研究领域

bio-organic chemistry enzyme engineering biocatalysis biodetection drug discovery

Professor Pelletier’s research is aimed at modifying enzymes, particularly in their active sites, and overcoming resistance to antibiotics, to provide new industrial applications in biocatalysis and green chemistry, and increase our understanding of enzyme catalysis.

近期论文

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Zhao SS, Bukar N, Toulouse JL, Pelechacz D, Robitaille R, Pelletier JN, Masson JF. (2014) Miniature multi-channel SPR instrument for methotrexate monitoring in clinical samples. Biosens. Bioelectron. 64, 664-670 Gobeil SM, Clouthier CM, Park J, Gagné D, Berghuis AM, Doucet N, Pelletier JN. (2014) Maintenance of native-like protein dynamic may not be required for engineering functional proteins. Chem Biol. 21(10), 1330-1340 Article previewed in: Tokuriki N, Jackson CJ (2014) Enzyme dynamics and engineering: one step at a time, Chem Biol. 21(10), 1259-1260 Bukar N, Zhao SS, Charbonneau DM, Pelletier JN, Masson JF. (2014) Influence of the Debye length on the interaction of small molecule-modified nanoparticle with a surface-bound bioreceptor. Chem Commun (Camb). 18;50(38) 4947-4950 Aubé A, Breault-Turcot J, Chaurand P, Pelletier JN, Masson JF. (2013) Non-specific adsorption of crude cell lysate on surface plasmon resonance sensors. Langmuir. 2013 Aug 13;29(32):10141-8. doi: 10.1021/la401837y. Ratel M, Provencher-Girard A, Zhao SS, Breault-Turcot J, Labrecque-Carbonneau J, Branca M, Pelletier JN, Schmitzer AR, Masson JF. (2013) Imidazolium-based ionic liquid surfaces for biosensing. Anal Chem. 2013 Jun 18;85(12):5770-7. doi: 10.1021/ac400386z. Gundersen MT, Keillor JW, Pelletier JN. (2013) Microbial transglutaminase displays broad acyl-acceptor substrate specificity. Appl Microbiol Biotechnol. 2013 Apr 25. Rachel NM, Pelletier JN. (2013) Biotechnological Applications of Transglutaminases. Biomolecules 2013, 3, 870-888. doi:10.3390/biom3040870. Clouthier CM, Morin S, Gobeil SM, Doucet N, Blanchet J, Nguyen E, Gagné SM, Pelletier JN. (2012) Chimeric β-lactamases: global conservation of parental function and fast time-scale dynamics with increased slow motions. PLoS One. 2012;7(12):e52283. Zhao, S. S., Bichelberger, M. A., Colin, D. Y., Robitaille, R., Pelletier, J. N., and Masson, J. F. (2012) Monitoring methotrexate in clinical samples of cancer patients during chemotherapy with a LSPR-based competitive sensor. Analyst 2012, 137, 4742-4750. Gnaccarini, C., Ben-Tahar, W., Mulani, A., Roy, I., Lubell, W. D., Pelletier, J. N. and Keillor, J. W. Site-Specific Protein Propargylation Using Tissue Transglutaminase (2012) Org. Biomol. Chem. DOI: 10.1039/C2OB25752A. Bastien, D., Ebert, M. C. C. J. C., Forge,D., Toulouse, J., Kadnikova, N., Perron, F., Mayence, A., Huang, T. L., Vanden Eynde, J. J., and Pelletier, J. N. (2012) Fragment-Based Design of Symmetrical Bis-benzimidazoles as Selective Inhibitors of the Trimethoprim-Resistant, Type II R67 Dihydrofolate Reductase. J. Med. Chem. 55, 3182-3192. Clouthier, C. M., and Pelletier, J.N. (2012) Expanding the organic toolbox: a guide to integrating biocatalysis in synthesis. Chem. Soc. Rev. 41, 1585–1605. Yachnin, B. J.; Colin, D. Y.; Volpato, J. P.; Ebert, M.; Pelletier, J. N.; Berghuis, A. M. (2011) Novel crystallization conditions for tandem variant R67 DHFR yield a wild-type crystal structure. Acta Crystallogr., Sect. F: Struct. Biol. Cryst. Commun. 67, 1316-1322. Bolduc, O. R., Lambert-Lanteigne, P., Colin, D.P., Shuo Zhao, S., Proulx, C., Boeglin, D., Lubell, W., Pelletier, J. N., Féthière, J., Ong, H. and Masson, J.-F. (2011) Peptidomimetic monolayer binding His-tagged biomolecules for small ligand screening with SPR biosensors. Analyst, 136, 3142-3148. Tang, M-C., Nisole, A., Dupont, C., Pelletier, J.N. and Waldron, K.C. (2011) Chemical profiling of the deacetylase activity of acetyl xylan esterase A (AxeA) variants on chitooligosaccharides using hydrophilic interaction chromatography-mass spectrometry. J. Biotechnol. 155 257– 265 Volpato J.P., Mayotte N., Fossati E., Guerrero V., Sauvageau G., Pelletier J.N. (2011) Selectively weakened binding of methotrexate by human dihydrofolate reductase allows rapid ex vivo selection of mammalian cells. J Mol Recognit., 24 (2):188-98. Banka S., Blom H.J., Walter J., Aziz M., Urquhart J., Clouthier C.M., Rice G.I., de Brouwer A.P., Hilton E., Vassallo G., Will A., Smith D.E., Smulders Y.M., Wevers R.A., Steinfeld R., Heales S., Crow Y.J., Pelletier J.N., Jones S., Newman W.G. (2011) Identification and characterization of an inborn error of metabolism caused by dihydrofolate reductase deficiency. Am J Hum Genet., 88 (2):216-25. Morin S., Clouthier C.M., Gobeil S., Pelletier J.N., Gagné S.M. (2010) Backbone resonance assignments of an artificially engineered TEM-1/PSE-4 Class A beta-lactamase chimera. Biomol NMR Assign., 4 (2):127-30 Bolduc O.R., Pelletier J.N., Masson J.F. (2010) SPR Biosensing in Crude Serum Using Ultralow Fouling Binary Patterned Peptide SAM. Anal Chem., 82 (9):3699-706. Doucet, N., and Pelletier, J. N. (2009) Gaining insight into enzyme function through correlation with protein motions. In: Protein Engineering Handbook. S. Lutz, and U. Bornscheuer, eds., Wiley-VCH, Weinheim pp187-211.