个人简介

B. S. 2001, Nanjing University, Ph. D., 2007, Rice University, (Advisor: Naomi J. Halas), Postdoctoral fellow, 2007-2010, University of Texas at Austin, (Advisor: Paul F. Barbara) NSF CAREER Award, 2013; USC Breakthrough Star Award, 2014;

研究领域

Physical

Research Areas: Nanoscience; Surface-enhanced spectroscopy; Biophysics; Bioanalytical chemistry. The central theme of our research is to use physical chemistry approaches, specifically spectroscopy and electrochemistry, to tackle challenging problems in materials and biological sciences. Nanostructures with Geometrically Tunable Optical Properties. Nanophotonic materials have emerged as an important class of subwavelength optical components that interact with light in unique ways on the nanometer length-scale. Our interests in nanophotonic materials broadly span several important aspects in this field, including but not limited to nanostructure fabrication, nanoscale self-assembly, structure-property relationship, and applications of these materials. We have been working on several nanostructures with geometrically tunable optical properties, such as semiconductor nanoshells, metal-semiconductor core-shell hetero- nanostructures, and complex metallic nanoparticles. We are particularly interested in (i) development of new approaches for the controllable fabrication of optically active nanostructures; (ii) utilization of optically active nanostructures as building blocks to assemble mesoscopic structures over multiple length scales; (iii) detailed characterization of the optical properties of these nanomaterials both at the ensemble and single-nanoparticle levels; (iv) development of quantitative understanding on the structure-property relationship of these nanostructures through combined experimental and theoretical efforts; and (v) applications of these nanophotonic materials in surface-enhanced spectroscopies for ultrasensitive molecular sensing. Multifunctional Nanoprobes for Electrochemical Cytosensing. The past two decades have witnessed significant progress on the development of robust analytical tools with high sensitivity, selectivity, and reproducibility toward early diagnosis of cancer. Electrochemical cytosensing has emerged as an extremely attractive method that can be readily implemented into quantitative bioassays for high-throughput clinical applications. Utilization of rationally designed multifunctional nanoprobes and specifically tailored nano-biointerfaces for electrochemical cytosensing provides unique opportunities to optimize the interfacial electron transfer and cell recognition processes, allowing for the integration of large signal amplification, enhanced detection specificity, and expanded multiplex sensing capabilities on one cytosensor. Our group has been working on the design and fabrication of multifunctional hybrid nanoprobes for selective and ultrasensitive electrochemical detection of a variety of cancer cells, such as leukemia cells and circulating tumor cells. Our electrochemical approaches also allow for the quantification of the expression levels of important biomarkers on the cancer cell surfaces. Single-Molecule Biophysics. Our group has been working on the development of detailed, molecular-level understanding of important nucleic acid (NA) structural remodeling processes chaperoned by retroviral nucleocapsid (NC) proteins. In spite of their structural simplicity, retroviral NC proteins exhibit a diverse set of biological functions that are crucial to the retroviral life-cycles. The role of NC proteins as NA chaperones is perhaps their most important function ever known so far. NC proteins promote several NA structural remodeling processes that are crucial to the retroviral life-cycles, such as the obligatory strand transfers in the reverse transcription, the maintenance and integration of proviral DNA, and the genomic RNA protection and packaging. NC-chaperoned NA structural remodeling typically involves various intermediates along multiple reaction pathways and is tightly associated with heterogeneous conformational dynamics over multiple time-scales that cannot be synchronized and resolved by ensemble measurements. Single-molecule spectroscopy provides an extremely powerful way to characterize such complex biomolecular processes without the ensemble averaging effects. Our group uses time-resolved single-molecule spectroscopy as an analytical tool to study the kinetics and mechanism of NC-chaperoned NA annealing that occurs during the reverse transcription and to resolve the conformational dynamics associated with NC-induced local bending of proviral DNA. These single-molecule measurements allow us to gain molecular-level insights on the complicated, dynamic NC-NA interactions that underpin NCs’ NA chaperone functions.

近期论文

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Jing, H.; Zhang, Q. F.; Large, N.; Yu, C. M.; Blom, D. A.; Nordlander, P.; Wang, H.*, Tunable Plasmonic Nanoparticles with Catalytically Active High Index Facets. Nano Lett. 2014, 14, 3674−3682. Zheng, T. T.; Zhang, Q. F.; Feng, S.; Zhu, J. J.*; Wang, Q.; Wang, H.*, Robust Nonenzymatic Hybrid Nanoelectrocatalysts for Signal Amplification toward Ultrasensitive Electrochemical Cytosensing. J. Am. Chem. Soc. 2014, 136 (6), 2288-2291. Zhang, Q. F.; Large, N.; Nordlander, P.; Wang, H.*, Porous Au Nanoparticles with Tunable Plasmon Resonances and Intense Field Enhancements for Single-Particle SERS. J. Phys. Chem. Lett. 2014, 5 (2), 370-374. Zheng, T.; Zhang, R.; Zhang, Q.; Tan, T.; Zhang, K.; Zhu, J. J.*; Wang, H.*, ""Ultrasensitive Dual-Channel Detection of Matrix Metalloproteinase-2 in Human Serum Using Gold-Quantum Dot Core-Satellite Nanoprobes"", Chemical Communications 2013, 49, 7881-7883. Zheng, T.; Tan, T.; Zhang, Q.; Fu, J. J.; Wu, J. J.; Zhang, K.; Zhu, J. J.*; Wang, H.*, ""Multiplex Acute Leukemia Cytosensing Using Multifunctional Hybrid Electrochemical Nanoprobes at Hierarchically Nanoarchitectured Electrode Interface"", Nanoscale 2013, 5 10360-10368. Zheng, T.; Fu, J. J.; Hu, L.; Qiu, F.; Hu, M.; Zhu, J. J.*; Hua, Z. C.*; Wang, H.*, ""Nanoarchitectured Electrochemical Cytosensors for Selective Detection of Leukemia Cells and Quantitative Evaluation of Death Receptor Expression on Cell Surfaces"", Analytical Chemistry 2013, 85, (11) 5609-5616. Wang, H.*; Musier-Forsyth, K.; Falk, C.; Barbara, P. F., “Single-Molecule Spectroscopic Study of Dynamic Nanoscale DNA Bending Behavior of HIV-1 Nucleocapsid Protein”, Journal of Physical Chemistry B 2013, 117, (16) 4183-4196. Zhang, L.; Jing, H.; Boisvert, G.; He, J. Z.; Wang, H.*, “Geometry Control and Optical Tunability of Metal-Cuprous Oxide Core-Shell Nanoparticles”, ACS Nano 2012, 6, (4)3514–3527. Qiu, C.; Zhang, L.; Wang, H.*; Jiang, C. Y.*, ""Surface-Enhanced Raman Scattering on Hierarchical Porous Cuprous Oxide Nanostructures in Nanoshell and Thin-Film Geometries"", Journal of Physical Chemistry Letters 2012, 3,(5) 651-657. Zhang, L.; Wang, H.*, “Interior Structural Tailoring of Cu2O Shell-In-Shell Nanostructures through Multi-Step Ostwald Ripening”, Journal of Physical Chemistry C 2011, 115, (38) 18479-18485. Zhang, L.; Blom, D. A.; Wang, H.*, “Au-Cu2O Core-Shell Nanoparticles: a Hybrid Metal-Semiconductor Heteronanostructure with Geometrically Tunable Optical Properties”, Chemistry of Materials 2011, 23 (20), 4587-4598. Zhang, L.; Wang, H.*, “Cuprous Oxide Nanoshells with Geometrically Tunable Optical Properties”, ACS Nano 2011, 5 (4), 3257-3267