个人简介

B.Sc, St. Mary's University (1976); PhD. Alberta(G. Horlick 1981); Postdoctoral, Indiana (G.M. Hieftje, 1980-81); W.A.E. McBryde Medal, Canadian Society for Chemistry (1987); U.B.C. Killam Research Prize (1988-89), Killam Senior Fellowship (1991-92); Fisher Scientific Lecture Award, Canadian Society for Chemistry(1994); Royal Society of Chemistry Award for Analytical Spectroscopy (1996); Smiths Detection Spectroscopy Award, Canadian Spectroscopy Society (2004). Fellow of the Society for Applied Spectroscopy (2009, Editor for Applied Spectroscopy (2009 -). Editorial Board - Analytical Chemistry (2010 -)

研究领域

Analytical Chemistry

Dr. Blades' research interests involve the development, characterization, and application of optical and mass spectroscopic methods for chemical analysis. Bioanalytical Raman Spectroscopy - A collaboration with Professor Robin Turner The ability of ultraviolet resonance Raman spectroscopy (UVRRS) to determine structural and environmental information concerning biomolecules at low concentration in aqueous solution make it a very powerful bioanalytical and biophysical technique. Unfortunately, its utility has been limited due to experimental sampling requirements which make in-situ or in-vivo studies difficult. In a collaborative project with Professor Robin Turner (Biotechnology, U.B.C.) the performance of fiber-optic UVRRS probes are being investigated using novel for optimizing light delivery and collection efficiency using solarization resistant UV fibers. UVRRS is being used for the study of the structure and properties of proteins and peptides, DNA, and for the in-vivo detection of small molecule neurotransmitters. Atmospheric Pressure Photoionization Mass Spectrometry Our group is studying and developing atmospheric pressure ionization (API) sources for interfacing liquid chromatography (LC) with mass spectrometry (MS). Electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) are the most commonly used API sources for LC-MS, and they are applied for the analysis of molecular species in a wide range of settings, most notably the pharmaceutical industry. There exist, however, important classes of compounds, such as steroids, which are not ionized with adequate efficiency by ESI and APCI. In an effort to expand the range of compounds amenable to LC-MS, as well as to improve the sensitivity of LC-MS methods in general, a new ionization method, atmospheric pressure photoionization (APPI), has been recently developed (Robb et al., Anal. Chem. 2000, 72, 3653-3659). APPI has since been commercialized by MDS-SCIEX as the "PhotoSpray" ion source, and it is now utilized in a diverse range of LC- MS applications. One of our ongoing research projects is aimed at elucidating the fundamental mechanisms responsible for the performance of the APPI source. As these mechanisms become better understood, ideas for improvements to the source are born. Future research projects will be undertaken to investigate improvements to the APPI source, as well as to develop additional novel API sources.

近期论文

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Konorov, S. O. ; H. Schulze, G. ; Piret, J. M. ; Blades, M. W. ; Turner, R. F. B. Label-Free Determination Of The Cell Cycle Phase In Human Embryonic Stem Cells By Raman Microspectroscopy. ANALYTICAL CHEMISTRY 2013, 85, 8996-9002.BibTex H. Schulze, G. ; Konorov, S. O. ; Piret, J. M. ; Blades, M. W. ; Turner, R. F. B. Label-Free Imaging Of Mammalian Cell Nucleoli By Raman Microspectroscopy. ANALYST 2013, 138, 3416-3423.BibTex 2012 Robb, D. B. ; Rogalski, J. C. ; Kast, J. ; Blades, M. W. Liquid Chromatography-Atmospheric Pressure Electron Capture Dissociation Mass Spectrometry For The Structural Analysis Of Peptides And Proteins. ANALYTICAL CHEMISTRY 2012, 84, 4221-4226.BibTex H. Schulze, G. ; Konorov, S. O. ; Okuda, K. ; Piret, J. M. ; Blades, M. W. ; Turner, R. F. B. A Volume-Exclusion Normalization Procedure For Quantitative Raman Confocal Microspectroscopy Of Immersed Samples Applied To Human Embryonic Stem Cells. JOURNAL OF RAMAN SPECTROSCOPY 2012, 43, 360-369.BibTex Konorov, S. O. ; Jardon, M. A. ; Piret, J. M. ; Blades, M. W. ; Turner, R. F. B. Raman Microspectroscopy Of Live Cells Under Autophagy-Inducing Conditions. ANALYST 2012, 137, 4662-4668.BibTex Tan, Y. ; Konorov, S. O. ; H. Schulze, G. ; Piret, J. M. ; Blades, M. W. ; Turner, R. F. B. Comparative Study Using Raman Microspectroscopy Reveals Spectral Signatures Of Human Induced Pluripotent Cells More Closely Resemble Those From Human Embryonic Stem Cells Than Those From Differentiated Cells. ANALYST 2012, 137, 4509-4515.BibTex 2011 Konorov, S. O. ; Blades, M. W. ; Turner, R. F. B. Non-Resonant Background Suppression By Destructive Interference In Coherent Anti-Stokes Raman Scattering Spectroscopy. Optics Express 2011, 19, 25925.BibTex Konorov, S. O. ; H. Schulze, G. ; Atkins, C. G. ; Piret, J. M. ; Aparicio, S. A. ; Turner, R. F. B. ; Blades, M. W. Absolute Quantification Of Intracellular Glycogen Content In Human Embryonic Stem Cells With Raman Microspectroscopy. Analytical Chemistry 2011, 83, 6254 - 6258.BibTex H. SCHULZE, G. E. O. R. G. ; Konorov, S. O. ; Aparicio, S. A. ; Piret, J. M. ; Blades, M. W. ; Turner, R. F. B. Raman Microscopy Of Human Embryonic Stem Cells Exposed To Heat And Cold Stress. Applied Spectroscopy 2011, 65, 1380 - 1386.BibTex Konorov, S. O. ; H. Schulze, G. ; Piret, J. M. ; Turner, R. F. B. ; Blades, M. W. Evidence Of Marked Glycogen Variations In The Characteristic Raman Signatures Of Human Embryonic Stem Cells. Journal of Raman Spectroscopy 2011, 42, 1135 - 1141.BibTex Robb, D. B. ; Rogalski, J. C. ; Kast, J. ; Blades, M. W. A New Ion Source And Procedures For Atmospheric Pressure-Electron Capture Dissociation Of Peptides. Journal of The American Society for Mass Spectrometry 2011, 22, 1699 - 1706.BibTex Konorov, S. O. ; H. Schulze, G. ; Caron, N. J. ; Piret, J. M. ; Blades, M. W. ; Turner, R. F. B. Raman Microspectroscopic Evidence That Dry-Fixing Preserves The Temporal Pattern Of Non-Specific Differentiation In Live Human Embryonic Stem Cells. Journal of Raman Spectroscopy 2011, 42, 576 - 579.BibTex Addison, C. J. ; Konorov, S. O. ; H. Schulze, G. ; Turner, R. F. B. ; Blades, M. W. Residual Benzamide Contamination In Synthetic Oligonucleotides Observed Using Uv Resonance Raman Spectroscopy. Journal of Raman Spectroscopy 2011, 42, 349 - 354.BibTex Robb, D. B. ; Rogalski, J. C. ; Kast, J. ; Blades, M. W. A New Ion Source And Procedures For Atmospheric Pressure-Electron Capture Dissociation Of Peptides. Journal of The American Society for Mass Spectrometry 2011.BibTex 2010 Robb, D. B. ; Rogalski, J. C. ; Kast, J. ; Blades, M. W. Atmospheric Pressure-Electron Capture Dissociation Of Peptides Using A Modified Photospray Ion Source. Rapid Communications in Mass Spectrometry 2010, 24, 3303 - 3308.BibTex Konorov, S. O. ; Blades, M. W. ; Turner, R. F. B. Lorentzian Amplitude And Phase Pulse Shaping For Nonresonant Background Suppression And Enhanced Spectral Resolution In Coherent Anti-Stokes Raman Scattering Spectroscopy And Microscopy. Applied Spectroscopy 2010, 64, 767 - 774.BibTex H. Schulze, G. ; Konorov, S. O. ; Caron, N. J. ; Piret, J. M. ; Blades, M. W. ; Turner, R. F. B. Assessing Differentiation Status Of Human Embryonic Stem Cells Noninvasively Using Raman Microspectroscopy. Analytical Chemistry 2010, 82, 5020 - 5027.BibTex Konorov, S. O. ; H. Schulze, G. ; Caron, N. J. ; Piret, J. M. ; Blades, M. W. ; Turner, R. F. B. Raman Microspectroscopic Evidence That Dry-Fixing Preserves The Temporal Pattern Of Non-Specific Differentiation In Live Human Embryonic Stem Cells. Journal of Raman Spectroscopy 2010, 42, 576 - 579.BibTex 2009 Robb, D. B. ; Blades, M. W. An Electropneumatic-Heated Nebulizer For Enhancing Spray Ionization In Photospray Atmospheric Pressure Photoionization Sources For Liquid Chromatography/mass Spectrometry. Rapid Communications in Mass Spectrometry 2009, 23, 3394-3400.BibTex Smith, D. R. ; Robb, D. B. ; Blades, M. W. Comparison Of Dopants For Charge Exchange Ionization Of Nonpolar Polycyclic Aromatic Hydrocarbons With Reversed-Phase Lc-Appi-Ms. Journal of the American Society for Mass Spectrometry 2009, 20, 73-79.BibTex Konorov, S. O. ; H. Schulze, G. ; Addison, C. J. ; Haynes, C. A. ; Turner, R. F. B. ; Blades, M. W. Temperature-Dependent Excited State Absorption In Dna And Lna Oligomers Supports An Emerging Model Of Excited State Dynamics In Dna. The Open Spectroscopy Journal 2009, 3, 43 - 51.BibTex