Griffin, Robert Guy - Massachusetts Institute of Technology

研究领域

The second major part of our research is the application of the magnetic resonance techniques described above to interesting chemical, biophysical, and physical problems. We are currently employing MAS NMR experiments to investigate the structure of large enzyme/inhibitor complexes, membrane proteins and amyloid peptide and proteins. By measuring chemical shifts and dipolar couplings between homonuclear and heteronuclear spin pairs in inhibitors or active sites, we have been able to address structural questions in several cases. For example, we have performed NMR on photochemical intermediates of bacteriorhodopsin trapped at low temperature to study the mechanism of proton pumping and the origin of the opsin shift in the optical spectrum of retinal in this protein. More recently we have completed the initial two structures of peptides with MAS dipole recoupling techniques. One of these, an 11-mer from the protein transthyretin, is illustrated in the bottom part of the figure and is the initial structure of a peptide in an amyloid fibril. Since these molecules do not diffract and are not soluble, their structures can only be determined by high resolution solid state NMR.

近期论文

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Adiabatic Solid Effect, K. Ooi Tan, Weber, R. T., Can, T. V., and Griffin, R. G., , The Journal of Physical Chemistry LettersThe Journal of Physical Chemistry Letters, pp. 3416 - 3421, 2020. Pulsed Dynamic Nuclear Polarization, K. Ooi Tan, Jawla, S., Temkin, R. J., and Griffin, R. G., , eMagRes, pp. 339-352, 2019. Modular, triple–resonance, transmission line DNP MAS probe for 500 MHz/330 GHz, M. Reese, George, C., Yang, C., Jawala, S., J. Grün, T., Schwalbe, H., Redfield, C., Temkin, R. J., and Griffin, R. G., , p. 106573, 2019. High frequency dynamic nuclear polarization: New directions for the 21st century, R. G. Griffin, Swager, T. M., and Temkin, R. J., , Journal of Magnetic Resonance, 2019. Three-spin solid effect and the spin diffusion barrier in amorphous solids, K. Ooi Tan, Mardini, M., Yang, C., Ardenkjær-Larsen, J. Henrik, and Griffin, R. G., , Science Advances, vol. 5, 2019. High Resolution 17O NMR Spectroscopy of Structural Water, E. G. Keeler, Michaelis, V. K., Wilson, C. B., Hung, I., Wang, X., Gan, Z., and Griffin, R. G., , The Journal of Physical Chemistry B, 2019. Convolutional Neural Network Analysis of Two-Dimensional HYSCORE EPR Spectra, A. T. Taguchi, Evans, E. D., Dikanov, S. A., and Griffin, R. G., , The Journal of Physical Chemistry Letters, 2019. Time-optimized pulsed dynamic nuclear polarization, K. Ooi Tan, Yang, C., Weber, R. T., Mathies, G., and Griffin, R. G., , Science Advances, vol. 5, p. eaav6909, 2019. Conformation of bis-nitroxide polarizing agents by multi-frequency EPR spectroscopy, J. Soetbeer, Gast, P., Walish, J. J., Zhao, Y., George, C., Yang, C., Swager, T. M., Griffin, R. G., and Mathies, G., , Physical Chemistry Chemical Physics, vol. 20, pp. 25506–25517, 2018. Metal-free class Ie ribonucleotide reductase from pathogens initiates catalysis with a tyrosine-derived dihydroxyphenylalanine radical, E. J. Blaesi, Palowitch, G. M., Hu, K., Kim, A. J., Rose, H. R., Alapati, R., Lougee, M. G., Kim, H. Jong, Taguchi, A. T., Tan, K. Ooi, Laremore, T. N., Griffin, R. G., Krebs, C., Matthes, M. L., Silakov, A., J. Jr., M. Bollinger, Allen, B. D., and Boal, A. K., , Proceedings of the National Academy of Sciences, vol. 115, pp. 10022–10027, 2018. The structure of a β2-microglobulin fibril suggests a molecular basis for its amyloid polymorphism, M. G. Iadanza, Silvers, R., Boardman, J., Smith, H. I., Karamanos, T. K., Debelouchina, G. T., Su, Y., Griffin, R. G., Ranson, N. A., and Radford, S. E., , Nature Communications, vol. 9, 2018. Primary Transfer Step in the Light-Driven Ion Pump Bacteriorhodopsin: An Irreversible U-Turn Revealed by Dynamic Nuclear Polarization-Enhanced Magic Angle Spinning NMR, Q. Zhe Ni, Can, T. V., Daviso, E., Belenky, M., Griffin, R. G., and Herzfeld, J., , Journal of the American Chemical Society, vol. 140, no. 11, pp. 4085 - 4091, 2018. High-precision measurement of the electron spin g factor of trapped atomic nitrogen in the endohedral fullerene N@C60, J. J. Wittmann, Can, T. V., Eckardt, M., Harneit, W., Griffin, R. G., and Corzilius, B., , Journal of Magnetic Resonance, vol. 290, pp. 12 - 17, 2018. Frequency-Swept Integrated and Stretched Solid Effect Dynamic Nuclear Polarization, T. V. Can, McKay, J. E., Weber, R. T., Yang, C., Dubroca, T., van Tol, J., Hill, S., and Griffin, R. G., , The Journal of Physical Chemistry Letters, pp. 3187 - 3192, 2018. Overhauser effects in non-conducting solids at 1.2 K, X. Ji, Can, T. V., Mentink-Vigier, F., Bornet, A., Milani, J., Vuichoud, B., Caporini, M. A., Griffin, R. G., Jannin, S., Goldman, M., and Bodenhausen, G., , Journal of Magnetic Resonance, vol. 286, pp. 138 - 142, 2018. 17O MAS NMR Correlation Spectroscopy at High Magnetic Fields, E. G. Keeler, Michaelis, V. K., Colvin, M. T., Hung, I., Gor’kov, P. L., Cross, T. A., Gan, Z., and Griffin, R. G., , Journal of the American Chemical Society, vol. 139, pp. 17953-17963, 2017. Proton-Assisted Recoupling (PAR) in Peptides and Proteins, K. J. Donovan, Jain, S. K., Silvers, R., Linse, S., and Griffin, R. G., , The Journal of Physical Chemistry B, vol. 121, pp. 10804-10817, 2017. Off-resonance NOVEL, S. K. Jain, Mathies, G., and Griffin, R. G., , The Journal of Chemical Physics, vol. 147, p. 164201, 2017. Aggregation and Fibril Structure of AβM01-42 and Aβ1-42, R. Silvers, Colvin, M. T., Frederick, K. K., Jacavone, A. C., Lindquist, S. L., Linse, S., and Griffin, R. G., , Biochemistry, vol. 56, no. 36, pp. 4850-4859, 2017.