研究领域
Magnetic Resonance Microscopy
Neurodegenerative Diseases
Muscular Degeneration
Bioengineered Constructs & Materials
High Field MRI Contrast Mechanisms & Agents
Single Cell Analysis – Diffusion, Spectroscopy & Osmoregulation
Magnetic Resonance Imaging and Spectroscopy are extremely powerful analytical methods that provide not only high information content but are non-invasive and non-destructive to the sample under analysis.
My research laboratory is focused on the development of high resolution techniques to investigate the biophysical origins of MR signals under a variety of perturbations. We utilize high magnetic fields to achieve high sensitivity and spatial/ spectral resolution on specimen ranging from single isolated neurons to fixed neurological tissues (brains and spinal cords) to in vivo animal models. Our close affiliation with the National High Magnetic Field Laboratory provides access to the highest magnetic fields in the world, including the one-of-a-kind ultrawide bore 21.1-T system for MR imaging and spectroscopy.
In particular, we employ high fields MR microscopy to examine neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer's disease and stroke. In collaboration with neurologists, clinicians and biologists, we make use of genetic, toxic and surgical models to identify biomarkers of disease progression that might have diagnostic or therapeutic value clinically. Furthermore, we evaluate potential treatments (e.g. stem cell and drug therapy) with MR techniques in order to judge their efficacy in restoring normal cellular function.
MR microscopy also lends itself to the study of biomaterials and bioartificial devices. Because it is non-destructive, MR techniques can be utilized to analyze engineered constructs during their in vitro development to map growth patterns and cell-material interactions. Following implantation, constructs can be monitored in vivo to assess immunoresponse, mechanical integrity and integration into existing bioprocesses. Throughout this continuum, MR microscopy provides information about the biomaterial substrate, cellular component and functionality of these engineered constructs.
To make the most of high field MR techniques in these evaluations, my laboratory is actively involved in MR sequence development, modeling of cellular compartmentalization & function and Radio Frequency coil design. In addition, we are interrogating new and emerging contrast mechanisms at high field. These efforts include endogenous (e.g. magnetic susceptibility and dipolar fields) and exogenous (e.g. nanoparticle agents and current density imaging) contrasts that may provide new insights into the biophysical changes that occur during pathology or regeneration.
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A.G.Webb and S.C.Grant, J. Magn. Reson. Ser. B, 113: 83-7 (1996). "Signal-to-noise and magnetic susceptibility trade-offs in solenoidal microcoils for NMR."
S.C.Grant, H.D.Plant, S.Gibbs, N.R.Aiken, A.G.Webb, T.H.Mareci and S.J.Blackband, Magn. Reson. Med., 44: 19-22 (2000). "NMR Spectroscopy of Single Neurons."
S.C.Grant, L.A.Murphy, R.L.Magin and G.Friedman, IEEE Trans. Magn., 37(4): 2989-98 (2001). "Analysis of Multilayer RF Microcoils for NMR Spectroscopy."
S.C.Grant, D.L.Buckley, S.Gibbs, A.G. Webb, S.J.Blackband, Magn. Reson. Med., 46(6): 1007-12 (2001). "MR Microscopy of Multiple Component Water Diffusion in Isolated Single Neurons."
B.Beck, H.D.Plant, S.C.Grant, P.E.Thelwall, X.Silver, T.H.Mareci, H.Benveniste, M.Smith, C.Collins, S.Crozier, S.J.Blackband, MAGMA, 13(3): 152-7 (2002). "Progress in High Field MRI at the University of Florida."
P.E.Thelwall, S.C.Grant, S.J.Blackband, Magn. Reson. Med., 48: 649-57 (2002). "Human Erythrocyte Ghosts: Exploring the origins of multiexponential water diffusion in a model biological tissue with magnetic resonance."
N.E.Simpson, S.C.Grant, S.J.Blackband and I.Constantinidis, Biomaterials, 24: 4941-4948 (2003). "NMR Properties of Alginate Microbeads."
J.M.B.Wilson, M.S.Petrik, S.C.Grant, S.J.Blackband, J.Lai and C.A.Shaw, NeuroImage, 33: 336-343 (2004). "Quantitative Measurement of Neurodegeneration in an ALS-PDC Model Using MR Microscopy."
S.C.Grant, S.Celper, I.Gauffin-Holmberg, N.E.Simpson, S.J.Blackband and I.Constantinidis, J. Mater. Sci.: Mater. Med., 16: 511-14 (2005). "Alginate Assessment by NMR Microscopy."
R.Sadleir, S.C.Grant, S.U.Zhang, B.I.Lee, H.C.Pyo, S.H.Oh, C.Park, E.J.Woo, S.Y.Lee, O.Kwon and J.K.Seo, Physiol. Meas., 26: 875–84 (2005). "Noise analysis in magnetic resonance electrical impedance tomography at 3 and 11 T field strengths."
R.Fu, W.W.Brey, K.Shetty, P.Gor'kov, S.Saha, J.R.Long, S.C.Grant, E.Y.Chekmenev, J.Hu, Z.Gan, M.Sharma, F.Zhang, T.M.Logan, R.Bruschweller, A.Edison, A.Blue, I.R.Dixon, W.D.Markiewicz and T.A.Cross, J. Magn. Reson., 177(1): 1-8 (2005). "Ultra-wide bore 900MHz high-resolution NMR at the National High Magnetic Field Laboratory."
Y.Ma, P.R.Hof, S.C.Grant, S.J.Blackband, R.Bennett, L.Slatest, M.D.McGuigan and H.Benveniste. Neuroscience, 135(4): 1203-15 (2005). "A three-dimensional digital atlas database of the adult C57BL/6J mouse brain by magnetic resonance microscopy."
J.P.Marques, S.C.Grant, S.J.Blackband and R.W.Bowtell, J. Chem. Phys., 123(16): 164311 (2005). "Intermolecular Multiple Quantum Coherences at High Magnetic Field: the Non-Linear Regime."
I.Constantinidis, N.E.Simpson, S.C.Grant, S.J.Blackband, R.C.Long Jr, A.Sambanis, Adv. Exp. Med. Biol., 585: 261-76 (2006). "Non-invasive monitoring of tissue engineered pancreatic constructs by NMR techniques."
N.E.Simpson, S.C.Grant, L.Gustavssond, V.M. Peltonen, S.J.Blackband and I.Constantinidis. Biomaterials, 27: 2577-2586 (2006). "Biochemical consequences of alginate encapsulation: A NMR study of insulin-secreting cells."
R.Sadleir, S.C.Grant, S.U.Zhang, S.H.Oh, B.I.Lee and E.J.Lee, Physiol. Meas., 27: S261-S270 (2006). "High Field MREIT: Setup and tissue phantom imaging at 11 T."
I.Constantinidis*, S.C.Grant*, S.Celper, I.Gauffin-Holmberg, K.Agering, J.A. Oca-Cossio, J.D.Bui, J.Flint, C.Hamaty, N.E. Simpson and S.J. Blackband, Biomaterials, 28(15): 2438-45 (2007). "Non-invasive evaluation of alginate/poly-L-lysine/alginate microcapsules by Magnetic Resonance Microscopy." * co-first authors
H.Benveniste, Y.Ma, J.Dhawan, A.Gifford, S.D.Smith, I.Feinstein, C.Du, S.C.Grant and P.R.Hof, NY Acad. Sci., 1097: 12-29 (2007). "Anatomical and Functional Phenotyping of Mice Models of Alzheimer's Disease by MR Microscopy."
M.S.Petrik, J.M.B.Wilson, S.C.Grant, S.J.Blackband, R.C.Tabata, X.Shan, C.Krieger and C.A.Shaw, NeuroMolecular Med., 9(3): 216-29 (2007). "Magnetic resonance microscopy and immunohistochemistry of the central nervous system of the mutant SOD murine model of ALS."
Constantinidis, I., Grant, S.C., Simpson, N.E., Oca-Cossio, J.A., Sweeney, C.A., Mao, H., Blackband, S.J., and Sambanis, A., "Use of Magnetic Nanoparticles to Monitor Alginate-Encapsulated βTC-tet Cells," Magn Reson Med, 61(2): 282-90 (2009).
Schweitzer, K.J., Foroutan, P., Dickson, D.W., Broderick, D.F., Klose, U., Berg, D., Wszolek, Z.K., & Grant, S.C. A novel approach to dementia: High resolution 1H MRI of the human hippocampus performed at 21.1 T. Neurology, 74(20), 1654-1654. (2010).
Schepkin, V.D., Brey, W.W., Gor'kov, P.L., & Grant, S.C. Initial in vivo rodent sodium and proton MR imaging at 21.1 T. Magnetic Resonance Imaging, 28(3), 400-407. (2010).
Sadleir, R.J., Grant, S.C., & Woo, E. J. Can high-field MREIT be used to directly detect neural activity? Theoretical considerations. NeuroImage, 52(1), 205-216. (2010).
Rosenberg, J. T., Kogot, J. M., Lovingood, D. D., Strouse, G. F., & Grant, S. C. Intracellular Bimodal Nanoparticles Based on Quantum Dots for High-Field MRI at 21.1 T. Magnetic Resonance in Medicine, 64(3), 871-882. (2010).
Fujioka, S., Murray, M. E., Foroutan, P., Schweitzer, K. J., Dickson, D. W., Grant, S. C., & Wszolek, Z. K. Magnetic resonance imaging with 21.1T and pathological correlations – diffuse Lewy body disease. Rinsho Shinkeigaku, 51(8), 603-607. (2011).
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