研究领域

Our current and future research is focused on the development of new generation instrumentation and methodologies for biomedical and behavioral research. In particular, our group is interested in the characterization of the chemical environment at the single cell and sub-cellular level of model cell systems and tissue sections using a "molecular microscope". There are two main challenges in the molecular characterization of native surfaces: the quantity of sample available for analysis and the dynamic mass range. The long-term goal of our work is to develop nanometer probes for mass spectrometry based imaging for the study at the single cell and sub-cellular level of "native state" biological surfaces with: i) enhanced lateral resolution, ii) enhanced emission of molecular ions and iii) fast gas-phase, post-ionization separation techniques. This integrated approach involves the instrument development and optimization of sample-friendly conditions for the generation of the molecular ions of interest, the design and generation of MS imaging calibration procedures and standards, the incorporation of high-throughput post-ionization separation and fragmentation techniques, the analysis of the gas-phase conformational space of molecular ions, and the characterization of the chemical environment at the single cell and sub-cellular level of model biological systems.

Analytical Chemistry Mass Spectrometry Ion Mobility Spectrometry Cluster-SIMS Surface Science Physical Chemistry Bioanalytical Chemistry Biophysical Chemistry

近期论文

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77. P. Benigni, K. Sandoval, C. J. Thompson, M. E. Ridgeway, M. A. Park, P. Gardinali, F. Fernandez-Lima*,” Analysis of Photoirradiated Water Accommodated Fractions of Crude 2 Oils Using Tandem TIMS and FT-ICR MS”, Environ. Sci. Technol. 2017. IF = 5.4 PDF 76. P. Benigni, R. Marin, K. Sandoval, P. Gardinali, F. Fernandez-Lima, “Chemical Analysis of Water-Accommodated Fractions of Crude Oil Spilss using TIMS-FT-ICR MS”. J. Vis. Exp. (121), e55352, doi:10.3791/55352, 2017. IF = 1.3 Website 75.P. Benigni, C. Bravo, J. M. E. Quirke, J. D. DeBord, A. M. Mebel, F. Fernandez-Lima, “Analysis of geologically relevant metal-porphyrins using Trapped Ion Mobility Spectrometry-Mass Spectrometry and theoretical calculations”. Energy and Fuels, 30 (12), pp 10341–10347, 2016. IF = 2.8 PDF 74. A. Garabedian, D. Butcher, J. L. Lippens, J. Miksovska, P. Chapagain, D. Fabris, M. E. Ridgeway, M. A. Park, F. Fernandez-Lima*, “Structures of kinetically trapped i-motif DNA intermediates”. Phys. Chem. Chem. Phys. 18 (38), 26691-26702, 2016. IF = 4.5 PDF 73. K. Adams, J. D. DeBord, F. Fernandez-Lima*, “Lipid specific molecular ion emission as a function of the primary ion characteristics in TOF-SIMS”. J. Vac. Sci. Technol. B. 34, 051804, 2016. IF = 1.4. PDF 72. Q. P. Vanbellingen, A. Castellanos, M. Rodriguez-Silva, I. Paudel, J. W. Chambers, F. Fernandez-Lima*, “Analysis of chemotherapeutic drug delivery at the single cell level using 3D-MSI-TOF-SIMS”, J. Am. Soc. Mass Spectrom. (in press), 2016. IF = 3.0 PDF 71. P. Benigni, F. Fernandez-Lima*, "Oversampling Selective Accumulation Trapped Ion Mobility Spectrometry coupled to FT-ICR MS: Fundamentals and Applications", Anal .Chem. 88 (14), 7404–7412, 2016. IF = 5.9. PDF 70. C. E Ramirez, M. Nouzova, P. Benigni, J M. E Quirke, F. G Noriega, F. Fernandez-Lima*, "Fast, ultra-trace detection of Juvenile Hormone III from mosquitoes using mass spectrometry", Talanta 159, 371-378, 2016. IF = 4.0. PDF 69. F. Fernandez Lima*, “Trapped Ion Mobility Spectrometry: Past, Present and Future Trends”, Editorial, Int. J. Ion Mobil. Spectrom. 19, 65-67, 2016. IF=1.1; PDF 68. P. Benigni, R. Marin, J. C. Molano-Arevalo, A. Garabedian, J. J. Wolff, M. E. Ridgeway, M. A. Park, F. Fernandez-Lima*, "Towards the analysis of high molecular weight proteins and protein complexes using TIMS-MS", Int. J. Ion Mobil. Spectrom., 19, 95-104, 2016. IF = 1.1 PDF 67. A. Castellanos, S. Bell, F. Fernandez-Lima*, “Characterization of Firearms Discharge Residue Recovered from Skin Swabs using Sub-micrometric Mass Spectrometry Imaging”, Anal. Methods. 8, 4300-4305, 2016. IF = 1.8 PDF 66. W. G. Gonzalez, V. Ramos, M. Diaz, A. Garabedian, J. C. Molano-Arevalo, F. Fernandez-Lima, J. Miksovska*, “Characterization of the photophysical, thermodynamic and structural properties of the Terbium(III)-DREAM complex”. Biochemistry, 55(12), 1873-1886, 2016. IF = 3.0 PDF 65. K. J. Adams, D. Montero, D. Aga, F. Fernandez-Lima*, “Isomer Separation of Polybrominated Diphenyl Ether Metabolites using nanoESI-TIMS-MS”. Int. J. Ion Mobil. Spectrom., 19, 69-76, 2016 2016. IF = 1.1 PDF 64. P. Benigni, J. Daniel DeBord, C. Thompson, P. Gardinali, F. Fernandez-Lima*, "Increasing Polyaromatic Hydrocarbon (PAH) Molecular Coverage during Fossil Oil Analysis by Combining Gas Chromatography and Atmospheric-Pressure Laser Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS)". Energy and Fuels, 30 (1), 196–203, 2016. IF = 2.8 PDF 63 A. McKenzie, J. D. DeBord, M. E. Ridgeway, M. A. Park, G. Eiceman, F. Fernandez-Lima*, “Lifetimes and Stabilities of familiar explosives molecular adduct complexes during ion mobility measurements”. Analyst, 140, 5692-5699, 2015. IF = 4.1 PDF 62. P. Benigni, R. Marin, F. Fernandez-Lima*. “Towards unsupervised theoretical PAH structural assignment from SA-TIMS –FTMS data” Int. J. Ion Mob. Spectrom., 18:151–157, 2015. IF = 1.1 PDF 61. L. Frost, M. A. M. Baez, C. Harrilal, A. Garabedian, F. Fernandez-Lima, F. Leng* “The dimerization state of the mammalian high mobility group protein AT-hook 2 (HMGA2).” PLoS One, 10 (6), e0130478, 2015. IF = 4.0 PDF 60. E. R. Schenk, R. Almeida, J. Miksovska, M. E. Ridgeway, M. A. Park, F. Fernandez-Lima*, “Kinetic intermediates of holo- and apo- myoglobin studied using HDX-TIMS-MS and molecular dynamic simulations.” J. Am. Soc. Mass Spectrom. 26 (4), 555-563, 2015. IF = 3.0 PDF 59. P. Benigni, C. J. Thompson, M. E. Ridgeway, M. A. Park, F. Fernandez-Lima*, "Targeted high resolution ion mobility separation coupled to ultra-high resolution mass spectrometry of endocrine disruptors in complex mixtures". Anal. Chem. 87 (8), 4321–4325, 2015. IF = 5.8 PDF 58. E. R. Schenk, F. Nau, F. Fernandez-Lima*. “Theoretical predictor for candidate structure assignment from IMS data of biomolecule-related conformational space”. Int. J. Ion Mobility Spectrom., 18(1-2) 23-29, 2015. IF = 1.1 PDF 57. E. R. Schenk, F. Nau, C. J. Thompson, Y.C. Tse-Dinh, F. Fernandez-Lima*, ”Changes in lipid distribution in E. coli strains in response to norfloxacin”, J. Mass Spectrom. 50, 88–94, 2015. IF = 3.0 PDF