Bar-Peled, Maor - The University of Georgia - Department of Biochemistry & Molecular Biology

个人简介

Pectic and hemicellulosic polysaccharides are assembled in the Golgi apparatus by glycosyl transferases that catalyze the transfer of a glycosyl residue from a nucleotide sugar to a acceptor molecule. These polysaccharides are transported in vesicles that fuse with the plasma membrane and then released and integrated into the pre-existing wall. In contrast, cellulose is synthesized by protein complexes located in the plasma membrane. Cell wall polysaccharides may be modified as the cell grows and develops.

研究领域

Wall biosynthesis research at the CCRC is directed towards understanding at a molecular level the factors that control the biosynthesis of pectins and hemicelluloses. These studies involve: 1. Characterization of the galacturonosyl transferase(s) involved in the synthesis of homogalacturonan 2. Characterization of the enzymes involved in the formation of UDP-D-xylose, UDP-L-rhamnose, and UDP-D-apiose 3. The development of assays for glycosyl transferases and enzyme that interconvert nucleotide sugars 4. Determination of the topology of Golgi-localized enzymes 5. Characterization of the genes that encode for enzymes involved in wall biosynthesis

近期论文

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Bar-Peled et al (1991) UDP-rhamnose:flavone-7-O-glucoside-2"-O-rhamnosyltransferase. Purification and characterization of an enzyme catalyzing the production of bitter compounds in citrus. J. Biol. Chem. 266, 20953-20959. Goubet and Mohnen (1999) Subcellular localization and topology of homogalacturonan-methyltransferase in suspension-cultured Nicotiana tabacum cells. Planta, 209, 112-117. Bar-Peled et al (2001) Functional cloning and characterization of a UDP-glucuronic acid decarboxylase: The pathogenic fungfus Cryptococcus neoformans elucidates UDP-xylose synthesis. Proc. Nat. Acad Sci USA. 98, 12003-12008. Ridley et al (2001) Pectins: Structure, biosynthesis, and oligogalacturonide-related signaling. Phytochemistry, 57, 929-967. Tanner (2001) Sugar nucleotide-modifying enzymes. Curr Org Chem., 5, 169-192. Sterling et al (2001) The catalytic site of the pectin biosynthetic enzyme α-1,4-galacturonosyltransferase is located in the lumen of the Golgi. Plant Physiol., 127, 360-371. Harper and Bar-Peled (2002) Biosynthesis of UDP-xylose. Cloning and characterization of a novel Arabidopsis gene family, UXS, encoding soluble and putative membrane-bound UDP-glucuronic acid decarboxylase isoforms. Plant Physiol., 130, 2188-2198. Mohnen (2002) Biosynthesis of pectins. In Pectins and their Manipulation (Seymour GB and Knox JP eds.) Oxford:Blackwell Publishing and CRC Press, pp 52-98. Guillaumie et al (2003) Solid-supported enzymatic synthesis of pectic oligogalacturonides and their analysis by MALDI-TOF mass spectrometry. Carbohydr. Res., 338, 1951-1960. Watt et al (2004) A bifunctional 3,5-epimerase/4-keto reductase for nucleotide-rhamnose synthesis in Arabidopsis. Plant Physiol., 134, 1337-1346. Frydman et al (2004) Citrus fruit bitter flavors: isolation and functional characterization of the gene Cm1,2RhaT encoding a 1,2 rhamnosyltransferase, a key enzyme in the biosynthesis of the bitter flavonoids of citrus. Plant J. 40, 88-98. Gu and Bar-Peled (2004) The biosynthesis of UDP-galacturonic acid in plants. Functional cloning and characterization of Arabidopsis UDP-D-glucuronic acid 4-epimerase. Plant Physiol., 136, 4256-4264 Sterling et al (2005) Development of a filter assay for measuring homogalacturonan:α-(1,4)-Galacturonosyltransferase activity. Anal. Biochem., 343, 231-236. Sterling et al (2006) Functional identification of an Arabidopsis pectin biosynthetic homogalaturonan galacturonosyltransferase. Proc Nat Acad Sci (USA), 103, 5236-5241. Bacic (2006) Breaking an impasse in pectin biosynthesis. Proc Natl Acad Sci (USA), 103, 5639-5640.