Southern, Cathrine A. - DePaul University

研究领域

The examination of antibody structure via single molecule Förster resonance energy transfer (FRET). This technique involves incorporating two dye molecules into a protein of interest at known locations. The distance between the two dye molecules can be determined based on their relative fluorescence intensities. By examining the fluorescence from individual proteins labeled with the dye molecules, a histogram of the observed distances between the dyes can be formulated. This histogram allows the presence of multiple protein conformations to be detected. The primary goal of Southern's research is to determine whether or not distinct antibody conformations exist. Southern is interested in antibody structure because it can be affected by mutations in the antibody, and can influence the ability of an antibody to instigate an immune response. In some cases, increasing an immune response is the desired effect; in others, such as autoimmune disorders, diminishing an immune response is preferred. Studying the structure of antibodies and how this structure is affected by mutations or the removal of sugars bound to antibodies can shed light on the role these structural changes play in generating an immune response.

近期论文

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“The Effect of Sugar Removal on the Structure of the Fc region of an IgG Antibody as Observed with Single Molecule F?rster Resonance Energy Transfer,” M. T. Kelliher, R. D. Jacks, M. S. Piraino, C. A. Southern, Molecular Immunology, 2014, 60, 103. link “A Comparison of F?rster Resonance Energy Transfer Analysis Approaches for Nanodrop Fluorometry,” M. T. Kelliher, M. S. Piraino, M. E. Gemoules, C. A. Southern, Analytical Biochemistry, 2013, 441, 44. link “Genetic and Fluorescence Studies of Affinity Maturation in Related Antibodies,” T. Pauyo, G. J. Hilinski, P. T. Chiu, D. E. Hansen, Y. J. Choi, D. I. Ratner, N. Shah-Mahoney, C. A. Southern, P. B. O’Hara, Molecular Immunology, 2006, 43, 812. link “Steady State and Multifrequency Phase Fluorometry Studies of Binding Site Flexibility in Related Antibodies,” G. S. Mohan, P. T. Chiu, C. A. Southern, P. B. O’Hara, Journal of Physical Chemistry A, 2004, 108, 7871. link “Spectroscopic Consequences of Localized Electronic Excitation in Anthranilic Acid Dimer,” C. A. Southern, D. H. Levy, J. A. Stearns, G. M. Florio, A. Longarte, T. S. Zwier, Journal of Physical Chemistry A, 2004, 108, 4599. link “Electronic and Infrared Spectroscopy of Anthranilic Acid in a Supersonic Jet,” C. A. Southern, D. H. Levy, G. M. Florio, A. Longarte, T. S. Zwier, Journal of Physical Chemistry A, 2003, 107, 4032. link