SIMS, PAUL - University of Oklahoma - Department of Chemistry and Biochemistry

个人简介

BA, 1994, Adams State College PhD, 2005, University of Wisconsin-Madison

研究领域

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I am interested in the structure and function relationships of enzymes. Currently, we are studying the enzymes hydroxypyruvate isomerase and tartronate semialdehyde reductase (TSAR). We are working with Dr. Leonard Thomas of the OU Macromolecular X-ray Crystallography Laboratory to determine the structures of these enzymes. In addition, we also perform kinetic analyses, which help us understand the order of addition of substrates and the order of release of products (i.e., the kinetic mechanism). The structural studies complement the results of the kinetic analyses by providing (potentially) the identities of important active site residues. We test hypotheses about the roles of active-site residues by using site-directed mutagenesis to change these residues. We then purify the site-specific variants and test their catalytic activity. Collectively, these studies help us to determine the chemical mechanism, which is a description of the active-site residues that participate in the sequence of bond breaking and bond formation. This process provides us with a reasonably complete understanding of the catalytic functioning of these enzymes. Other research focuses on developing instructional and laboratory activities for undergraduate biochemistry courses. For example, we developed an inexpensive and relatively green method of purifying genomic DNA for use in large undergraduate laboratories, and we developed a computer activity in which spreadsheets are used to estimate the isoelectric point of proteins.