研究领域

Molecular reactivity in complex systems.

The properties of a given molecule or nanoscale structure are conventionally characterized by its mean value that is averaged over a great number of molecules (N~1023). Such an ensemble-averaged description becomes inadequate, however, as the number of molecules under consideration decreases where the observable range of fluctuation approximately scales with N-1/2. To an observer in the macroscopic world, the changes in the microscopic states as a function of time and location are no longer deterministic but probabilistic. The central premise of the current research thrust is that these local stochastic fluctuations give rise to the chemical and physical transformations as they appear in the macroscopic world. This general problem is studied at the most fundamental, single-molecule and single-particle level. New experimental approaches are devised to enable real-time observations of individual molecules as they move and function. Theoretical frameworks are concurrently formulated to afford a quantitative understanding. Current research topics include: (1) High-resolution quantitative single-molecule protein dynamics, where we focus on large-amplitude conformational transitions in proteins and attempt to develop a predictive understanding of these molecular machines. (2) Real-time 3D single-particle tracking spectroscopy, where we are developing new techniques to follow the reactivity of a molecule-scale probe in real time and to directly correlate the chemical or physical processes with the 3D spatial location of the probe.

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"Discovery of protein- and DNA-imperceptible nanoparticle hard coating using gel-based reaction tuning," K. Welsher, S. A. McManus, C.-H. Hsia, S. Yin, H. Yang, J. Am. Chem. Soc., 137, 580-583 (2015). "Kinetics profiling of gramicidin S synthetase A, a member of nonribosomal peptide synthetases," X. Sun, H. Li, J. Alfermann, H. Mootz, H. Yang, Biochemistry, 53, 7983-7989 (2014). "The 'plug-and-go' strategy to manipulate streptavidin valencies," X. Sun, D. Montiel, H. Li, and H. Yang, Bioconjugate Chem., 25, 1375-1380 (2014). "Stochastic Localization of Micro-Swimmers by Photon Nudging," A. Bregulla, H. Yang, F. Cichos, ACS Nano, 8, 6542-6550 (2014). "Analysis of Trajectory Entropy for Continuous Stochastic Processes at Equilibrium," K. R. Haas, H. Yang, and J.-W. Chu, J. Phys. Chem. B, 118, 8099-8107 (2014). "The Trajectory Entropy of Continuous Stochastic Processes at Equilibrium," K. R. Haas, H. Yang, and J.-W. Chu, J. Phys. Chem. Lett., 5, 999-1003 (2014). "Mechanodelivery of Nanoparticles to the Cytoplasm of Living Cells," N. T. Emerson, C.-H. Hsia, I. U. Rafalska-Metcalf, and H. Yang, Nanoscale, 6, 4538-4543 (2014). "Multi-resolution 3D Visualisation of the Early Stages of Cellular Uptake of Peptide-coated Nanoparticles," K. Welsher and H. Yang, Nat. Nanotechnol., 9, 198-203 (2014). "Fisher Information Metric for the Langevin Equation and Least Informative Models of Continuous Stochastic Dynamics," K. R. Haas, H. Yang, and J.-W. Chu, J. Chem. Phys., 139, 121931 (2013). "Expectation-Maximization of the Potential of Mean Force and Diffusion Coefficient in Langevin Dynamics from Single-Molecule FRET Data Photon by Photon," K. R. Haas, H. Yang, and J.-W. Chu, J. Phys. Chem. B, 117, 15591-15605 (2013). "Numerical Construction of Estimators for Single-Molecule Fluorescence Measurements," S. Kawai, D. Cooper, C. Landes, H. D. Mootz, H. Yang, and T. Komatsuzaki, in J. Phys. Chem. B, 117, 8061-8074 (2013). "Confocal Single-Molecule FRET for Protein Conformational Dynamics," Y.-W. Tan, J. A. Hanson, J.-W. Chu, and H. Yang, in Protein Dynamics: Methods and Protocols in the Methods in Molecular Biology series, vol. 1084, Ed. Dennis R. Livesay, September 30 (2013). "Harnessing Thermal Fluctuations for Purposeful Activities: The Manipulation of Single Micro-swimmers by Adaptive Photon Nudging," B. Qian, D. Montiel, A. Bregulla, F. Cichos, and H. Yang, to appear in Chem. Sci. 4, 1420-1429 (2013). (Cover Story) "Ligand Synthesis and Passivation for Silver and Large Gold Nanoparticles for Single-Particle-Based Sensing and Spectroscopy," D. Montiel, E. V. Yates, L. Sun, M. M. Sampias, J. Malona, E. J. Sorensen, and H. Yang, in Nanomaterial Interfaces in Biology: Methods and Protocols in the Methods in Molecular Biology series, vol. 1025, Eds. Kimberly Hamad-Schifferli and Paolo Bergese, August 31 (2013). "Quantitative in vitro Biochemistry, one molecule at a time," J. A. Hanson and H. Yang, in Quantitative Biology: From Molecular to Cellular Systems, Edited by Michael E. Wall, Taylor and Francis Group, August (2012). "Structural Distributions from Single-Molecule Measurements as a Tool for Molecular Mechanics," J. A. Hanson, J. Brokaw, C. C. Hayden, J.-W. Chu, and H. Yang, Chem. Phys., 396, 61-67 (2012). "Model-Free Analysis of Time-Dependent Single-Molecule Spectroscopy," K. Welsher and H. Yang, Proceedings of the 9th IEEE International Symposium on Biomedical Imaging, pp. 921-923, Barcelona, Spain, May 2012. "An Accessible Approach to Preparing Water-Soluble Mn2+-doped (CdSSe)ZnS (Core)Shell Nanocrystals for Ratiometric Temperature Sensing," C.-H. Hsia, A. Wuttig, and H. Yang, ACS Nano, 5, 9511-9522 (2011). "Quantum Dot Nano Thermometers Reveal Heterogeneous Local Thermogenesis in Living Cells," J.-M. Yang, H. Yang, and L. Lin, ACS Nano, 5, 5067 (2011). "Seeing the Forest for the Trees: Fluorescence Studies of Single Enzymes in the Context of Ensemble Experiments," Y.-W. Tan and H. Yang, Phys. Chem. Chem. Phys., 13, 1709 (2011).