COLE, AMY - University of Central Florida - Division of Molecular Microbiology

研究领域

Our research interests include the study of several factors that modulate inflammatory pathways in the female genital tract (FGT). We have identified cationic antimicrobial peptides that contribute to the anti-viral capabilities of vaginal fluid. Former student projects have also identified pro-HIV molecules produced by endocervical cells exposed to bacterial vaginosis-associated bacteria. Current studies include fractionation of these co-culture fluids in order to identify specific proteins effecting epithelial barrier function, NFkB activation, and chemotaxis of HIV target cells. We collaborate with faculty from UCF and abroad in the pursuit of therapeutics that provide potent anti-viral activity without disturbing the dynamic environment of the FGT mucosa. We are also a GCLP-certified specimen processing lab for the HIV Vaccine Trials Network, and perform clinical processing for several Orlando-area clinical trials

近期论文

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Venkataraman, N., Cole, A.L.*, Svoboda, P., Pohl, J, Cole, A.M. Cationic polypeptides are required for anti-HIV-1 activity of human vaginal fluid. J. Immunol. 175(11): 7560-7, 2005 Cole, A.L., Yang, O.O., Warren, A.D., Waring, A.J., Lehrer, R.I., Cole, A.M. HIV-1 adapts to a retrocyclin with cationic amino acid substitutions that reduce fusion efficiency of gp41. J. Immunol. 176(11): 6900-5, 2006 Cole, A.L., Herasimtschuk, A., Gupta, P., Waring, A.J., Lehrer, R.I., and Cole, A.M. The retrocyclin analogue RC-101 prevents human immunodeficiency virus type 1 infection of a model human cervicovaginal tissue construct. Immunology 121(1): 140-5, 2007 Keller M.J., Guzman, E., Hazrati, E., Kasowitz, A., Cheshenko, N., Wallenstein, S., Cole, A.L., Cole, A.M., Profy, A.T., Wira, C.R., Hogarty, K., Herold, B.C. PRO 2000 elicits a decline in genital tract immune mediators without compromising intrinsic antimicrobial activity. AIDS. 21(4): 467-476, 2007 Fuhrman, C.A., Warren, A.D., Waring, A.J., Dutz, S.M., Sharma, S., Lehrer, R.I., Cole, A.L., and Cole, A.M. Retrocyclin (RC)-101 overcomes cationic mutations on the heptad repeat 2 region of HIV-1 gp41. FEBS J. 274(24): 6477-87, 2007 Cole, A.M., Cole, A.L. Antimicrobial polypeptides are key anti-HIV-1 effector molecules of cervicovaginal host defense. Am. J. Reprod. Immunol. 59(1): 27-34, 2008 Martellini, J.A., Cole, A.L., Venkataraman, N., Quinn, G.A., Svoboda, P., Gangrade, B.K., Pohl, J., Sorensen, O.E., Cole, A.M. Cationic polypeptides contribute to the anti-HIV-1 activity of human seminal plasma. FASEB J. 23(10): 3609-18, 2009 Cole, A.M., Patton, D.L., Rohan, L.C., Cole, A.L., Cosgrove-Sweeney, Y., Rogers, N.A., Ratner, D., Sassi, A., Tarwater, P., Ramratnam, B., Ruchala, P., Lehrer, R.I., Waring, A.J., and Gupta, P. The formulated microbicide RC-101 was safe and antivirally active following intravaginal application in pigtailed macaques. PLoS ONE 5(11): e15111, 2010. Sassi, A.B., Cost, M.R., Cole, A.L., Cole, A.M., Patton, D.L., Gupta, P., and Rohan, L.C. Formulation development of Retrocyclin-1 (RC-101) as an anti-HIV vaginal microbicide product. Antimicrob Agents and Chemother 55(5): 2282-9, 2011 Martellini, J.A., Cole, A.L., Svoboda, P., Stuchlik, O., Chen, L.M., Chai, K.X., Gangrade, B.K., Sorensen, O.E., Pohl, J., and Cole, A.M. HIV-1 enhancing effect of prostatic acid phosphatase peptides is reduced in human seminal plasma. PLoS ONE 6(1): e16285, 2011. Ruchala, P., Cho, S., Cole, A.L., Carpenter, C., Jung, C-L., Luong, H., Micewicz, E.D., Waring, A.J., Cole, A.M., Herold, B.C., Lehrer, R.I. Simplified theta-defensins: search for new antivirals. Int. J. Peptide Res. and Ther. Published online Sept 23, 2011 Levinson, P., Choi, R.Y., Cole, A.L., Hirbod, T., Rhedin, S., Payne, B., Guthrie, B.L., Bosire, R., Cole, A.M., Farquhar, C., Broliden, K. HIV-Neutralizing Activity of Cationic Polypeptides in Cervicovaginal Secretions of Women in HIV-Serodiscordant Relationships. PLoS One 2012; 7(2):e31996. Epub 2012 Feb 28 Eade, C.R., Diaz, C., Wood, M.P., Anastos, K., Patterson, B.K., Gupta, P., Cole, A.L., Cole, A.M. Identification and characterization of bacterial vaginosis-associated pathogens using a comprehensive cervical-vaginal epithelial coculture assay. PLOS One. 7(11): 250106 Epub 2012 Nov15 Eade, C.R., Cole, A.L.*, Diaz, C., Rohan, L.C., Parniak, M.A., Marx, P., Tarwater, P.M., Gupta, P., Cole, A.M. The anti-HIV microbicide candidate RC-101 inhibits pathogenic vaginal bacteria without harming endogenous flora or mucosa. Amer J Rep Immunol. 69(2): 150-8, 2013 Wood, M.P., Cole, A.L., Ruchala, P., Waring, A.J., Rohan, L.C., Marx, P., Tarwater, P.M., Gupta, P., Cole, A.M. A compensatory mutation provides resistance to disparate HIV fusion inhibitor peptides and enhances membrane fusion. PLOS One. 8(2):e55478 Epub 2013 Feb5 Wood. M.P., Cole, A.L., Eade, C.R., Chen, LM, Chai, K.X., Cole, A.M. The HIV-1 gp41 ectodomain is cleaved by matriptase to produce a chemotactic peptide that acts through FPR2. Accepted to Immunology, March 6, 2014