Barbieri, Alejandro - Florida International University

研究领域

Our research group is interested the following two areas of research. Endocytosis and Signal transduction How the small GTP Rab5, is linked to the oncogene Ras, tyrosine receptor kinases and other intracellular signaling molecules. This pathway may provide a model for understanding how receptor ligand interaction regulates intracellular trafficking while simultaneously generating proliferative signals in normal and disease cells. Phagocytosis and signal transduction Internalization of pathogens into the phagocytic vacuoles is a critical component of the innate immune response. Following formation, the phagocytic vacuoles (i.e. phagosomes) interact with the endocytic compartment. This process (i.e. phagosome maturation) involves multiple fusion and fision events with the endomembrane system, which in turn, culminate with the formation of the phagolysosome. We are using the Fc receptor as model to determine the factors that initiate and coordinate the phagosome maturation process.

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M.A. Sosa, M.A. Barbieri and F. Bertini. Binding of β-galactosidase from rat epididymal fluid to the sperm surface by high-affinity sites different from phosphomannosyl receptors. J. Reprod. Fert. 93: 279 (1991). M.W. Fornes, M.A. Barbieri, M.A. Sosa and F. Bertini. First observations on enzymatic activity and protein content of vesicles separated from rat epididymal fluid. Andrologia 23: 347 (1991). M.A. Barbieri, E. Lammel, E.L. Isola and F. Bertini. Trypanosome cruzi: metacyclogenesis inhibition by mannose. Biochem. Biophys. Res. Commun. 187: 108 (1992). M.W. Fornes, M.A. Barbieri and M.H. Burgos. Sperm motility loss induced by gossypol: relation with OH scavengers, motile stimulator and malondialdehyde production. Biochem. Biophys. Res. Commun. 195: 1289 (1993). M.A. Barbieri, M.A. Sosa, R. Couso, L. Ielpi, S. Merello, C.E. Tonn and F. Bertini. High affinity sites for N-acetyl-β-D-glucosaminidase on the surface of rat epididymal spermatozoa. International J. Andrology 17: 43 (1994). M.W. Fornes, M.A. Barbieri and C. Cavicchia. Morphological and enzymatic study of membrane-bound vesicles from the lumen of rat epididymis. Androligia 27: 1 (1994). G. Li, M.A. Barbieri, M.I. Colombo and P.D. Stahl. Structural features of the GTP-binding defective rab5 mutants required for their inhibitory activity on the endocytosis. J. Biol. Chem. 269: 1463 (1994). G. Li, M.A. Barbieri and P.D. Stahl. Myristoylation cannot functionally replace the isoprenylation of rab5. Arch. Biochem. Biophys. 316: 529 (1994). M.A. Barbieri, M.I. Colombo, G. Li, L.S. Mayorga and P.D. Stahl. GTPases: Key regulatory components of the endocytic pathway. Trafficking of intracellular Membranes: From Molecular Sorting to Membrane Fusion. (M.C. Pedrosao De Lima, N. Düzgüeç and D. Hoekstra, eds). Springer-Verlag Berlin Heiderberg, Germany, NATO ASI Series H: Cell Biology. 91:223 (1994). M.A. Barbieri, G. Li, M.I. Colombo and P.D. Stahl. Rab5, an early acting endosomal GTPase, supports in vitro endosomal fusion without GTP hydrolysis. J. Biol. Chem. 269: 18720 (1994). G. Li, C. D'Souza-Schorey, M.A. Barbieri, R.L. Roberts, A. Klippel, L.T. Williams and P.D. Stahl. Evidence for phosphatidylinositol 3-kinase as a regulator of endocytosis via activation of rab5. Proc. Natl. Acad. Sci. U.S.A. 92: 10207 (1995). P. Grimalt, M.A. Barbieri, M.A. Sosa and F. Bertini. Organ specific binding system for β-galactosidase in the male reproductive tract. International J. Andrology: 18: 243 (1995). M.A. Barbieri, M.A. Sosa, L.S. Mayorga, P. Grimalt and F. Bertini. Phosphomannosyl receptors on the surface of spermatozoa from the cauda epididymis of rat. International J. Andrology 18: 113 (1995). M.A. Barbieri, G. Li, L.S. Mayorga and P.Stahl. Characterization of rab5-GTP stimulated endosome fusion. Archives of Biochem. Biophys. 326: 64 (1996). C. Alvarez-Dominguez, M.A. Barbieri, W. Beron, A, Wandinger-Ness and P.D.Stahl. Phagocytosed live Listeria monocytogenes influences rab5 regulated in vitro phagosome-endosome fusion. J. Biol. Chem. 271: 13834 (1996). M.A. Barbieri, M.L.Veisaga, F. Paolicchi, M.W.Fornes, M.A. Sosa, L.S. Mayorga, E. Bustos-Obregon and F. Bertini. Affinty sites for β-glucuronidase on the surface of human spermatozoa. Andrologia. 28 :148 (1996). E. Lammel, M.A. Barbieri, F. Bertini and E.D Isola. Trypanosoma cruzi: involvement of intracellular calcium in multiplication and differentiation. Experimental Parasitology. 85: 240 (1996). M.W. Fornes, M.A. Barbieri and F.Bertini. Electrophoretic analysis of protein selectively extracted with chaotropic agents from membranes of rat sperm. BioCell. 20: 111 (1996). M.A. Sosa, M.A.Barbieri and F.Bertini. Purification and characterization of β-galactosidase from rat epididymal fluid. Andrologia. 28: 71 (1996). M.A. Barbieri. A. Mukhopadhyay, R.L. Roberts, and P.D. Stahl. Rab5 in endosome fusion. Biocell. 20: 331 (1996). A. Mukhopadhyay, M.A. Barbieri, K. Funato, R. Roberts and P.D. Stahl. Sequential actions of rab5 and rab7 regulate endocytosis in the Xenopus oocyte. J. Cell Biol. 136: 1227 (1997). G. Li, C. D’Souza-Schorey, M.A. Barbieri, J.A. Cooper and P.D.Stahl . Uncoupling of membrane ruffling and pinocytosis during ras signal transduction. J Biol Chem. 272:10337 (1997). M.A. Barbieri, S. Hoffermberg, A. Mukhopadhyay, B.F.Dickey and P.D.Stahl. Evidence for a symmetrical requirement for Rab5-GTP in in vitro endosome-endosome fusion. J Biol Chem. 273:25850 (1998). M.A. Barbieri, A.D. Khon, R.A. Roth, and P.D.Stahl. Protein kinase B/akt and rab5 mediate Ras activation of endocytosis. J Biol Chem. 273:19367 (1998). A. Aballay, M.A. Barbieri, M.I. Colombo, G.N. Arenas, P.D. Stahl, and L.S Mayorga. A phorbol ester-binding protein is required downstream of Rab5 in endosome fusion. FEBS Lett. 441:373 (1998).