on carbon-2 is of paticular interest to us. It is sold over the counter and is widely used as a dietary supplement to relieve the symptoms of osteoarthritis. Its effectiveness has been reported in many clinical studies, but none has identified the effect as being due to glucosamine alone or any of the many AGEs it can form. Our laboratory is developing capillary electrophoresis (CE) and high performance liquid chromatography methods to describe the in vitro formation of previously undescribed AGEs.

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Ganea, E., J.A. Dain, D.E. Rhoads (1992) Changes in the Absorption and Fluorescence Spectra of Non-enzymatically Glycosylated Proteins. Rev. roum. Biochim. 29, 173-178. Ganea, E., C. Amarandei, J.A. Dain (1994). Isolation of Galactose-1-Phosphatase from Rat Liver. Rev. roum. Biochim. 31, 23-27. Tutui, T., E. Ganea, J.A. Dain (1997) Rat Liver Versus Brain Galactose-1-Phosphatase. Rev. roum. Biochim. 34, 1-4. De Sa, P., J.L. Treubig, P.R. Brown & J.A. Dain (2001). The use of capillary electrophoresis to monitor Maillard Reaction Products (MRP) by glyceraldehyde and the epsilon amino group of lysine. Food Chem., 72, 379-384. De Sa, P.F.G.,C. Robb,E. Resende., P.McCarthy.,S.Yang,., P.R.Brown,.,J.A. Dain(2002). Capillary Electrophoretic Separation by Double-Strand Polyaniline Coated Capillaries of the Advanced Glycation Endproducts (AGE) formed from N-α-acetyl-L-Lysine with Reducing Sugars. J. Capillary Electrophoresis. 7, 61-65. Zhang, X., Y. Ma, H.Liu, P.F.G. Sa, P.R.Brown, J.A.Dain (2003).Capillary Electrophoresis Separation of Autocondensation Products of Glucosamiine Capillary Electrophoresis.8, 33-3 Zhang, X. Y. Ma, H. Liu, P.F.G. Sa, P.R.Brown, J.A. Dain (2004).Monitoring the formation of Maillard reaction products of glucosamine with fibrinogen and human serum albumin using capillary electrophoresis. Anal.Biochem. 325, 255-259. Dutta, U., J.A. Dain (2005).Capillary Electrophoretic Analysis of Advanced Glycation Endproducts formed from the reaction of reducing sugars with the amino group of Glucosamine .Anal.Biochem. 343, 237-243. Dutta, U., M.A. Cohenford, J.A .Dain (2005). Nonenzymatic Glycation of DNA nucleosides with reducing Sugars Anal.Biochem. 345, 171-180. Dutta, U., M.A. Cohenford, J.A. Dain (2006).Monitoring the effect of glyceraldehyde glycation on the secondary structure human serum albumin and immunoglobulin G: An analysis based on circular dichromism, thermal melting profiles and UV-fluorescence spectroscopy. Anal.Clin, Acta. 558 Ganea, E., J.A. Dain, D.E. Rhoads (1992) Changes in the Absorption and Fluorescence Spectra of Non-enzymatically Glycosylated Proteins. Rev. roum. Biochim. 29, 173-178. Ganea, E., C. Amarandei, J.A. Dain (1994). Isolation of Galactose-1-Phosphatase from Rat Liver. Rev. roum. Biochim. 31, 23-27. Tutui, T., E. Ganea, J.A. Dain (1997) Rat Liver Versus Brain Galactose-1-Phosphatase. Rev. roum. Biochim. 34, 1-4. De Sa, P., J.L. Treubig, P.R. Brown & J.A. Dain (2001). The use of capillary electrophoresis to monitor Maillard Reaction Products (MRP) by glyceraldehyde and the epsilon amino group of lysine. Food Chem., 72, 379-384. De Sa, P.F.G.,C. Robb,E. Resende., P.McCarthy.,S.Yang,., P.R.Brown,.,J.A. Dain(2002). Capillary Electrophoretic Separation by Double-Strand Polyaniline Coated Capillaries of the Advanced Glycation Endproducts (AGE) formed from N-α-acetyl-L-Lysine with Reducing Sugars. J. Capillary Electrophoresis. 7, 61-65. Zhang, X., Y. Ma, H.Liu, P.F.G. Sa, P.R.Brown, J.A.Dain (2003).Capillary Electrophoresis Separation of Autocondensation Products of Glucosamiine Capillary Electrophoresis.8, 33-3 Zhang, X. Y. Ma, H. Liu, P.F.G. Sa, P.R.Brown, J.A. Dain (2004).Monitoring the formation of Maillard reaction products of glucosamine with fibrinogen and human serum albumin using capillary electrophoresis. Anal.Biochem. 325, 255-259. Dutta, U., J.A. Dain (2005).Capillary Electrophoretic Analysis of Advanced Glycation Endproducts formed from the reaction of reducing sugars with the amino group of Glucosamine .Anal.Biochem. 343, 237-243. Dutta, U., M.A. Cohenford, J.A .Dain (2005). Nonenzymatic Glycation of DNA nucleosides with reducing Sugars Anal.Biochem. 345, 171-180. Dutta, U., M.A. Cohenford, J.A. Dain (2006).Monitoring the effect of glyceraldehyde glycation on the secondary structure human serum albumin and immunoglobulin G: An analysis based on circular dichromism, thermal melting profiles and UV-fluorescence spectroscopy. Anal.Clin, Acta. 558