Douglas, Don - University of British Columbia

个人简介

B.Sc., McMaster University 1971; Ph.D., University of Toronto (J. C. Polanyi, 1976); Post Doctoral Fellow, University of California, Berkeley (C.B. Moore, 1976-1978); Senior and Principal Research Scientist, SCIEX, 1979-1994; Barringer Award of the Spectroscopy Society of Canada, 1988; Fisher Scientific Award of the CIC, 1997, Noranda Award of the CSC 2001, FCIC; Fred Lossing Award of the Canadian Society for Mass Spectrometry, 2001; UBC Killam Research Award, 2003; elected Fellow of the Royal Society of Canada, 2007; NSERC-SCIEX Industrial Research Chair.

研究领域

The last few decades have seen dramatic growth of mass spectrometry into new application areas. Most notable among these have been the applications to biomolecules, particularly peptides and proteins. Research in this group centers on the development of new techniques in mass spectrometry and new instrumentation and for such applications. Electrospray ionization sources can produce gas-phase ions of proteins at atmospheric pressure directly from proteins in solution. The ionization process is sufficiently gentle that proteins up to a molecular weight of at least 500,000 can be ionized. In many cases noncovalent complexes between proteins and other proteins, or between proteins and small molecules, can be observed in mass spectra. Little is known about the structure of these gas-phase ions or complexes. Research here is directed towards new methods and new instrumentation to study the structures of these ions and to determine the relationships of these structures, if any, to those of proteins in solution. Collision cross sections are measured to give an estimate of ion "size". Gas-phase hydrogen/deuterium exchange is used to probe protein conformation, and tandem mass spectrometry is used to determine relative binding strengths of gas-phase noncovalent complexes. This work is done in collaboration with protein chemists in the Department of Chemistry and Department of Biochemistry at UBC. Instrumentation for mass spectrometry continues to evolve rapidly. Work in this lab is directed towards developing improved linear quadrupole ion trap mass spectrometers. A linear quadrupole mass filter is operated at very low resolution and stopping potentials are applied to electrodes at the ends of the quadrupole. As a result, ions with a broad range of mass to charge ratios can be confined in the trap, manipulated, and then ejected in a mass selective manner to produce a mass spectrum. Current work is directed to understanding and reducing so called "space charge" effects in such traps. If too many ions are introduced to a trap, the Coulombic repulsion between the ions ("space charge") changes the character of the ion motion. The net result is changes of the apparent masses of ions and a loss of mass resolution in mass spectra. This work is sponsored through an NSERC-SCIEX Industrial Research Chair. As a result, there are opportunities for consultation and co-operation with researchers at AB SCIEX, a major manufacturer of mass spectrometers located near Toronto.

近期论文

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Douglas, D. J. ; Konenkov, N. V. Mass Resolution Of Linear Quadrupole Ion Traps With Round Rods. RAPID COMMUNICATIONS IN MASS SPECTROMETRY 2014, 28, 2252-2258.BibTex Douglas, D. J. ; Konenkov, N. V. Mass Selectivity Of Dipolar Resonant Excitation In A Linear Quadrupole Ion Trap. RAPID COMMUNICATIONS IN MASS SPECTROMETRY 2014, 28, 430-438.BibTex Douglas, D. J. ; Polyakov, A. ; Konenkov, N. V. Quadrupolar Ion Excitation For Radiofrequency-Only Mass Filter Operation. EUROPEAN JOURNAL OF MASS SPECTROMETRY 2014, 20, 207-214.BibTex 2013 Rajabi, K. ; Douglas, D. J. The Effect Of A Covalent And A Noncovalent Small-Molecule Inhibitor On The Structure Of Abg Beta-Glucosidase In The Gas Phase. Journal of the American Society for Mass Spectrometry 2013, 24, 907-916.BibTex Gao, C. ; Douglas, D. J. Can The Effective Potential Of A Linear Quadrupole Be Extended To Values Of The Mathieu Parameter Q Up To 0.90?. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY 2013, 24, 1848-1852.BibTex 2012 Douglas, D. J. ; Konenkov, N. V. Ion Cloud Model For A Linear Quadrupole Ion Trap. European Journal of Mass Spectrometry 2012, 18, 419-429.BibTex Kang, Y. ; Terrier, P. ; Ding, C. ; Douglas, D. J. Solution And Gas-Phase H/d Exchange Of Protein Small-Molecule Complexes: Cex And Its Inhibitors. Journal of the American Society for Mass Spectrometry 2012, 23, 57-67.BibTex Douglas, D. J. ; Konenkov, N. V. Trajectory Calculations Of Space-Charge Induced Mass Shifts In A Linear Quadrupole Ion Trap. Rapid Communications in Mass Spectrometry 2012, 26, 2105-2114.BibTex 2011 Kang, Y. ; Douglas, D. J. Gas-Phase Ions Of Human Hemoglobin A, F, And S. Journal of The American Society for Mass Spectrometry 2011, 22, 1187 - 1196.BibTex Kang, Y. ; Terrier, P. ; Douglas, D. J. Mass Spectra And Ion Collision Cross Sections Of Hemoglobin. Journal of The American Society for Mass Spectrometry 2011, 22, 290 - 299.BibTex Gao, C. ; Mao, D. ; Konenkov, N. ; Douglas, D. J. Space Charge Effects With Mass Selective Axial Ejection From A Linear Quadrupole Ion Trap. Rapid Communications in Mass Spectrometry 2011, 25, 3509-3520.BibTex 2010 Terrier, P. ; Douglas, D. J. Carbamino Group Formation With Peptides And Proteins Studied By Mass Spectrometry. Journal of the American Society for Mass Spectrometry 2010, 21, 1500 - 1505.BibTex Zhao, X. Zhen; Xiao, Z. ; Douglas, D. J. Mass Analysis With Islands Of Stability With Linear Quadrupoles Incorporating Higher Order Multipole Fields. Journal of the American Society for Mass Spectrometry 2010, 21, 393 - 402.BibTex Xiao, Z. ; Zhao, X. - Z. ; Douglas, D. J. Quadrupole Mass Filters With Added Hexapole Fields. Rapid Communications in Mass Spectrometry 2010, 24, 1985 - 1992.BibTex 2009 Douglas, D. J. Linear Quadrupoles In Mass Spectrometry. Mass Spectrometry Reviews 2009, 28, 937-960.BibTex Wright, P. J. ; Douglas, D. J. Gas-Phase H/d Exchange And Collision Cross Sections Of Hemoglobin Monomers, Dimers, And Tetramers. Journal of the American Society for Mass Spectrometry 2009, 20, 484-495.BibTex Zhao, X. Z. ; Xiao, Z. L. ; Douglas, D. J. Overcoming Field Imperfections Of Quadrupole Mass Filters With Mass Analysis In Islands Of Stability. Analytical Chemistry 2009, 81, 5806-5811.BibTex 2008 Moradian, A. ; Douglas, D. J. Mass Selective Axial Ion Ejection From Linear Quadrupoles With Added Octopole Fields. Journal of the American Society for Mass Spectrometry 2008, 19, 270-280.BibTex Wright, P. J. ; Zhang, J. M. ; Douglas, D. J. Conformations Of Gas-Phase Ions Of Ubiquitin, Cytochrome C, Apomyoglobin, And Beta-Lactoglobulin Produced From Two Different Solution Conformations. Journal of the American Society for Mass Spectrometry 2008, 19, 1906-1913.BibTex Zhao, X. Z. ; Douglas, D. J. Dipole Excitation Of Ions In Linear Radio Frequency Quadrupole Ion Traps With Added Multipole Fields. International Journal of Mass Spectrometry 2008, 275, 91-103.BibTex Zhao, X. Z. ; Granot, O. ; Douglas, D. J. Quadrupole Excitation Of Ions In Linear Quadrupole Ion Traps With Added Octopole Fields. Journal of the American Society for Mass Spectrometry 2008, 19, 510-519.BibTex 2007 Konenkov, N. ; Zhao, X. Z. ; Xiao, Z. L. ; Douglas, D. J. Mass Analysis In Islands Of Stability With Linear Quadrupoles With Added Octopole Fields. Journal of the American Society for Mass Spectrometry 2007, 18, 826-834.BibTex Tesic, M. ; Wicki, J. ; Poon, D. K. Y. ; Withers, S. G. ; Douglas, D. J. Gas Phase Noncovalent Protein Complexes That Retain Solution Binding Properties: Binding Of Xylobiose Inhibitors To The Beta-1,4 Exoglucanase From Cellulomonas Fimi. Journal of the American Society for Mass Spectrometry 2007, 18, 64-73.BibTex Moradian, A. ; Douglas, D. J. Experimental Investigation Of Mass Analysis Using An Island Of Stability With A Quadrupole With 2.0% Added Octopole Field. Rapid Communications in Mass Spectrometry 2007, 21, 3306-3310.BibTex