研究领域

In our lab we study the mechanisms of meiotic chromosome segregation, focusing on the events that ensue at meiotic prophase I. We are particularly interested in elucidating the mechanisms of SC disassembly. For our studies of meiosis, we use Caenorhabditis elegans as a model system (a). C. elegans is a microscopic worm, which is a well-established model organism amenable for a variety of research approaches including genetic manipulation, cell biology and biochemistry. We can analyze chromosomes in the live organisms as well as in dissected gonads. Meiotic prophase nuclei are the majority of nuclei composing the germline of this microscopic worm (b). These nuclei are arranged in temporal-spatial pattern; therefore, all early meiotic events (c-e) can be analyzed simultaneously in a single gonad. We use genetic and cell biology techniques involving high-resolution microscopy to study how chromosomes behave in these early meiotic events.

Cell and Developmental Biology, Genetics

近期论文

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Reichman R, Alleva B, Smolikove S. 2017. Prophase I: Preparing Chromosomes for Segregation in the Developing Oocyte.. Results Probl Cell Differ. 59:125-173. Review Alleva B, Balukoff N, Peiper A, Smolikove S. 2017. Regulating chromosomal movement by the cochaperone FKB-6 ensures timely pairing and synapsis. Journal of Cell Biology. 216(2):393-408 Yin Y, Donlevy S, Smolikove S. 2016. Coordination of Recombination with Meiotic Progression in the Caenorhabditis elegans Germline by KIN-18, a TAO Kinase That Regulates the Timing of MPK-1 Signaling.. Genetics. 202(1):45-59 Brockway HM, Balukoff N, Dean M, Alleva B, Smolikove S. 2014. Signalosome Regulates Synaptonemal Complex Assembly during Meiotic Prophase I of Caenorhabditis elegans.. Plos Genetics. 10(11)journal.pgen_.1004757.pdf (1.9 MB) Clemons AM, Brockway HM, Yin Y, Kasinathan B, Butterfield YS, Jones SJM, Colaiácovo MP, Smolikove S. 2013. akirin is required for diakinesis bivalent structure and synaptonemal complex disassembly at meiotic prophase I.. Mol Biol Cell. 24(7):1053-67. Clemons et al 2013.pdf (6.27 MB) Yin Y, Smolikove S. 2013. Impaired resection of meiotic double-strand breaks channels repair to nonhomologous end joining in Caenorhabditis elegans.. Mol Cell Biol. 33(14):2732-47. Schild-Prüfert K, Saito TT, Smolikov S, Gu Y, Hincapie M, Hill DE, Vidal M, McDonald K, Colaiácovo MP. 2011. Organization of the synaptonemal complex during meiosis in Caenorhabditis elegans.. Genetics. 189(2):411-21. SC.pdf (2.97 MB) Smolikov S, Schild-Prüfert K, Colaiácovo MP. 2009. A yeast two-hybrid screen for SYP-3 interactors identifies SYP-4, a component required for synaptonemal complex assembly and chiasma formation in Caenorhabditis elegans meiosis.. PLoS Genet. 5(10):e1000669. SYP-4.pdf (651.02 KB) Smolikov S, Schild-Prüfert K, Colaiácovo MP. 2008. CRA-1 uncovers a double-strand break-dependent pathway promoting the assembly of central region proteins on chromosome axes during C. elegans meiosis.. PLoS Genet. 4(6):e1000088. CRA-1.pdf (862.99 KB) de Carvalho C E, Zaaijer S, Smolikov S, Gu Y, Schumacher JM, Colaiácovo MP. 2008. LAB-1 antagonizes the Aurora B kinase in C. elegans.. Genes Dev. 22(20):2869-85. LAB-1.pdf (1.72 MB)