个人简介

Pat Limbach is a Professor and Ohio Eminent Scholar at the University of Cincinnati. He is a bioanalytical chemist with research interests in mass spectrometry, modified ribonucleic acids (RNAs), ribonucleoprotein (RNP) complexes and protein translation. After earning an undergraduate degree from Centre College in 1988, he studied under the direction of Dr. Alan G. Marshall at The Ohio State University. While there, his graduate research focused on instrumentation improvements to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. He received his PhD from OSU in 1992. He then took a postdoctoral position at the University of Utah working with Dr. James A. McCloskey. While in Utah, he worked in the areas of RNA chemistry and nucleic acid mass spectrometry. In 1995, he joined the faculty at Louisiana State University as an Assistant Professor and was promoted to Associate Professor in 1999. In 2001, he moved to his current position in Cincinnati, being promoted to Professor in 2005. He has served as Department Head in Chemistry at UC from 2005-2010 and as Interim Associate Dean for Research and Graduate Studies in the McMicken College of Arts and Sciences in 2013. Dr. Limbach and his group seek to advance the area of mass spectrometry within modified RNAs and RNPs and collaborate extensively with researchers inside the state of Ohio and throughout the world. Dr. Limbach is an active member of the American Chemical Society, American Society for Mass Spectrometry, Sigma Xi and a lifetime member of Phi Kappa Phi. He also serves on the Scientific Advisory Board for RiboNova, Inc.

研究领域

mass spectrometry modified nucleosides transfer RNAs structural investigation of nucleic acids characterization of ribonucleoprotein complexes assays for therapeutic oligonucleotides nucleic acid biomarkers in humans

The primary interest of Prof. Pat Limbach and members of his research group is to develop new and improved mass spectrometric methods to characterize modified ribonucleic acids (RNAs) and ribonucleoprotein complexes (RNPs). As such, we have active research programs involved in nucleic acid analysis by mass spectrometry, structural biology and medical applications. Details can be accessed below. Sequencing of Modified RNAs by Mass Spectrometry Biological Studies of Modified RNA Nucleosides Structural Characterization of RNA:Protein Complexes Medical Research Impacts of Modified RNAs

近期论文

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Gaston, K.W.; Limbach, P.A.; “Mass Spectrometry for the Identification and Characterization of Modified Nucleosides and Nucleic Acids” RNA Biology (2014) 11 1568-1585. PMID: 25616408. [Link] Mummadisettia, M.P.; Frankel, L.K.; Bellamy, H.; Sallans, L.; Goettertb, J.S.; Brylinski, M.; Limbach, P.A.; Bricker, T.M.; “Use of protein crosslinking and radiolytic footprinting to elucidate PsbP and PsbQ interactions within higher plant Photosystem II” Proceedings of the National Academy of Sciences, USA. 111 (2014) 16178-16183. PMID: 25349426. [Link] Wilson, Z.; McGuire, S.; Limbach, P.A.; Doyle, M.; Marzilli, L.; Warner, I.M.; “Diversifying Science, Technology, Engineering, and Mathematics – An empirical inquiry into successful approaches in chemistry” Journal of Chemical Education 91 (2014) 1860–1866. DOI: 10.1021/ed400702v. [Link] Li, S.; Limbach, P.A.; “Identification of RNA Sequence Isomers by Isotope Labeling and LC-MS/MS” Journal of Mass Spectrometry 49 (2014) 1191-1198. DOI: 10.1002/jms.3449. PMID: 25395135. [Link] Puri, P.; Wetzel, C.; Saffert, P.; Gaston, K.W.; Russell, S.P.; van der Vlies, P.; Zhang, G.; Limbach, P.A.; Ignatova, Z.; Poolman, B.; “Systematic identification tRNAome and its dynamics in Lactococcus lactis.” Molecular Microbiology 93 (2014) 944-956. DOI: 10.1111/mmi.12710. PMID: 25040919. [Link] Zallot, R.; Brochier-Armanet, C.; Gaston, K.W.; Forouhar, F.; Limbach, P.A.; Hunt, J.; de Crécy-Lagard, V.; “The plant, animal, and fungal micronutrient queuosine is salvaged by members of the DUF2419 protein family”, ACS Chemical Biology 9 (2014) 1812-1825. DOI: 10.1021/cb500278k. PMID: 24911101. [Link] Dator, R.P.; Gaston, K.W.; Limbach, P.A.; “Multiple Enzymatic Digestions and Ion Mobility Separation Improve Quantification of Bacterial Ribosomal Proteins by Data Independent Acquisition Liquid Chromatography−Mass Spectrometry” Analytical Chemistry 86 (2014) 4264-4270. DOI:10.1021/ac404020j PMID: 24738621. PMCID: PMC4014174. [Link] Wetzel, C.; Li, S.; Limbach, P.A.; “Metabolic De-isotoping for Improved LC-MS Characterization of Modified RNAs” Journal of the American Society for Mass Spectrometry 25 (2014) 1114-1123. DOI:10.1007/s13361-014-0889-9 PMID: 24760295. [Link] Köhrer, C.; Mandal, D.; Gaston, K.W.; Grosjean, H.; Limbach, P.A.; RajBhandary, U.L.; “Life without tRNAIle-lysidine synthetase: Translation of the isoleucine codon AUA in Bacillus subtilis lacking the canonical tRNA2Ile“ Nucleic Acids Research 42 (2014) 1904-1915 PMID: 24194599. [Link] Wong, S.Y.; Javid, B.; Addepalli, B.; Piszczek, G.; Strader, M.B.; Limbach, P.A.; Barry III, C.E. “Functional Role of Methylation of G518 of the 16S rRNA 530 loop by GidB in Mycobacterium tuberculosis” Antimicrobial Agents and Chemotherapy 57 (2013) 6311-6318. DOI:10.1128/AAC.00905-13. PMID: 24100503. [Link] Clatterbuck Soper, S.F.; Dator, R.P.; Limbach, P.A.; Woodson, S.A.; “In vivo X-ray footprinting of pre-30S ribosomes reveals chaperone-dependent remodeling of late assembly intermediates”, Molecular Cell 52 (2013) 506-516. DOI:10.1016/j.molcel.2013.09.020. PMID: 24207057. [Link] Comment in Pyle, A.M. “Coordinating the Party: Assembly Factors and Ribogenesis” Molecular Cell, 52 (2013) 469-470. [Link] Wetzel, C.; Limbach, P.A.; “The Global Identification of tRNA Isoacceptors by Targeted Tandem Mass Spectrometry”, Analyst 138 (2013) 6063-6072. DOI:10.1039/c3an01224g. PMID: 23954863. [Link] Rodriguez-Hernandez, A.; Spears, J.; Gaston, K.W.; Limbach, P.A.; Gamper, H.; Hou, Y.-M.; Agris, P.; Perona, J.J.; “Structural and mechanistic basis for enhanced translational efficiency by 2-thiouridine at the tRNA anticodon wobble position” Journal of Molecular Biology 425 (2013) 3888-3906. DOI:10.1016/j.jmb.2013.05.018. PMID: 23727144. [Link] Nayak, N.; Putnam, A.; Chen, T.; Schäfermeyer, K.R.; Addepalli, B.; Lowenson, J.; Patra, B.; Jankowsky, E.; Perry, S.E.; Dinkins, R.; Limbach, P.A.; Ray, A.; Clarke, S.G.; Downie, B.; “The DEAD-box RNA helicase, PRH75, is susceptible to isoAsp formation but can be repaired and activity restored by PROTEIN ISOASPARTYL METHYLTRANSFERASE”, The Plant Cell 25 (2013) 2573-86. DOI:10.1105/tpc.113.113456. PMID: 23903319. [Link] Frankel, L.K.; Sallans, L.; Bellamy, H.; Goettert, J.S.; Limbach, P.A.; Bricker, T.M.; “Radiolytic Mapping of Solvent Contact Surfaces in Photosystem II of Higher Plants: Experimental Identification of Putative Water Channels within the Photosystem” Journal of Biological Chemistry 288 (2013) 23565-23572. DOI:10.1074/jbc.M113.487033. PMID: 23814046. [Link] Easter, R.N.; Barry, C.G.; Caruso, J.A.; Limbach, P.A.; “Separation and Identification of Phosphorothioate Oligonucleotides by Hydrophilic Interaction Liquid Chromatography Coupled to Electrospray Ionization Mass Spectrometry”, Analytical Methods 5 (2013) 2657-2659. DOI:10.1039/C3AY26519F. [Link] Russell, S.P.; Limbach, P.A.; “Evaluating the Reproducibility of Quantifying Modified Nucleosides from Ribonucleic Acids by LC-UV-MS” Journal of Chromatography, B 923–924 (2013)74-82. DOI:10.1016/j.jchromb.2013.02.010. PMID: 23500350. [Link] Frankel, L.K.; Sallans, L.; Limbach, P.A.; Bricker, T.M.; “Oxidized Amino Acid Residues in the Vicinity of QA and PheoD1 of the Photosystem II Reaction Center: Putative Generation Sites of Reducing-Side Reactive Oxygen Species” PLoS One. 8 (2013) e58042. doi:10.1371/journal.pone.0058042. PMID: 23469138. PMCID: PMC3585169. [Link] Li, S.; Limbach, P.A.; “Comparative Sequencing of Transfer Ribonucleic Acids by Isotope Labeling and Mass Spectrometry”, Analyst 138 (2013) 1386-1394. DOI:10.1039/C2AN36515D. PMID: 23295341. [Link]