Geyer, Pamela - University of Iowa - Department of Biochemistry

个人简介

Education BS, McGill University PhD, Ohio State University Post Doctoral Fellow, Dept of Biology, Johns Hopkins University Post Doctoral Fellow, Dept of Biology, Johns Hopkins University

研究领域

The Geyer laboratory is interested in understanding mechanisms involved in genome organization and gene regulation. Two areas are currently being studied, insulators and nuclear lamina function. Insulators are a specialized class of DNA regulatory elements that have a conserved role in chromosome organization, dividing chromosomes into independent functional domains. These elements shelter genes from the surrounding positive and negative regulatory effects by blocking interactions between enhancers, silencers and promoters. Such properties have made insulators favorable candidates to improve strategies for gene therapy. While gene therapy is a promising technology that delivers therapeutic genes to a target tissue, successful application of this approach faces many challenges, including the cross regulation of endogenous genes by DNA regulatory elements in the vector. Insulators may prevent such undesirable effects. Studies in the Geyer lab include determination of the molecular mechanisms used by insulators to prevent interactions between enhancers, silencers and promoters and the identification of novel insulators. Combinations of insulators that employ different regulatory mechanisms may provide optimal protection of transgenes from the position effects of the surrounding chromatin and prevent sequences in the vector from mis-regulating endogenous genes at the site of genomic insertion. As such, insights into insulator mechanisms will allow advances in improving strategies for gene therapy and treatment of human disease. The nuclear lamina is an extensive protein network that lines the inner surface of the nuclear membrane. This network consists of intermediate filament proteins, called lamins, and associated inner nuclear membrane proteins, such as the LEM domain proteins. LEM domain genes make essential contributions to the function of the lamina, as mutations in these genes cause human diseases, known as laminopathies. For example, mutations in the genes that encode emerin and MAN1 cause Emery-Dreifuss Muscular Dystrophy and Buschke-Ollendorf Syndrome, respectively. Interestingly, these diseases are associated with tissue-specific pathologies, even though LEM domain genes are broadly expressed. To understand mechanisms underlying such human diseases, the Geyer lab is investigating the role of LEM domain proteins in lamina function, using Drosophila as a model organism. A molecular genetic approach is being taken to determine the contributions that individual LEM domain proteins make to nuclear lamina function.

近期论文

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arton, L. J., Lovanr, K. ., into, . S. & Gyr, . K. (2016). rosohila al an fal grlin st cll nichs rqir th nclar laina rotin Otfin.. vlontal iology, 415(1), 75-86. OI: 10.1016/j.yio.2016.05.001. arton, L. J., Soshnv, A. A. & Gyr, . K. (2015). Ntworking in th ncls: a sotlight on L-oain rotins.. Crrnt oinion in cll iology, 34, 1-8. OI: 10.1016/j.c.2015.03.005. arton, L. J., Wilington, S. R., artin, . J., Skoc, H. ., Lovanr, K. ., into, . S. & Gyr, . K. (2014). niq an shar fnctions of nclar laina L oain rotins in rosohila.. Gntics, 197(2), 653-65. OI: 10.1534/gntics.114.162941. arton, L. J., into, . S., Wallrath, L. L. & Gyr, . K. (2013). Th rosohila nclar laina rotin otfin is rqir for grlin st cll srvival. vlontal cll, 25(6), 645-54. Soshnv, A. A., axly, R. ., anak, J. R., Tan, K. & Gyr, . K. (2013). Th inslator rotin Srssor of Hairy-wing is an ssntial transcritional rrssor in th rosohila ovary. vlont, 140(17), 3613-23. Hohl, A. ., Thoson, ., Soshnv, A. A., W, J., orris, J., Hsih, T. S., W, C. T. & Gyr, . K. (2012). Rstoration of tooisoras 2 fnction y colntation of fctiv onors in rosohila. Gntics, 192(3), 843-56. Shoshnv, A. A., H, ., axly, R. ., Jiang, N., Hart, C. ., Tan, K. & Gyr, . K. (2012). Gno-wi stis of th lti-zinc fingr rosohila Srssor of Hairy-wing rotin in th ovary. Nclic Acis Rsarch, 40(12). OI: 10.1093/nar/gks225. axly, R. ., Soshnv, A. A., Koryakov, . ., Zhilv, I. F. & Gyr, . K. (2011). Th rol of th Srssor of Hairy-wing inslator rotin in rosohila oognsis. vlontal iology, 356(2), 398-410. Gyr, . K., Vitalini, . W. & Wallrath, L. L. (2011). Nclar organization: taking a osition on gn xrssion. Crrnt oinion in cll iology, 23(3), 354-9. ialynas, G., Ss, S., nik, V., Gyr, . K. & Wallrath, L. L. (2010). Th rol of rosohila Lain C in scl fnction an gn xrssion. vlont (Carig, nglan), 137(18), 3067-77.