Le, X. Chris - University of Alberta - Department of Medicine

个人简介

As a Distinguished University Professor, Dr. Le contributes to Department of Laboratory Medicine and Pathology (Faculty of Medicine and Dentistry), Department of Public Health Sciences (School of Public Health), and Department of Chemistry (Faculty of Science), University of Alberta. He is Director of the Analytical and Environmental Toxicology Division. Dr. Le served or serves as an Associate Editor for Analytical Chemistry, Associate Editor for Environmental Health Perspectives, and Editor-in-Chief for Journal of Environmental Sciences.. Dr. Le received his Ph.D. in Environmental/Analytical Chemistry from the University of British Columbia (1990-1993). He was subsequently awarded a prestigious Killam Postdoctoral Fellowship and carried out postdoctoral research in the Chemistry Department at the University of Alberta (1994). He was recruited to the Faculty of Medicine in 1995 and was promoted through the ranks to Professor in 2003. Dr. Le held an inaugural Canada Research Chair in Bio-Analytical Technology and Environmental Health (2000-2017). He was awarded an E.W.R. Steacie Fellowship (2000-2001) from the Natural Sciences and Engineering Research Council of Canada (NSERC). He also received from the Canadian Society for Chemistry a McBryde Medal (2002) and a Maxxam Award (2011) for "distinguished contribution to the field of analytical chemistry", as well as an Environment Research and Development Award (2011) "for distinguished contributions to research and/or development in the fields of environmental chemistry or environmental chemical engineering". He also received the University Cup (2018), Distinguished University Professorship (2011), Award for Excellence in Mentoring (2009) and a Martha Cook Piper Research Prize (2000), from the University of Alberta. Dr. Le is elected Fellow of the Chemical Institute of Canada (2001), Fellow of the Royal Society of Chemistry (UK) (2016), and Fellow of the Academy of Science, Royal Society of Canada (2010).

研究领域

Chemical speciation studies are essential to an understanding of toxicity and bioavailability of the element in the environment. Although total element concentration has been traditionally used to assess environmental impact and health risk of the element, it is now established that no meaningful interpretation can be made without speciation information. Each chemical form of an element has a different toxicity. For example, the toxicity of arsenic to animals varies from the more toxic arsenite, to the moderately toxic methylated arsenicals, to the essentially non-toxic arsenobetaine. We are interested in chemical speciation studies of metalloids (such as arsenic, antimony, and selenium) and heavy metals (such as mercury, lead, and cadmium) in the environment and biological systems, in view of their important impact on the environment and potential health risk.

近期论文

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Feng, W.; Peng, H.; Zhang, H.; Weinfeld, M.; Le, X.C.A sensitive technique unravels the kinetics of activation and trans-cleavage of CRISPR-Cas systems. Angewandte Chemie International Edition, March 25, 2024. DOI: 10.1002/anie.202404069 Tao, J.; Zhang, H.; Weinfeld, M.; Le, X.C.Detection of uracil-excising DNA glycosylases in cancer cell samples using a three-dimensional DNAzyme walker. ACS Measurement Science Au, May 8, 2024 DOI: 10.1021/acsmeasuresciau.4c00011 Zhao, Y.; Zhao, X.; Duan, L.; Hou, R.; Gu, Y.; Liu, Z.; Chen, J.; Wu, F.; Yang, L.; Le, X.C.; Wang, Q.; Yan, X.Reinvent aliphatic arsenicals as reversible covalent warheads toward targeted kinase inhibition and non-acute promyelocytic leukemia cancer treatment. Journal of Medicinal Chemistry, 2024, 67(7), 5458–5472. DOI: 10.1021/acs.jmedchem.3c02076 Hu, J.; Yan, X.; Le, X.C.Label-free detection of biomolecules using inductively coupled plasma mass spectrometry (ICP-MS). Analytical and Bioanalytical Chemistry, 2024, 416, 2625-2640. DOI: 10.1007/s00216-023-05106-7 Kumblathan, T.; Liu, Y.; Crisol, M.; Pang, X.; Hrudey, S.E.; Le, X.C.; Li, X.-F. Advances in wastewater analysis revealing the co-circulating viral trends of noroviruses and omicron subvariants. Science of the Total Environment, 2024, 920, 170887. DOI: 10.1016/j.scitotenv.2024.170887 Tao, J.; Zhang, H.; Weinfeld, M.; Le, X.C.Development of a DNAzyme walker for the detection of APE1 in living cancer cells. Analytical Chemistry, 2023, 95 (40), 14990–14997 DOI: 10.1021/acs.analchem.3c02574 Hoy, K.S.; Davydiuk, T.; Chen, X.; Lau, C.; Schofield, J.R.M.; Lu, X.; Graydon, J.A.; Mitchell, R.; Reichert, M.; Le, X.C.Arsenic speciation in freshwater fish: Challenges and research needs. Food Quality and Safety, 2023, 7, 1–25. DOI: 10.1093/fqsafe/fyad032 Davydiuk, T.; Tao, J.; Lu, X.; Le, X.C.Effects of dietary intake of arsenosugars and other organic arsenic species on studies of arsenic methylation efficiency in humans. Environment & Health, 2023, 1 (4), 236–248. DOI: 10.1021/envhealth.3c00090 Kumblathan, T.; Liu, Y.; Pang, L.; Hrudey, S.E.; Le, X.C.; Li, X.F.Quantification and differentiation of SARS-CoV-2 variants in wastewater for surveillance. Environment & Health, 2023, 1 (3), 203–213. DOI: 10.1021/envhealth.3c00089 Liu, Y.; Kumblathan, T.; Joyce, M.A.; Tyrrell, D.L.; Tipples, G.; Pang, X.; Li, X.-F.; Le, X. C.Multiplex assays enable simultaneous detection and identification of SARS-CoV-2 variants of concern in clinical and wastewater samples. ACS Measurement Science Au, 2023, 3 (4), 258–268. DOI: 10.1021/acsmeasuresciau.3c00005 Feng, W.; Zhang, H.; Le, X.C.Signal amplification by the trans-cleavage activity of CRISPR-Cas systems: Kinetics and performance. Analytical Chemistry, 2023, 95 (1), 206–217. DOI: 10.1021/acs.analchem.2c04555 Xiao, H.; Hu, J.; Huang, C.; Feng, W.; Liu, Y.; Kumblathan, T.; Tao, J.; Xu, J.; Le, X.C.; Zhang, H.CRISPR techniques and potential for the detection and discrimination of SARS-CoV-2 variants of concern. Trends in Analytical Chemistry, 2023, 161, 117000. DOI: 10.1016/j.trac.2023.117000 Liu, Y.; Kumblathan, T.; Tao, J.; Xu, J.; Feng, W.; Xiao, H.; Hu, J.; Huang, C.; Wu, Y.; Zhang, H.; Li, X.-F.; Le, X.C.Recent advances in RNA sample preparation techniques for the detection of SARS-CoV-2 in saliva and gargle. Trends in Analytical Chemistry, 2023, 165, 117107. DOI: 10.1016/j.trac.2023.117107 Lau, C.; Le, X.C.Cadmium, chromium, copper, iron, lead, mercury, nickel, and zinc in freshwater fish: Assessing trophic transfer using stable isotope ratios of d15N and d13C. Journal of Environmental Sciences, 2023, 128, 250–257. DOI: 10.1016/j.jes.2023.01.007 Kumblathan, T.; Liu, Y.; Qiu, Y.; Pang, L.; Hrudey, S.E.; Le, X.C.; Li, X.-F.An efficient method to enhance recovery and detection of SARS-CoV-2 RNA in wastewater. Journal of Environmental Sciences, 2023, 130, 139–148. DOI: 10.1016/j.jes.2022.10.006 Moreno-Bondi, M.C.; Le, X.C.; Field, J.A.; Richardson, S.D.; Li, X.-F.; Diamond, M.L.; Li, X.; Goring, P.D.From detection to remediation: Analytical science at the forefront of environmental research. Analytical Chemistry, 2022, 94 (43), 14811–14814. DOI: 10.1021/acs.analchem.2c04534 ACS Omega, 2022, 7 (43), 38105–38108. DOI: 10.1021/acsomega.2c06631 Liu, Y.; Kumblathan, T.; Feng, W.; Pang, B.; Tao, J.; Xu, J.; Xiao, H.; Joyce, M.A.; Tyrrell, D.L.; Zhang, H.; Li, X.-F.; Le, X.C.On-site viral inactivation and RNA preservation of gargle and saliva samples combined with direct analysis of SARS-CoV-2 RNA on magnetic beads. ACS Measurement Science Au, 2022, 2 (3), 224–232. DOI: 10.1021/acsmeasuresciau.1c00057 ACS Editors' Choice article Deng, W.; Xu, J.Y.; Peng, H.; Huang, C.Z.; Le, X.C.; Zhang, H.DNAzyme motor systems and logic gates facilitated by toehold exchange translators. Biosensors and Bioelectronics, 2022, 217, 114704. DOI: 10.1016/j.bios.2022.114704 Feng,W.; Xiao, H.; Yuan, A.; Deng, Z.; Wang, X.; Peng, H.; Le, X.C.Integrating CRISPR and isothermal amplification reactions in single-tubes for ultrasensitive detection of nucleic acids: the SARS-CoV-2 RNA example. Sci. Sin. Chim., 2022, 52, 1–14. DOI: 10.1360/SSC-2022-0076 Li, Q.; Yao, J.J.; Jiang, Y.N.; Guo, X.Y.; Ying, Y.; Wen, Y.; Liu, X.L.; Wu, Y.P.; Yang, H.F.; Le, X.C.One-step preparation of SnO2-AuNPs as nanocomposites on photoelectrodes to enhance photoelectrochemical detection of nitrite and superoxide" Journal of Electroanalytical Chemistry, 2022, 917, 116412. DOI: 10.1016/j.jelechem.2022.116412 MacDonald, A.M.; Gabos, S.; Braakman, S.; Cheperdak, L.; Lee, B.; Hrudey, S.E.; Le, X.C.; Li, X.-F.; Mandal, R.; Martin, J.W.; Schopflocher, D.; Lyon, M.E.; Cheung, P.-Y.; Ackah, F.; Graydon, J.A.; Reichert, M.; Lyon, A.W.; Jarrell, J.; Benadé, G.; Charlton, C.; Huang, D.; Bennett, M.J.; Kinniburgh, D.W. Maternal and Child Biomonitoring Strategies and Levels of Exposure in Western Canada During the Past Fifteen Years: The Alberta Biomonitoring Program 2005–2021. Cao, Y.; Zhang, H.; Le, X. C.Split locations and secondary structures of a DNAzyme critical to binding-assembled multicomponent nucleic acid enzymes for protein detection. Analytical Chemistry, 2021, 93 (47), 15712–15719. DOI: 10.1021/acs.analchem.1c03617 Feng, W.; Peng, H.; Xu, J.; Liu, Y.; Pabbaraju, K.; Tipples, G.; Joyce, M.; Saffran, H.; Tyrrell, D. L.; Babiuk, S.; Zhang, H.; Le, X. C.Integrating reverse transcription recombinase polymerase amplification with CRISPR technology for one-tube assay of RNA. Analytical Chemistry, 2021, 93 (37), 12808-12816. DOI: 10.1021/acs.analchem.1c03456 Feng, W.; Newbigging, A.M.; Tao, J.; Cao, Y.; Peng, H.; Le, C.; Wu, J.; Pang, B.; Li, J.; Tyrrell, D. L.; Zhang, H.; Le, X. C.CRISPR technology incorporating amplification strategies: Molecular assays for nucleic acids, proteins, and small molecules. Chemical Science, 2021, 12 (13), 4683-4698. DOI: 10.1039/D0SC06973F. Wu, J.; Peng, H.; Lu, X.; Lai, M.; Zhang, H.; Le, X. C.Binding-mediated formation of ribonucleoprotein corona for efficient delivery and control of CRISPR-Cas9. Angewandte Chemie International Edition, 2021, 60 (20), 11104–11109. DOI: 10.1002/anie.202014162 Le, C.; Liu, Y.; López-Orozco, J.; Joyce, M.; Le, X. C.; Tyrrell, D. L.A CRISPR technique incorporated with single-cell RNA sequencing for studying hepatitis B infection. Analytical Chemistry, 2021, 93 (31), 10756–10761. DOI: 10.1021/acs.analchem.1c02227 Le, X.C. Analytical advances in detecting SARS-CoV 2 and further research needs for COVID-19 testing. Analytical Chemistry, 2021, 93 (24), 8379–8380. DOI: 10.1021/acs.analchem.1c02327 Liu, Q.Q.; Huang, C.Z.; Li, W.H.; Fang, Z.Z.; Le, X.C.Discovery and identification of arsenolipids using a precursor-finder strategy and data-independent mass spectrometry. Environmental Science & Technology, 2021, 55 (6), 3836–3844. DOI: 10.1021/acs.est.0c07175 Cao, Y.; Wu, J.; Pang, B.; Zhang, H.; Le, X.C.CRISPR/Cas12a-mediated gold nanoparticle aggregation for colorimetric detection of SARS-CoV-2. Chemical Communications, 2021, 57 (56), 6871-6874. DOI: 10.1039/d1cc02546e Tang, Y.; Gao, L.; Feng, W.; Guo, C.; Yang, Q.; Li, F.*; Le, X.C.The CRISPR-Cas toolbox for analytial and diagnostic assay development. Chemical Society Reviews, 2021, 50 (21), 11844-11869. DOI: 10.1039/d1cs00098e Zhou, J.R.; Kaur, G.; Ma, Y.; Arutyunov, D.; Lu, X.; Le, X.C.; Leslie, E.M.Biliary excretion of arsenic by numan HepaRG cells is stimulated by selenide and mediated by the multidrug resistance protein 2 (MRP2/ABCC2). Biochemical Pharmacology, 2021, 193, 114799. DOI: 10.1016/j.bcp.2021.114799 Fu, Z.; Rais, Y.; Tarek A. Bismar, T.A.; Hyndman, M.E.; Le, X.C.; Drabovich, A.P.Mapping isoform abundance and interactome of the endogenous TMPRSS2-ERG fusion protein by orthogonal immunoprecipitation-mass spectrometry assays. Molecular & Cellular Proteomics, 2021, 20, 100075. DOI: 10.1016/j.mcpro.2021.100075 Qu, X.F.; Deng, X.Y.; Pang, Y.C.; Wang, W.L.; Feng, Y.W.; Xu, Z.H.; Zhang, Y.; Le, X.C.Recent advances in structural improvements and chemical modifications of lateral flow assay devices. Scientia Sinica Chimica, 2021, 51 (7), 905–918 DOI: 10.1360/SSC-2021-0025 Li, Z.; Chen, B.W.; Li, Y.B.; Le, X.C.Reduction of mercury emissions from anthropogenic sources including coal combustion. Journal of Environmental Sciences, 2021, 100, 363–268. DOI: 10.1016/j.jes.2020.11.002 Pang, B; Zhang, H.; Tyrrell, D.L.; Le, X.C.Isothermal amplification and ambient visualization in a single tube for the detection of SARS-CoV-2 using loop-mediated amplification and CRISPR technology. United States patent US 7/519,670 Le, X.C.; Zhang, H.; Peng, H.; Li, X.-F.A microRNA initiated DNAzyme motor operating in living cells. United States patent 10,934,162 B2. Pang, B.; Xu, J.; Liu, Y.; Peng, H.; Feng, W.; Cao, Y.; Wu, J.; Xiao, H.; Pabbaraju, K.; Tipples, G.; Joyce, M.A.; Saffran, H.A.; Tyrrell, D. L.; Zhang, H.; Le, X. C.Isothermal amplification and ambient visualization in a single tube for the detection of SARS-CoV-2 using loop-mediated amplification and CRISPR technology. Analytical Chemistry, 2020, 92, 16204–16212. DOI: 10.1021/acs.analchem.0c04047 Feng, W.; Newbigging, A.M.; Le, C.; Pang, B.; Peng, H.; Cao, Y.; Wu, J.; Abbas, G.; Song, J.; Wang, D.-B.; Cui, M.; Tao, J.; Tyrrell, D. L.; Zhang, X.-E.; Zhang, H.; Le, X. C.Molecular diagnosis of COVID-19: Challenges and research needs. Analytical Chemistry, 2020, 92 (15), 10196-10209. DOI: 10.1021/acs.analchem.0c02060 Liu, Y.; Le, C.; Tyrrell, D.L.; Le, X.C.; Li, X.-F.Aptamer binding assay for the E antigen of hepatitis B using modified aptamers with G-quadruplex structures. Analytical Chemistry, 2020, 92 (9), 6495-6501. DOI: 10.1021/acs.analchem.9b05740 Peng, H.; Le, C.; Wu, J.; Li, X.-F.; Zhang, H.; Le, X.C.A genome editing nanomachine constructed with a Clustered Regularly Interspaced Short Palindromic Repeats system and activated by near-infrared illumination. ACS Nano, 2020, 14 (3), 2817-2826. DOI: 10.1021/acsnano.9b05276 Peng, H.; Newbigging, A.M.; Reid, M.S.; Uppal, J.S.; Xu, J.Y.; Zhang, H.; Le, X.C.Signal amplification in living cells: A review of microRNA detection and imaging. Analytical Chemistry, 92 (1), 292-308. DOI: 10.1021/acs.analchem.9b04752 Zhao, Q.; Tao, J.; Feng, W.; Uppal, J.S.; Peng, H.; Le, X.C.Aptamer binding assays and molecular interaction studies using fluorescence anisotropy - A review. Analytica Chimica Acta , 2020, 1125, 267-278. DOI: 10.1016/j.aca.2020.05.061 Liu, Q.; Huang, C.Z.; Le, X.C.Arsenic species in electronic cigarettes: Determination and potential health risk. Journal of Environmental Sciences, 2020, 91, 168-176. DOI: 10.1016/j.jes.2020.01.023 Reid, M.S.; Hoy, K.S.; Uppal, J.S.; Schofield, J.R.M.; Lin, Y.; Lu, X.; Peng, H.; Le. X.C.Arsenic speciation analysis: A review with an emphasis on chromatographic separations. Trends in Analytical Chemistry, 2020, 123, 115770. DOI: 10.1016/j.trac.2019.115770 Huang K., Gao, F.; Le, X.C.; Zhao, F.-J.N-Hydroxyarylamine O-acetyltransferases 1 ENhoAs catalyze acetylation of 3-amino-4-hydroxyphenylarsonic acid in the 4-hydroxy-3-nitrobenzenearsonic acid (Roxarsone) transformation pathway of Enterobacter sp. CZ-1. Applied and Environmental Microbiology, 2020, 86 (2), e02050-19. DOI: 10.1128/AEM.02050-19 Davydiuk, T.; Chen, X.J.; Huang, L.J.; Shuai, Q.; Le, X.C.Removal of inorganic arsenic from water using metal organic frameworks. Journal of Environmental Sciences, 2020, 97, 162-168. DOI: 10.1016/j.jes.2020.08.012 Xie, J.J.; Schofield, J.R.M.; Liao, L.J.; Peng, H.Y.; Uppal, J.S.; Zheng, Q.; Le, X.C.Simultaneous removal of arsenic and antimony from mining wastewater. Journal of Environmental Sciences, 2020, 93, 117-119. DOI: 10.1016/j.jes.2020.04.003 Deng, W.C.; Schofield, J.R.M.; Le, X.C.; Li, X.-F.Electronic cigarettes and toxic substances, including arsenic species. Journal of Environmental Sciences, 2020, 92, 278-283. DOI: 10.1016/j.jes.2020.03.004 Chen, J.; Moe, B.; Zhu, L.Z.; Le, X.C.“Waste”-ing away: Presence of Cu ions influences microbial degradation kinetics and metabolite formation of the prevalent brominated flame retardant BDE-47. Journal of Environmental Sciences, 2020, 87, 421-424. DOI: 10.1016/j.jes.2019.10.002 Le, X.C.; Zhang, H.; Newbigging, A.N.Beacon-mediated Exponential Amplification Reaction (BEAR) Using a Single Enzyme and Primer. United States patent application, US 16/883,734. Peng, H.; Zhang, H.; Li, X.-F.; Le, X.C.Near-Infrared photothermally activated CRISPR/Cas9 genome editing machine. United States provisional patent application. United States patent application, US 62/899,927.