Wu, Huiyuan - 天津大学 - 药物科学与技术学院

个人简介

Education Experience： 2004-2008 Ph. D. Biochemistry Martin-Luther University, Halle-Wittenberg,Germany 1995-1998 M.S. Microbial Pharmacy Shenyang Pharmaceutical University, China 1991-1995 Bachelor Microbial Pharmaceutics Shenyang Pharmaceutical University, China Professional Experience： 2016- Associate Professor Tianjin University 2009-2016 Postdoctoral Fellow St. Jude Children's Research Hospital, USA 2008-2009 Postdoctoral Fellow Martin-Luther University, Halle-Wittenberg, Germany 2002-2003 UNESCO Course Participant Osaka University & Kyoto University, Japan 1998-2002 Lecture East China University of Science and Technology Honors & Awards： [1]Prize for Excellent Patent by Shanghai Bureau for Invention and Patent: 2002 [2]Participant of the 30th UNESCO Course in Microbiology: 2002-2003

研究领域

The research interest of Wu's Lab is to elucidate novel molecular mechanisms that underlie pathological neuronal death and neuronal regeneration and to provide the new therapeutic strategy to treat neurodegenerative disorders. Our primary interests focus on the novel mechanisms common in neuronal degeneration and regeneration contributed by a unique protein post-translational modification, polyglutamylation. Polyglutamylation is a dynamic process in which glutamate residues are added to the γ-carboxyl of a glutamate in the primary amino acid chain of substrate proteins by tubulin tyrosine ligase-like enzymes (TTLLs) and removed by the Cytosolic Carboxypeptidase (CCP) family. The prototype of CCP family, Nna1 (a.k.a CCP1) was discovered in an axon regeneration model and its dysfunction was later found underlying the phenotypes of Purkinje cell degeneration (pcd) mutant mice, in which certain types of neurons undergo progressive degeneration. Interestingly, a growing body of evidence shows that not only deficits of the catabolic process but also the anabolic process of polyglutamylation can cause varieties of neurodegenerative disorders, as well as other anormlies such as male infertility and defective hemostasis. Our lab uses genetically-manipulated mouse model combined with an array of methodologies of biochemistry, cell biology, and structural biology to reveal the genetic pathway and molecular insights that underlie the disorders caused by imbalanced polyglutamylation homeostasis. We are also interested in drug screen using neurodegeneration mouse models.

近期论文

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CCP5 and CCP6 retain CP110 and negatively regulate ciliogenesis Yujuan Wang, Yuan Zhang, Xinyu Guo, Yiqiang Zheng, Xinjie Zhang, Shanshan Feng & Hui-Yuan Wu BMC Biology volume 21, Article number: 124 (2023) Facile purification of active recombinant mouse cytosolic carboxypeptidase 6 from Escherichia coli Guo Xinyu,Wang Ruixue,Ma Ruifang,Fan Xiaona,Gao Yan,Zhang Xiangyang,Yuchi Zhiguang,Wu Hui-Yuan Page:106112 ISSN:1046-5928 2022 Container-title:Protein Expression and Purification A lysosome-targeted probe for the real-time detection of hypobromous acid in living human cancer cells Wang Yali,Zhang Yuan,Yang Lijun,Wu Huiyuan,Finney Nathaniel Page:2484-2489 ISSN:0003-2654 2021 Container-title:The Analyst Effects of lysine 2-hydroxyisobutyrylation on bacterial FabI activity and resistance to triclosan Biochimie 2021-03 | Journal article DOI: 10.1016/j.biochi.2021.01.011 CONTRIBUTORS: Yiqiang Zheng; Hanyang Dong; Xue Bai; Hui Cui; Mulin Jun Li; Hui-Yuan Wu; Kai Zhang Identification of 2-PMPA as a novel inhibitor of cytosolic carboxypeptidases Wang Ruixue,Lin Lianyun,Zheng Yiqiang,Cao Peng,Yuchi Zhiguang,Wu Hui-Yuan Page:1393-1399 ISSN:0006-291X 2020 Container-title:Biochemical and Biophysical Research Communications Expression of recombinant mouse cytosolic carboxypeptidase 6 in Escherichia coli Wang Ruixue,Wu Hui-Yuan Page:04050 ISSN:2267-1242 Container-title:E3S Web of Conferences https://doi.org/10.1051/e3sconf/202018504050 Identification of dual histone modification-binding protein interaction by combining mass spectrometry and isothermal titration calorimetric analysis Journal of Advanced Research 2020-03 | Journal article DOI: 10.1016/j.jare.2019.11.003 CONTRIBUTORS: Pu Chen; Zhenchang Guo; Cong Chen; Shanshan Tian; Xue Bai; Guijin Zhai; Zhenyi Ma; Huiyuan Wu; Kai Zhang