Arkov, Alexey - Murray State University

个人简介

Ph.D., University of Texas M.D. Anderson Cancer Center, Houston TX (laboratory of Dr. Emanuel J. Murgola) Postdoctoral research, Skirball Institute of Biomolecular Medicine at New York University School of Medicine (laboratory of Dr. Ruth Lehmann)

研究领域

Our laboratory is interested in understanding of the molecular mechanisms that control development of germ cells. These cells belong to an intriguing class of stem cells that give rise to egg and sperm which, after fertilization, generate a zygotic cell. Subsequently, zygotic cell produces all cell types of the next-generation organism. During our research, we are asking one of the most fundamental questions of cell biology: what is responsible for generation of a particular cell type? Specifically, we focus on the comprehensive structure-function ananlysis of the RNA and protein molecules that are crucial for germ cell development. Our laboratory utilizes a wide array of genetic and biochemical approaches, including analysis of mutants, proteomics, and functional in vitro assays using purified components. Please visit our Publications page to view the results of our ongoing research.

近期论文

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1. Zheng J., Gao M, Huynh N, Tindell S.J., Vo H.D.L, McDonald W.H. and Arkov A.L. In vivo mapping of a dynamic ribonucleoprotein granule interactome in early Drosophila embryos. FEBS Open Bio, 6: 1248–1256 (2016). 2. Gao M., Thomson T.C., Creed T.M., Tu S., Loganathan S.N., Jackson C.A., McCluskey P., Lin Y., Collier S.E., Weng Z., Lasko P., Ohi M.D. and Arkov A.L. Glycolytic enzymes localize to ribonucleoprotein granules in Drosophila germ cells, bind Tudor and protect from transposable elements. EMBO Reports, 16: 379-386 (2015). 3. Gao M., McCluskey P., Loganathan S.N., and Arkov A.L. An in vivo crosslinking approach to isolate protein complexes from Drosophila embryos. Journal of Visualized Experiments, Apr 23; (86). doi: 10.3791/51387 (2014). 4. Gao M. and Arkov A.L. Germ cell formation in posterior of early Drosophila embryo. Molecular Reproduction and Development, 80: 589 (2013). 5. Gao M. and Arkov A.L. Next generation organelles: Structure and role of germ granules in the germline. Molecular Reproduction and Development, 80: 610-623 (2013). 6. Creed T.M., Loganathan S.N., Varonin D., Jackson C.A. and Arkov A.L. Novel role of specific Tudor domains in Tudor-Aubergine protein complex assembly and distribution during Drosophila oogenesis. Biochemical and Biophysical Research Communications, 402: 384-389 (2010). 7. Arkov A.L. and Ramos A. Building RNA-protein granules: insight from the germline. Trends in Cell Biology, 20: 482-490 (2010). 8. Thomson T., Liu N., Arkov A., Lehmann R. and Lasko P. Isolation of new polar granule components in Drosophila reveals P body and ER associated proteins. Mechanisms of Development, 125: 865-873 (2008). 9. Arkov A.L., Wang J.Y.S., Ramos A. and Lehmann R. The role of Tudor domains in germline development and polar granule architecture. Development, 133: 4053-4062 (2006). 10. Arkov A.L., Hedenstierna K.O.F and Murgola E.J. Mutational evidence for a functional connection between two domains of 23S rRNA in translation termination. Journal of Bacteriology, 184: 5052-5057 (2002). 11. Murgola E.J., Arkov A.L., Chernyaeva N.S., Hedenstierna K.O.F. and Pagel F.T. rRNA functional sites and structures for peptide chain termination. In R. A. Garrett, S. R. Douthwaite, A. Liljas, A. T. Matheson, P. B. Moore, H. F. Noller (ed.), The Ribosome: structure, function, antibiotics and cellular interactions. ASM Press, Washington, D.C. pp. 509-518 (2000). 12. Arkov A.L., Freistroffer D.V., Pavlov M.Yu., Ehrenberg M. and Murgola E.J. Mutations in conserved regions of ribosomal RNAs decrease the productive association of peptide-chain release factors with the ribosome during translation termination. Biochimie, 82: 671-682 (2000). 13. Arkov A.L. and Murgola E.J. Ribosomal RNAs in translation termination: facts and hypotheses. Biochemistry (Moscow), 64: 1354-1359 (1999). 14. Arkov, A.L., Freistroffer, D.V., Ehrenberg, M. and Murgola, E.J. Mutations in RNAs of both ribosomal subunits cause defects in translation termination. EMBO Journal, 17: 1507-1514 (1998). 15. Arkov, A.L., Mankin, A. and Murgola, E.J. An rRNA fragment and its antisense can alter decoding of genetic information. Journal of Bacteriology,180: 2744-2748 (1998). 16. Murgola, E.J., Pagel, F.T., Hijazi, K.A., Arkov, A.L., Xu, W. and Zhao, S. Q. Variety of nonsense suppressor phenotypes associated with mutational changes at conserved sites in Escherichia coli ribosomal RNA. Biochem. Cell Biol., 73: 925-931 (1995). 17. Arkov, A.L., Korolev, S.V. and Kisselev, L.L. 5’ Contexts of Escherichia coli and human termination codons are similar. Nucleic Acids Research, 23: 4712-4716 (1995). 18. Arkov, A.L., Korolev, S.V. and Kisselev, L. L. Termination of translation in bacteria may be modulated via specific interaction between peptide chain release factor and the last peptidyl tRNA(Ser/Phe). Nucleic Acids Research, 21: 2891-2897 (1993).