研究领域

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Our research concerns the mechanism of Ty1 element retrotransposition and copy number control in the budding yeast Saccharomyces. Ty elements comprise five related families of long terminal repeat (LTR) retrotransposons that transpose through an RNA intermediate. The Ty life cycle resembles that of retroviruses except Ty transposition is not infectious. Ty element GAG and POL genes encode the structural and enzymatic proteins required for retrotransposition. The retrotransposon is transcribed from LTR to LTR to form a genome-length RNA, which is the template for reverse transcription by Ty reverse transcriptase and for protein synthesis. Ty RNA and proteins accumulate in cytoplasmic foci termed retrosomes, which mark the sites for assembly of virus-like particles (VLPs). Reverse transcription takes place within VLPs following protein maturation by an element-encoded protease. A preintegration complex minimally containing Ty cDNA and integrase transits the nuclear membrane and mediates integration at preferred chromosomal locations.