Koculi, Eda - University of Central Florida

个人简介

Education: May 2006: Doctor of Philosophy in Biophysics, The Johns Hopkins University. May 1999: Master of Science in Biochemistry, University of Oklahoma. Aug. 1996: Bachelor of Science in Chemistry with High Honors, Illinois Institute of Technology. Research Experience: Aug. 2013 - Present: Assistant Professor. Department of Chemistry, University of Central Florida. Feb. 2011- Jun. 2013: Research Professional. Laboratory of Prof. Lucia Rothman-Denes, University of Chicago. Nov. 2008 -Aug. 2010: Postdoctoral fellow. Laboratory of Prof. Olke Uhlenbeck, Northwestern University. Apr. 2005 - Oct. 2008: Postdoctoral fellow. Laboratory of Prof. Arthur Horwich, The Scripps Research Institute and Howard Hughes Medical Institute Yale University School of Medicine. May 2001 - Apr. 2005: Graduate Research Assistant. Laboratory of Prof. Sarah Woodson, The Johns Hopkins University Jun. 1999 - Aug. 2000: Research Assistant. Advanced Center for Genome Technology directed by Prof. Bruce Roe, University of Oklahoma. Jan. 1997 - May 1999: Graduate Research Assistant. Advanced Center for Genome Technology directed by Prof. Bruce Roe, University of Oklahoma. Awards: Feb. 2015: Research Travel Grant, University of Central Florida May 2014: In House Grant, University of Central Florida Jun. 2010: Winner of the Young Investigator Poster Competition at the Biopolymer Gordon Conference, Newport, RI Jun. 2010: Interdepartmental Biological Sciences Postdoctoral Travel Award, Northwestern University, Evanston, IL Mar. 2010: Advancing Biomedical Research Workforce Diversity: NIGMS Workshop for Postdocs Transitioning to Independent Positions, Bethesda, MD Mar. 2007 - Oct. 2008: Ruth L. Kirschstein National Research Service Award, National Institutes of Health Mar. 2002: J. Brein Key Graduate Student Assistant Fund, The Johns Hopkins University, Baltimore, MD Aug. 1994 - May 1995: Energy Environment Economy Scholarship, Illinois Institute of Technology, Chicago, IL Aug. 1993 - May 1994: Flood Scholarship, Illinois Institute of Technology, Chicago, IL

研究领域

The research in the Koculi laboratory aims to investigate RNA-protein interactions to understand fundamental biological processes implicated in HIV-1 infection, cancer progression, and bacterial survival. Student and postdoctoral fellows in our laboratory are engaged in multidisciplinary research that combines molecular biology, chemistry, biochemistry and biophysics. The research in our laboratory can be divided in two areas. Regulation of DDX3 Catalytic Activity DDX3 is a human DEAD-box protein that is essential for the export of partially spliced and unspliced HIV-1 transcripts from the nucleus to the cytoplasm, and it is an important regulator of breast cancer metastasis. To design specific inhibitors of DDX3, a molecular understanding is required of how the DDX3 function is regulated. At present, knowledge of this regulatory mechanism is completely missing and it will be one of the major aims of our research. RNA Intermediate Structures on Ribosome Assembly The structural rearrangements that DEAD-box proteins perform during large subunit ribosome assembly are not known. The determination of these structural rearrangements will provide insight into the ribosome assembly process and inform the development of new classes of antibiotics that target the large ribosome subunit assembly pathway. In the present climate of increasingly antibiotic-resistant pathogenic bacteria, the discovery of new antibiotic targets is of paramount importance.

近期论文

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Koculi, E., Cho, S. S., Desai, R., Thirumalai, D., Woodson, S. “Folding of P5abc RNA involves direct coupling of secondary and tertiary structures.” Nucleic Acids Res. 40: 8001-8020, 2012 Koculi, E., Horst, R., Horwich, A.L., Wuthrich, K. “Nuclear magnetic resonance spectroscopy with the stringent substrate rhodanese bound to the single-ring variant SR1 of the E. coli chaperonin GroEL.” Protein Sci. 20:1380-1386, 2011 Koculi, E. and Woodson, S. “Analysis of RNA Folding by Native Polyacrylamide Gel Electrophoresis.” Methods in Enzymology 469:189-208, 2009 Pedrini B., Placzek W.J., Koculi, E., Alimenti C., LaTerza A., Luporini P., Wüthrich K. “Cold-adaptation in sea-water-borne signal proteins: sequence and NMR structure of the pheromone En-6 from the Antarctic ciliate Euplotes nobilii.” J. Mol. Biol. 372: 277-286, 2007 Koculi, E., Hyeon C., Thirumalai D., Woodson S. “Charge density of divalent metal cations determines RNA stability.” J.Am.Chem. 129: 2676-2682, 2007 Koculi, E., Thirumalai, D. and Woodson, S. “Counterion charge density determines the position and plasticity of RNA folding transition states.” J.Mol. Biol. 359: 446-454, 2006 Koculi, E., Lee, N., Thirumalai, D. and Woodson, S. “Folding of Tetrahymena ribozyme by polyamines: importance of counterion valence and size.” J.Mol. Biol. 341: 27-36, 2004 Chernova, O.B., Hundyadi, A., Malaj, E., Pan, H., Crooks, C., Roe, B., Cowell, J.K. “A novel member of the WD-repeat gene family, WDR11, maps to the 10q26 region and is disrupted by a chromosome translocation in human glioblastoma cells.” Oncogene 20: 5378-5392, 2001 Lander, E.S. et al. “Initial sequencing and analysis of the human genome.” Nature 409: 860-921, 2001 Kitimura, E., Su, G., Sossey-Alaoui, K., Malaj, E., Lewis, J., Pan, H., Hawthorn, L.,Roe, B., Cowell, J.K. “A transcription map of the minimally deleted region from 13q14 in B- 3cell chronic lymphocytic leukemia as defined by large scale sequencing of the 650 kb critical region.” Oncogene 19:5772-5780, 2000 Pletcher, M.T., Roe, B., Chen, F., Do, T., Do, A., Malaj, E., Reeves, R.H. “Chromosome evolution: the junction of mammalian chromosomes in the formation of mouse chromosome 10.” Genome Res. 10:1463-1467, 2000 Dunham, I. et.al. “The DNA Sequence of Human Chromosome 22.” Nature 402: 489-485, 1999