李寅青 - 清华大学

个人简介

李寅青博士目前受聘于清华大学，任特别研究员、博士生导师。他于2008年本科毕业于复旦大学微电子系，并于2012年和2016年在麻省理工学院获得电子工程与计算机专业硕士和博士学位。在2016至2018年在博德研究所、斯坦利精神疾病中心完成博士后研究。在博士和博士后期间，与导师Feng Zhang博士、Aviv Regev博士和Guoping Feng博士合作，从事基因组学和工程学的交叉领域的研究，特别是单细胞的基因检析、基因编辑和调控技术的建立。李寅青博士致力于开发和拓展基因组医学技术，以推动复杂疫病如神经退行性病变、肿瘤的新药靶点发现和精确诊疗。荣誉和奖项 2019 MIT Technology Review TR35 China 2016 国家优秀自费留学生，特别优秀奖 2016 Wenner-Gren Fellowship 2013 McGovern Institute Fellowship

研究领域

复杂疾病的产生和发展往往缘起并伴随着一系列发生在分子和细胞层面的关键变化。诊断和治疗的重要途径即是要理解在疾病的产生和发展过程中，哪个时间点上有哪些分子和细胞发生了关键变化，以及这些变化是如何影响细胞自身和细胞间互相作用的，并通过针对关键分子和细胞的编辑，实现对于疾病的控制。李寅青博士的研究团队专注于基因组医学技术的三个重要方面的开发。（1）单细胞表征技术。实现表观遗传组、转录组、信号组单细胞多组学，在时间和空间两个尺度上整体表征分子、细胞、细胞间作用的变化（2）基因编辑技术。探索新的编辑系统，基因替换和表观遗传控制，实现细胞从基因到状态的全面调控（3）生物信息学。发展与应用统计推断及机器学习算法，分析高通量数据和挖掘基因改造工具

近期论文

查看导师新发文章（温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

Hu Y, Yang Y, Tan P, Zhang Y, Han M, Yu J, Zhang X, Jia Z, Wang D, Li Y, Ma T, Liu K, Ding S. Induction of mouse totipotent stem cells by a defined chemical cocktail. NATURE. 2022 Jun 21. Tan Y, Shen J, Si T, Ho CL, Li Y, Dai L. Engineered Live Biotherapeutics: Progress and Challenges. BIOTECHNOLOGY JOURNAL 2020 Oct;15(10):e2000155. Du J, Simmons S, Brunklaus A, Adiconis X, Hession CC, Fu Z, Li Y, Shema R, M?ller RS, Barak B, Feng G, Meisler M, Sanders S, Lerche H, Campbell AJ, McCarroll S, Levin JZ, Lal D. Differential excitatory vs inhibitory SCN expression at single cell level regulates brain sodium channel function in neurodevelopmental disorders. EUROPEAN JOURNAL OF PAEDIATRIC NEUROLOGY 2020 Jan;24:129-133. Li Y, Lopez-Huerta VG, Adiconis X, Levandowski K, Choi S, Simmons SK, Arias-Garcia MA, Guo B, Yao AY, Blosser TR, Wimmer RD, Aida T, Atamian A, Naik T, Sun X, Bi D, Malhotra D, Hession CC, Shema R, Gomes M, Li T, Hwang E, Krol A, Kowalczyk M, Pe?a J, Pan G, Halassa MM, Levin JZ, Fu Z, Feng G. Distinct subnetworks of the thalamic reticular nucleus. NATURE. 2020 Jul;583(7818):819-824. Li Y, Weiss R., A Modular Approach to Building Complex Synthetic Circuits. Methods in Molecular Biology. 2017 pp. 231–248, DOI:10.1007/978-1-4939-7223-4_17. Yan WX, Mirzazadeh R, Garnerone S, Scott D, Schneider MW, Kallas T, Custodio J, Wernersson E, Li Y, Gao L, Federova Y, Zetsche B, Zhang F, Bienko M, Crosetto N., BLISS is a versatile and quantitative method for genome-wide profiling of DNA double-strand breaks. NATURE COMMUNICATIONS. 2017, 8:15058. Habib N*, Li Y*, Heidenreich M, Swiech L, Avraham-Davidi I, Trombetta JJ, Hession C, Zhang F, Regev A. Div-Seq: Single-nucleus RNA-Seq reveals dynamics of rare adult newborn neurons. SCIENCE. 2016, 353(6302):925-8. Yamano T, Nishimasu H, Zetsche B, Hirano H, Slaymaker IM, Li Y, Fedorova I, Nakane T, Makarova KS, Koonin EV, Ishitani R, Zhang F, Nureki O. Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA. CELL. 2016, 165(4):949-62. Nishimasu H, Cong L, Yan W, Ran F, Bernd Z, Li Y, Kurabayashi A, Ishitani R, Zhang F, Nureki O. Crystal structure of Staphylococcus aureus Cas9. CELL. 2015, 162(5):1113-26. Davidsohn N, Beal J, Kiani S, Adler A, Yaman F, Li Y, Xie Z, Weiss R. Accurate predictions of genetic circuit behavior from part characterization and modular composition. ACS SYNTHETIC BIOLOGY. 2015, 4(6):673-81. Li Y*, Jiang Y*, Chen H*, Liao W, Li Z, Weiss R, Xie Z. Modular construction of mammalian gene circuits using TALE transcriptional repressors. NATURE CHEMICAL BIOLOGY. 2015, 11(3):207-13. Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. NATURE BIOTECHNOLOGY. 2015, 33(1):102-6. Duportet X, Wroblewska L, Guye P, Li Y, Eyquem J, Rieders J, Rimchala T, Batt G, Weiss R. A platform for rapid prototyping of synthetic gene networks in mammalian cells. NUCLEIC ACIDS RESEARCH. 2014, 42(21):13440-51. Busskamp V, Lewis NE, Guye P, Ng AH, Shipman SL, Byrne SM, Sanjana NE, Murn J, Li Y, Li S, Stadler M, Weiss R, Church GM. Rapid neurogenesis through transcriptional activation in human stem cells. MOLECULAR SYSTEMS BIOLOGY. 2014, 10:760. Kiani S, Beal J, Ebrahimkhani MR, Huh J, Hall RN, Xie Z, Li Y, Weiss R. CRISPR transcriptional repression devices and layered circuits in mammalian cells. NATURE METHODS. 2014, 11(7):723-6. Hsu PD, Scott DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O, Cradick TJ, Marraffini LA, Bao G, Zhang F. DNA targeting specificity of RNA-guided Cas9 nucleases. NATURE BIOTECHNOLOGY. 2013, 31(9):827-32. Guye P*, Li Y*, Wroblewska L, Duportet X, Weiss R. Rapid, modular and reliable construction of complex mammalian gene circuits. NUCLEIC ACIDS RESEARCH. 2013, 41(16):e156. Sakar MS, Neal D, Boudou T, Borochin MA, Li Y, Weiss R, Kamm RD, Chen CS, Asada HH. Formation and optogenetic control of engineered 3D skeletal muscle bioactuators. LAB ON A CHIP. 2012, 12(23):4976-85. Zhao P, Li Y, Zeng X, Zhou J, Huang Y, Liu R. EWOD using P(VDF-TrFE). IEEE Nano/Micro Engineered and Molecular Systems. 2009, 10.1109/NEMS.2009.5068559. Zhao P, Li Y, Zhou J, Huang Y, Xie H, Xu H. A novel EWOD digital microuidic device using P(VDF-TrFE). IEEE Solid-State and Integrated-Circuit Technology. 2008, 10.1109/ICSICT.2008.4735080.