Harris, ‌T. K. - University of Miami - Department of Chemistry

个人简介

Ph. D. 1994, University of Mississippi Medical Center Postdoctorate (1994-2000), Johns Hopkins University School of Medicine

研究领域

Mechanistic enzymology, protein engineering, protein NMR, and drug discovery

近期论文

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Horn, D., Zhou, W., Trevisson, E., Al-Ali, H., Harris, T. K., Salviati, L., Barrientos, A. (2010) The conserved mitochondrial twin Cx9C protein Cmc2 is a Cmc1 homolog essential for cytochrome c oxidase biogenesis. J. Biol. Chem. 285, 15088-15099. Al-Ali, H., Rieger, M. E., Seldeen, K. L., Harris, T. K., Farooq, A., and Briegel, K. J. (2010) Biophysical characterization reveals structural disorder in the developmental transcriptional regulator LBH. Biochem. Biophys. Res. Comm. 391, 1104-1109. Harris, T. K., and Keshwani, M. M. (2009) Measurement of enzyme activity.Methods Enzymol. 463, 57-71. Keshwani, M. M., Gao, X., and Harris, T. K. (2009) Mechanism of PDK1-catalyzed T229 phosphorylation of the S6K1 protein kinase. J. Biol. Chem. 284, 22611-22624. Keshwani, M. M., and Harris, T. K. (2008) Kinetic mechanism of fully activated S6K1 protein kinase. J. Biol. Chem. 283, 11972-11980. Keshwani, M. M., Ross, D. B., Ragan, T. J., and Harris, T. K. (2008) Baculovirus-mediated expression, purification, and characterization of a fully activated catalytic kinase domain of the 70 kDa 40S ribosomal protein S6 kinase-1 aII isoform (S6K1aII). Protein Expr. Purif. 58, 32-41. Ragan, T. J., Ross, D., Keshwani, M., and Harris, T. K. (2008) Expression, purification, and characterization of a structurally disordered but functional C-terminal autoinhibitory domain (AID) of the 70 kDa 40S ribosomal protein S6 kinase-1 (S6K1). Protein Expr. Purif. 57, 271-279. Yildiz, I., Gao, X., Harris, T. K., and Raymo, F. M. (2007) Fluoresence resonance energy transfer in quantum dot-protein kinase assemblies. J. Biomed. Biotech. 2007, 18081. Al-Ali, H., Ragan, T. J., Gao, X., and Harris, T. K. (2007) Reconstitution of modular PDK1 functions on trans-splicing of the regulatory PH and catalytic kinase domains. Bioconjugate Chem. 18, 1294-1302. Harris, T. K. (2006) Discovering new drug targeting sites on flexible multi-domain protein kinases: combining segmental isotopic and site-directed spin labeling for NMR detection of interfacial clefts. Methods Mol. Biol. 316, 199-225. Gao, X., and Harris, T. K. (2006) Steady-state kinetic mechanism of PDK1. J. Biol. Chem. 281, 21670-21681. Gao, X., and Harris, T. K. (2006) Role of PH domain in regulating in vitro autophosphorylation events required for reconstitution of PDK1 catalytic activity.Bioorg. Chem. 34, 200-223. Gao, X., Yo, P., and Harris, T. K. (2005) Improved yields for baculovirus-mediated expression of human His6-PDK1 and His6-PKBb/Akt2 and characterization of phospho-specific isoforms for design of inhibitors that stabilize inactive conformations. Protein Expr. Purif. 43, 44-56.