个人简介
Education Experience:
2007-2013 Postdoctoral Synthetic Biology ETH Zurich
2000-2002 Ph. D. Physics University of Trento (Italy)
1994-1999 M.S. Physics University of Eastern Piedmont (Italy)
Professional Experience:
2003-2007 Analyst programmer CILEA (Italy)
2013-2018 Associate Professor in Synthetic Biology Harbin Institute of Technology
研究领域
Synthetic Biology is referred to as Life Engineering since it aims at modifying cells with the insertion of DNA circuits that carry out new, specific tasks. Possible applications are diagnostics, cure of disease, biofuel production, and environmental care. Research in Synthetic Biology is both theoretical and applied. Circuits are first designed on the computer. They are associated with mathematical models such that simulations can drive their wet-lab implementation.
On the computational side I am working on the development of a stand-alone piece of software for the design of biosensors in living cells. Biosensors detect one or more chemicals (inputs) in the cellular environment and, as a response, trigger the production of a clear output signal (e.g. fluorescence) or the activation of a pathway that establishes an interaction between the cells and the sensed chemicals. For instance, if the input is a pollutant, a properly engineered biosensor could lead to the degradation of this harmful substance.
Biosensors designed on the computer are implemented in my lab into the yeast S. cerevisiae. Yeast is the simplest eukaryotic organism and, as such, a perfect candidate to develop theoretical models and build biosensors that might be used later into more complex hosts such as mammalian cells.
Overall, building genetic biosensors demands a proper characterization of its basic components (promoters, mRNAs, and terminators) and the optimization of mechanisms for the regulation, in yeast, of transcription and translation processes (e.g. CRISPR-Cas9, TAL effectors, riboswitches, PUF proteins). These are two important research directions in my Synthetic Biology lab.
近期论文
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Engineered yeasts and lignocellulosic biomaterials: shaping a new dimension for biorefinery and global bioeconomy
Michael Dare Asemoloye,Tunde Sheriffdeen Bello,Peter Olusakin Oladoye,Muideen Remilekun Gbadamosi,Segun Oladiran Babarinde,Gboyega Ebenezer Adebami,Olumayowa Mary Olowe,Marta Elisabetta Eleonora Temporiti,Wolfgang Wanek &Mario Andrea Marchisio
Article: 2269328 | Received 24 Jun 2023, Accepted 03 Oct 2023, Published online: 18 Oct 2023
https://doi.org/10.1080/21655979.2023.2269328
Hybrid Boolean gates show that Cas12c controls transcription activation effectively in the yeast S. cerevisiae
Liu Yifan,Ge Huanhuan,Marchisio Mario Andrea
ISSN:2296-4185 2023
Container-title:Frontiers in Bioengineering and Biotechnology
CRISPR-associated type V proteins as a tool for controlling mRNA stability in S. cerevisiae synthetic gene circuits
Nucleic Acids Research
2023-02-22 | Journal article
DOI: 10.1093/nar/gkac1270
CONTRIBUTORS: Lifang Yu; Mario Andrea Marchisio
Synthetic metabolic transducers in Saccharomyces cerevisiae as sensors for aromatic permeant acids and bioreporters of hydrocarbon metabolism
Biosensors and Bioelectronics
2023-01 | Journal article
DOI: 10.1016/j.bios.2022.114897
CONTRIBUTORS: Michael Dare Asemoloye; Mario Andrea Marchisio
Logic Circuits Based on 2A Peptide Sequences in the Yeast Saccharomyces cerevisiae
ACS Synthetic Biology
2023-01-20 | Journal article
DOI: 10.1021/acssynbio.2c00506
CONTRIBUTORS: Xuekun Wang; Ximing Tian; Mario Andrea Marchisio
Engineering a two-gene system to operate as a highly sensitive biosensor or a sharp switch upon induction with β-estradiol
Scientific Reports
2022-12-16 | Journal article
DOI: 10.1038/s41598-022-26195-x
CONTRIBUTORS: Tian Zhou; Zhiying Liang; Mario Andrea Marchisio
Feedforward Loops: Evolutionary Conserved Network Motifs Redesigned for Synthetic Biology Applications
Applied Sciences
2022-08-19 | Journal article
DOI: 10.3390/app12168292
CONTRIBUTORS: Tsigereda Weldemichael; Michael Dare Asemoloye; Mario Andrea Marchisio
Interaction of Bare dSpCas9, Scaffold gRNA, and Type II Anti-CRISPR Proteins Highly Favors the Control of Gene Expression in the Yeast S. cerevisiae
ACS Synthetic Biology
2022-01-21 | Journal article
DOI: 10.1021/acssynbio.1c00352
CONTRIBUTORS: Yadan Zhang; Mario Andrea Marchisio
Synthetic polycistronic sequences in eukaryotes
Synthetic and Systems Biotechnology
2021-12 | Journal article
DOI: 10.1016/j.synbio.2021.09.003
CONTRIBUTORS: Xuekun Wang; Mario Andrea Marchisio
Type II anti-CRISPR proteins as a new tool for synthetic biology
RNA Biology
2021-08-03 | Journal article
DOI: 10.1080/15476286.2020.1827803
CONTRIBUTORS: Yadan Zhang; Mario Andrea Marchisio
Saccharomyces cerevisiae Promoter Engineering before and during the Synthetic Biology Era
Biology
2021-06-06 | Journal article
DOI: 10.3390/biology10060504
CONTRIBUTORS: Xiaofan Feng; Mario Marchisio
Novel S. cerevisiae Hybrid Synthetic Promoters Based on Foreign Core Promoter Sequences
International Journal of Molecular Sciences
2021-05-27 | Journal article
DOI: 10.3390/ijms22115704
CONTRIBUTORS: Xiaofan Feng; Mario Andrea Marchisio
Futile cycling by human microsomal cytochrome P450 enzymes within intact fission yeast cells
Archives of Biochemistry and Biophysics
2021-04 | Journal article
DOI: 10.1016/j.abb.2021.108791
CONTRIBUTORS: Dawit M. Weldemichael; Kun Zhou; Shi-jia Su; Lin Zhao; Mario Andrea Marchisio; Matthias Bureik
Saccharomyces cerevisiae Synthetic Transcriptional Networks Harnessing dCas12a and Type V-A anti-CRISPR Proteins
ACS Synthetic Biology
2021-04-16 | Journal article
DOI: 10.1021/acssynbio.1c00006
CONTRIBUTORS: Lifang Yu; Mario Andrea Marchisio
Aptamers, Riboswitches, and Ribozymes in S. cerevisiae Synthetic Biology
Life
2021-03-17 | Journal article
DOI: 10.3390/life11030248
CONTRIBUTORS: Huanhuan Ge; Mario Andrea Marchisio
Hydrocarbon Degradation and Enzyme Activities of Aspergillus oryzae and Mucor irregularis Isolated from Nigerian Crude Oil-Polluted Sites
Microorganisms
2020-11-30 | Journal article
DOI: 10.3390/microorganisms8121912
CONTRIBUTORS: Michael Dare Asemoloye; Solveig Tosi; Chiara Daccò; Xiao Wang; Shihan Xu; Mario Andrea Marchisio; Wenyuan Gao; Segun Gbolagade Jonathan; Lorenzo Pecoraro