Marchisio, MarioAndrea - 天津大学 - 药物科学与技术学院

个人简介

Education Experience： 2007-2013 Postdoctoral Synthetic Biology ETH Zurich 2000-2002 Ph. D. Physics University of Trento (Italy) 1994-1999 M.S. Physics University of Eastern Piedmont (Italy) Professional Experience： 2003-2007 Analyst programmer CILEA (Italy) 2013-2018 Associate Professor in Synthetic Biology Harbin Institute of Technology

研究领域

Synthetic Biology is referred to as Life Engineering since it aims at modifying cells with the insertion of DNA circuits that carry out new, specific tasks. Possible applications are diagnostics, cure of disease, biofuel production, and environmental care. Research in Synthetic Biology is both theoretical and applied. Circuits are first designed on the computer. They are associated with mathematical models such that simulations can drive their wet-lab implementation. On the computational side I am working on the development of a stand-alone piece of software for the design of biosensors in living cells. Biosensors detect one or more chemicals (inputs) in the cellular environment and, as a response, trigger the production of a clear output signal (e.g. fluorescence) or the activation of a pathway that establishes an interaction between the cells and the sensed chemicals. For instance, if the input is a pollutant, a properly engineered biosensor could lead to the degradation of this harmful substance. Biosensors designed on the computer are implemented in my lab into the yeast S. cerevisiae. Yeast is the simplest eukaryotic organism and, as such, a perfect candidate to develop theoretical models and build biosensors that might be used later into more complex hosts such as mammalian cells. Overall, building genetic biosensors demands a proper characterization of its basic components (promoters, mRNAs, and terminators) and the optimization of mechanisms for the regulation, in yeast, of transcription and translation processes (e.g. CRISPR-Cas9, TAL effectors, riboswitches, PUF proteins). These are two important research directions in my Synthetic Biology lab.

近期论文

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Engineered yeasts and lignocellulosic biomaterials: shaping a new dimension for biorefinery and global bioeconomy Michael Dare Asemoloye,Tunde Sheriffdeen Bello,Peter Olusakin Oladoye,Muideen Remilekun Gbadamosi,Segun Oladiran Babarinde,Gboyega Ebenezer Adebami,Olumayowa Mary Olowe,Marta Elisabetta Eleonora Temporiti,Wolfgang Wanek &Mario Andrea Marchisio Article: 2269328 | Received 24 Jun 2023, Accepted 03 Oct 2023, Published online: 18 Oct 2023 https://doi.org/10.1080/21655979.2023.2269328 Hybrid Boolean gates show that Cas12c controls transcription activation effectively in the yeast S. cerevisiae Liu Yifan,Ge Huanhuan,Marchisio Mario Andrea ISSN:2296-4185 2023 Container-title:Frontiers in Bioengineering and Biotechnology CRISPR-associated type V proteins as a tool for controlling mRNA stability in S. cerevisiae synthetic gene circuits Nucleic Acids Research 2023-02-22 | Journal article DOI: 10.1093/nar/gkac1270 CONTRIBUTORS: Lifang Yu; Mario Andrea Marchisio Synthetic metabolic transducers in Saccharomyces cerevisiae as sensors for aromatic permeant acids and bioreporters of hydrocarbon metabolism Biosensors and Bioelectronics 2023-01 | Journal article DOI: 10.1016/j.bios.2022.114897 CONTRIBUTORS: Michael Dare Asemoloye; Mario Andrea Marchisio Logic Circuits Based on 2A Peptide Sequences in the Yeast Saccharomyces cerevisiae ACS Synthetic Biology 2023-01-20 | Journal article DOI: 10.1021/acssynbio.2c00506 CONTRIBUTORS: Xuekun Wang; Ximing Tian; Mario Andrea Marchisio Engineering a two-gene system to operate as a highly sensitive biosensor or a sharp switch upon induction with β-estradiol Scientific Reports 2022-12-16 | Journal article DOI: 10.1038/s41598-022-26195-x CONTRIBUTORS: Tian Zhou; Zhiying Liang; Mario Andrea Marchisio Feedforward Loops: Evolutionary Conserved Network Motifs Redesigned for Synthetic Biology Applications Applied Sciences 2022-08-19 | Journal article DOI: 10.3390/app12168292 CONTRIBUTORS: Tsigereda Weldemichael; Michael Dare Asemoloye; Mario Andrea Marchisio Interaction of Bare dSpCas9, Scaffold gRNA, and Type II Anti-CRISPR Proteins Highly Favors the Control of Gene Expression in the Yeast S. cerevisiae ACS Synthetic Biology 2022-01-21 | Journal article DOI: 10.1021/acssynbio.1c00352 CONTRIBUTORS: Yadan Zhang; Mario Andrea Marchisio Synthetic polycistronic sequences in eukaryotes Synthetic and Systems Biotechnology 2021-12 | Journal article DOI: 10.1016/j.synbio.2021.09.003 CONTRIBUTORS: Xuekun Wang; Mario Andrea Marchisio Type II anti-CRISPR proteins as a new tool for synthetic biology RNA Biology 2021-08-03 | Journal article DOI: 10.1080/15476286.2020.1827803 CONTRIBUTORS: Yadan Zhang; Mario Andrea Marchisio Saccharomyces cerevisiae Promoter Engineering before and during the Synthetic Biology Era Biology 2021-06-06 | Journal article DOI: 10.3390/biology10060504 CONTRIBUTORS: Xiaofan Feng; Mario Marchisio Novel S. cerevisiae Hybrid Synthetic Promoters Based on Foreign Core Promoter Sequences International Journal of Molecular Sciences 2021-05-27 | Journal article DOI: 10.3390/ijms22115704 CONTRIBUTORS: Xiaofan Feng; Mario Andrea Marchisio Futile cycling by human microsomal cytochrome P450 enzymes within intact fission yeast cells Archives of Biochemistry and Biophysics 2021-04 | Journal article DOI: 10.1016/j.abb.2021.108791 CONTRIBUTORS: Dawit M. Weldemichael; Kun Zhou; Shi-jia Su; Lin Zhao; Mario Andrea Marchisio; Matthias Bureik Saccharomyces cerevisiae Synthetic Transcriptional Networks Harnessing dCas12a and Type V-A anti-CRISPR Proteins ACS Synthetic Biology 2021-04-16 | Journal article DOI: 10.1021/acssynbio.1c00006 CONTRIBUTORS: Lifang Yu; Mario Andrea Marchisio Aptamers, Riboswitches, and Ribozymes in S. cerevisiae Synthetic Biology Life 2021-03-17 | Journal article DOI: 10.3390/life11030248 CONTRIBUTORS: Huanhuan Ge; Mario Andrea Marchisio Hydrocarbon Degradation and Enzyme Activities of Aspergillus oryzae and Mucor irregularis Isolated from Nigerian Crude Oil-Polluted Sites Microorganisms 2020-11-30 | Journal article DOI: 10.3390/microorganisms8121912 CONTRIBUTORS: Michael Dare Asemoloye; Solveig Tosi; Chiara Daccò; Xiao Wang; Shihan Xu; Mario Andrea Marchisio; Wenyuan Gao; Segun Gbolagade Jonathan; Lorenzo Pecoraro