陈知行 - 北京大学 - 分子医学研究所

个人简介

2004-2008 BS in Chemical Biology, Tsinghua University 2008-2009 Research Assistant, Peking University 2009-2014 PhD in Chemistry, Columbia University 2015 Postdoc Researcher, Columbia University 2016-2018 Postdoc Researcher, Stanford University 2018- Assistant Professor, Peking University Dr. Zhixing Chen is a chemist with research experience spanning natural product synthesis, polymer mechano-chemistry, fluorescence and non-linear optical probes, bioconjugation, and live cell imaging. Zhixing believes in the enabling role of molecular technologies at the frontier of optical imaging, biotechnology, and life sciences. His current focus is to develop small and macro molecular tools for advanced imaging technologies. In the long term, Zhixing endeavors to unleash the synergy at the chemistry-biology-imaging interface in a collaborative manner, bringing new dimensions to the fields of molecular imaging and molecular medicine.

研究领域

As an emerging yet interdisciplinary field, bioimaging is revolutionizing biological and medical research. Trained as a general chemist, Dr. Chen has been approaching this field from a molecular aspect. Our lab endeavors to develop novel molecular tools by integrating the essence of chemical synthesis, spectroscopy, polymer, and chemical biology. The molecular tools would be applied as cutting-edge technologies to various fields including biophysics, cell biology, physiology, and finally inspire drug discovery. Our research philosophy features: 1. based on chemical principles, we customize tailored molecules as enabling solutions; 2. we leverage the platforms of IMM and CLS as unique opportunities to bring modern molecular tools to the frontiers of biological sciences and molecular medicine. We believe our training in chemistry and biotechnology, our cross-disciplinary experience, and our problem-solving mindset would broaden the career path of trainees.

近期论文

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Chen, J.; Stephan, T.; Gaedke, F.; Liu, T.; Li, Y. Schauss, A.; Chen, P.; Wulff, V.; Jakobs, S.; Jüngst, C.*; Chen, Z.* An aldehyde-crosslinking mitochondrial probe for STED imaging in fixed cells. PNAS 2024, 121, e2317703121. Zhang, Y.; Yang, Z.; Dilizhatai, S.; Shen, X.; Ye, J.; Yu, B.; Pefaur, N.; Scheer, J. M.; Nixon, A. E.*; Chen, Z.* Geometric Antibody Engineering Reveals the Spatial Factor on the Efficacy of Bispecific T Cell Engagers. ACS Chem. Biol. 2024, 19, 916-25 Zhang, Y.;Ling, J.; Liu, T.; Chen, Z.* Lumos maxima – How robust fluorophores resist photobleaching? Curr. Opin. Chem. Biol. 2024, 79, 102439 Peng, X.#; Ren, H.#; Yang, L.#; Tong, S.#; et al.; Chen, Z.; Liu, H.*; Chen, L*. Readily releasable β cells with tight Ca2+–exocytosis coupling dictate biphasic glucose-stimulated insulin secretion. Nat. Metablism 2024, 6, 238-53 Li, C.#; Cheng, K.#; Zhao, Q.#; et al.; Quan, L.*; Chen, Z.* Diazo-carboxyl Click Derivatization Enables Sensitive Analysis of Carboxylic Acid Metabolites in Biosamples. Anal. Chem. 2023, 95, 16976. Dilizhatai, S.; Chen, Z.* Chemical tags and beyond: Live-cell protein labeling technologies for modern optical imaging. Smart Molecules. 2023, 1, e20230002. Liu, S.#; Ling, J.#; et al.; Zou, P.*; Chen, Z.* Orange/far-red hybrid voltage indicators with reduced phototoxicity enable reliable long-term imaging in neurons and cardiomyocytes. PNAS 2023, 120, e2306950120. Yang, X.; Chen, D.; Sun, Q. et al.; Chen, Z.; Liu, Y.*; Zhang, J.*; Zhao, Y.* A live-cell image-based machine learning strategy for reducing variability in PSC differentiation systems. Cell Discov. 2023, 9, 53. Yu, Q.; Yao, Z.; Zhang, H.; Li, Z.; Chen, Z.; Xiong, H.* Transient Stimulated Raman Excited Fluorescence Spectroscopy. J. Am. Chem. Soc. 2023, 145, 7758-62. Zhang, Y.#; Yang, C.#; Peng, S.; Ling, J.; Chen, P.; Ma, Y.; Wang, W.; Chen, Z.*; Chen, C.* General Strategy To Improve the Photon Budget of Thiol-Conjugated Cyanine Dyes. J. Am. Chem. Soc. 2023, 145, 4187-98. Liu, T.#; Stephen, T.#; Chen, P.; Keller-Findeisen, J.; Chen, J.; Riedel, D.; Yang, Z.; Jakobs, S.; Chen, Z.* Multi-color live-cell STED nanoscopy of mitochondria with a gentle inner membrane stain. PNAS 2022, 119, e2215799119 [Cover] Mo, J.#; Chen, J.#; Shi, Y.; Sun, J.; Wu, Y.; Liu, T.; Zhang, J.; Zheng, Y.; Li, Y.; Chen, Z.* Third-Generation Covalent TMP-Tag for Fast Labeling and Multiplexed Imaging of Cellular Proteins. Angew. Chem. Int. Ed. 2022, 61, e202207905 Liu, M.#; Zhang, J.#; Chen, Z.* Emerging trends in fluorescence bioimaging of divalent metal cations using small-molecule indicators. Chem. Eur. J. 2022, 28, e202200587. [Invited Review] Du, J.; Zheng, L.; Gao, P.; Yang, H.; Yang, W.-J.; Guo, F.; Liang, R.; Feng, M.; Wang, Z.; Zhang, Z.; Bai, L.; Bu, Y.; Xing, S.; Zheng, W.; Wang, X.; Quan, L.; Hu, X.; Wu, H.; Chen, Z.; Chen, L.; Wei, K.; Zhang, Z.; Zhu, X.; Zhang, X.; Tu, Q.; Zhao, S.-M.*; Lei, X.*; Xiong, J.-W.* A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration. Cell Stem Cell 2022, 29, 545-58. Niu, Y.; Liu, R.; Guan, C.; Zhang, Y.; Chen, Z.; Hoerer, S.; Nar, H.; Chen, L.* Structural basis of inhibition of the human SGLT2–MAP17 glucose transporter. Nature 2022, 601, 280-4. Zhang, J.#; Peng, X.#; Wu, Y.#; Ren, H.#; Sun, J.; Tong, S.; Liu, T.; Zhao, Y.; Wang, S.; Tang, C.; Chen, L.; Chen, Z.* Red- and Far-Red-Emitting Zinc Probes with Minimal Phototoxicity for Multiplexed Recording of Orchestrated Insulin Secretion. Angew. Chem. Int. Ed. 2021, 60, 25846-55. [Inside Cover] Yang, Z.#; Li, L.#; Ling, J.; Liu, T.; Huang, X.; Ying, Y.; Zhao, Y.; Zhao, Y.; Lei, K.*; Chen, L.*; Chen, Z.* Cyclooctatetraene-conjugated cyanine mitochondrial probes minimize phototoxicity in fluorescence and nanoscopic imaging. Chem. Sci. 2020, 11, 8506-8516. Chen, Z.#; Zhu, X.#; Yang, J.; Mercer, J. A. M.; Burns, N. Z.; Martinez, T. J.; Xia, Y. The cascade unzipping of ladderane reveals dynamic effects in mechanochemistry. Nat. Chem. 2020, 12, 302-309. Su, J. K.; Feist, J. D.; Yang, J.; Mercer, J. A. M.; Romaniuk, J. A. H.; Chen, Z.; Cegelski, L.; Burns, N. Z.; Xia, Y. Synthesis and Mechanochemical Activation of Ladderene–Norbornene Block Copolymers. J. Am. Chem. Soc. 2018, 140, 12388. Shi, L.; Zheng, C.; Shen, Y.; Chen, Z.; Silveira, E. S.; Zhang, L.; Wei, M.; Liu, C.; de Sena-Tomas, C.; Targoff, K.; Min, W. Optical imaging of metabolic dynamics in animals. Nature Communications 2018, 9, 2995. Qiao, Y.; Zhou, Z.; Chen, Z.; Du, S.; Cheng, Q.; Zhai, H.; Fritz, N. J.; Du, Q.; Yang, Y. Visualizing ion diffusion in battery systems by fluorescence microscopy: A case study on the dissolution of LiMn2O4. Nano Energy 2018, 45, 68. Chen, Z.; Mercer, J. A. M.; Zhu, X.; Romaniuk, J. A. H.; Pfattner, R.; Cegelski, L.; Martinez, T. J.; Burns, N. Z.; Xia, Y. Mechanochemical unzipping of insulating polyladderene to semiconducting polyacetylene. Science 2017, 357, 475-479 [Front Cover]. Wei, L.; Chen, Z.; Shi, L.; Long, R.; Anzalone, A. V.; Zhang, L.; Hu, F.; Yuste, R.; Cornish, V. W.; Min, W. Super-multiplex vibrational imaging. Nature 2017, 544, 465-470. Wei, L.; Hu, F.; Chen, Z.; Shen, Y.; Zhang, L.; Min, W., Live-Cell Bioorthogonal Chemical Imaging: Stimulated Raman Scattering Microscopy of Vibrational Probes. Acc. Chem. Res. 2016, 49, 1494-1502 Anzalone, A. V; Chen, Z.; Cornish, V. W. Synthesis of Photoactivatable Azido-acyl Caged Oxazine Fluorophores for Live-cell Imaging. Chem. Commun. (Camb). 2016, 52, 9442-9445 Freyer, J. L.; Brucks, S. D.; Gobieski, G. S.; Russell, S. T.; Yozwiak, C. E.; Sun, M.; Chen, Z.; Jiang, Y.; Bandar, J. S.; Stockwell, B. R.; Lambert, T. H.; Campos, L. M., Clickable Poly(ionic liquids): A Materials Platform for Transfection. Angew. Chem. Int. Ed. 2016, 55, 12382-12386 Hu, F.; Chen, Z.; Zhang, L.; Shen, Y.; Wei, L.; Min, W. Vibrational Imaging of Glucose Uptake Activity in Live Cells and Tissues by Stimulated Raman Scattering. Angew. Chem. Intl. Ed. 2015, 54, 9821-9825 Chen, Z.; Paley, D. W.; Wei, L.; Weisman, A. L.; Friesner, R. A.; Nuckolls, C.; Min, W. Multicolor Live-Cell Chemical Imaging by Isotopically Edited Alkyne Vibrational Palette. J. Am. Chem. Soc. 2014, 136, 8027–8033. Wei, L.; Hu, F.; Shen, Y.; Chen, Z.; Yu, Y.; Lin, C.-C.; Wang, M. C.; Min, W. Live-Cell Imaging of Alkyne-Tagged Small Biomolecules by Stimulated Raman Scattering. Nat. Methods 2014, 11, 410–412.