黄硕 - 南京大学 - 化学化工学院

个人简介

2006年于南京大学物理系获得物理学学士学位；2011年于美国亚利桑那州立大学获得生物物理学博士学位；导师是Stuart Lindsay教授；2011年至2015年在英国牛津大学从事博士后研究工作，导师是Hagan Bayley教授。 2015年7月应聘南京大学化学化工学院教授。现从事主要科研方向为具有仿生学概念的单分子纳米孔生物传感器的新仪器、新技术的开发以及基于纳米孔的单分子显微成像技术。其中在纳米孔单分子成像方面具有原创性技术，具有国际前沿研发水平。目前已于国际权威期刊Nature Methods，Nature Nanotechnology，Nature Communications，Journal of the American Chemical Society, Science Advances，Angewandte Chemie International Edition, ACS Nano, Nano Letters等期刊发表论文若干。其中以第一作者发表Nature Nanotechnology 2篇，并入选2010年12月刊封面；以唯一通讯作者发表Nature Methods 1篇, Nature Nanotechnology 1篇，并入选2022年9月刊封面。已授权国际专利两项、国内专利1项，申请PCT专利若干项。

研究领域

单分子生物物理，基于纳米孔器件的单分子生物传感器，具有临床检测功能的单分子医疗诊断

近期论文

查看导师新发文章（温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

Wang, K. #; Zhang, S. #; Zhou, X. #; Yang, X.; Li, X.; Wang, Y.; Fan, P.; Xiao, Y.; Sun, W.; Zhang, P.; Li, W.; Huang, S. *; Unambiguous discrimination of all 20 proteinogenic amino acids and their modifications by nanopore.Nature Methods,2024,21 (1), 92-101. Zhang, S.#; Cao, Z.#; Fan, P.#; Sun, W.; Xiao, Y.; Zhang, P.; Wang, Y. *; Huang, S.*, Discrimination of Disaccharide Isomers of Different Glycosidic Linkages Using a Modified MspA Nanopore.Angewandte Chemie International Edition,2024,63 (8), e202316766. Fan, P.#; Cao, Z.#; Zhang, S.#; Wang, Y.; Xiao, Y.; Jia, W.; Zhang, P.; Huang, S.*, Nanopore analysis of cis-diols in fruits.Nature Communications,2024,15 (1), 1969. Fan, P.#; Zhang, S.#; Wang, Y.; Li, T.; Zhang, H.; Zhang, P.; Huang, S.*, Nanopore analysis of salvianolic acids in herbal medicines.Nature Communications,2024,15 (1), 1970. Wang, K. #; Yang, X. #; Xiao, Y.; Cao, Z.; Zhang, S.; Zhang, P.; Huang, S.*, Simultaneous Identification of Major Thyroid Hormones by a Nickel Immobilized Biological Nanopore.Nano Letters,2024,24(1), 305–311 Li, X.; Wang, Y.; Zhang, S.; Zhang, P.; Huang, S.*, Nanopore Identification of N-Acetylation by Hydroxylamine Deacetylation (NINAHD)ACS Sensors,2024,https://doi.org/10.1021/acssensors.3c02350 Yang, J. #; Wang, K. #; Zhang, S.; Zheng, X.; Cui, T.; Yang, X.; Liu, Y.; Lu, D.; Wang, Y.; Tian, X.; Zhang, P.; Huang, S. *; Xie, R. *, Site-Specific Introduction of Bioorthogonal Handles to Nanopores by Genetic Code Expansion.Angewandte Chemie International Edition,2023, 62(21)e202216115 Du, X.#; Zhang, S.#; Wang, L.; Wang, Y.; Fan, P.;Jia, W.; Zhang, P.; Huang, S.* Single Molecule Interconversion between Chiral Configurations of Boronate Esters Observed in a Nanoreactor.ACS Nano,2023,17(3), 2881–2892 Jia, W.; Ouyang, Y.; Zhang, S.; Du, X.; Zhang, P.; Huang, S., Nanopore Signatures of Nucleoside Drugs.Nano Letters,2023,23(20), 9437–9444 Wang, K. #; Yang, X. #; Zhang, S.; Zhang, P.; Huang, S.*, Nanopore Discrimination of Nucleotide Sugars.Nano Letters,2023,23(18), 8620–8627 Wang, Y.#; Zhang, S. #; Jia, W. #; Fan, P.; Wang, L.; Li, X.; Chen, J.; Cao, Z.; Du, X.; Liu, Y.; Wang, K.; Hu, C.; Zhang, J.; Hu, J., Zhang, P.; Chen, H.-Y.; Huang, S.* Identification of nucleoside monophosphates and their epigenetic modifications using an engineered nanopore.Nature Nanotechnology,2022,17 (9), 976-983. Liu, Y.#; Zhang, S.#; Wang, Y.; Wang, L.; Cao, Z.; Sun, W.; Fan, P.; Zhang, P.; Chen, H.-Y.; Huang, S.*, Nanopore identification of alditol epimers and their application in rapid analysis of alditol-containing drinks and healthcare products.Journal of the American Chemical Society,2022,144(30), 13717–13728 Liu, Y.; Wang, K.; Wang, Y.; Yan, S.; Du, X.; Zhang, P.; Chen, H.-Y.; Huang, S.* Machine Learning assisted simultaneous structural profiling of differently charged proteins in a Mycobacterium smegmatis porin A (MspA) electroosmotic trap.Journal of the American Chemical Society,2022,144(2), 757–768 Zhang, S.#; Cao, Z.#; Fan, P.#; Wang, Y.; Jia, W.; Wang, L.; Wang, K.; Liu, Y.; Du, X.; Hu, C.; Zhang, P.; Chen, H.-Y.; Huang, S.*A Nanopore-Based Saccharide Sensor.Angewandte Chemie International Edition,2022,61(33): e202203769 Yan, S.#; Wang, L.#; Wang, Y.; Cao, Z.; Zhang, S.; Du, X.; Fan, P.; Zhang, P.; Chen, H.-Y.; Huang, S.* , Non-binary Encoded Nucleic Acid Barcodes Directly Readable by a Nanopore.Angewandte Chemie International Edition,2022,61 (20), e202116482. Wang, Y.#; Fan, P.#; Zhang, S.; Wang, L.; Li, X.; Jia, W.;Liu, Y.; Wang, K.; Du, X.; Zhang, P.; Huang, S.* Discrimination of ribonucleoside mono-, di-, and triphosphates using an engineered nanopore.ACS Nano,2022,16(12), 21356–21365 Jia, W.; Hu, C.; Wang, Y.; Liu, Y.; Wang, L.; Zhang, S.; Zhu, Q.; Gu, Y.; Zhang, P.; Ma, J.; Chen, H.-Y.; Huang, S*. Identification of Single-Molecule Catecholamine Enantiomers Using a Programmable NanoporeACS Nano,2022, 16(4): 6615-6624. Zhang, J. #; Wang, Y. #; Wang, Y.; Zhang, P.; Chen, H.-Y.; Huang, S.*, Discrimination between different DNA lesions by monitoring single molecule polymerase stalling kinetics during nanopore sequencing.Nano Letters,2022,22(13), 5561–5569 Jia, W.; Hu, C.; Wang, Y.; Zhang, P.; Chen, H.-Y.; Huang, S*. A Nanopore Based Molnupiravir Sensor,ACS Sensors,2022,7 (5), 1564-1571. Hu, C.; Jia, W.; Liu, Y.; Wang, Y.; Zhang, P.; Chen, H.-Y.; Huang, S.*. Single-Molecule Sensing of Acidic Catecholamine Metabolites Using a Programmable Nanopore,Chemistry - A European Journal,2022,28(44), e202201033. Guan, X.#; Wang, Y.#; Shao, W.; Li, Z.; Huang, S.*; Zhang, D*. S2Snet: deep learning for low molecular weight RNA identification with nanopore,Briefings in Bioinformatics,2022,23 (3), bbac098. Liu, Y.; Pan, T.; Wang, K.; Wang, Y.; Yan, S.; Wang, L.; Zhang, S.; Du, X.; Jia, W.; Zhang, P.; Chen, H.-Y.; Huang, S.*Allosteric Switching of Calmodulin in a Mycobacterium smegmatis porin A (MspA) Nanopore-Trap.Angewandte Chemie International Edition,2021,60(44), 23863–23870 Jia, W.; Hu, C.; Wang, Y.; Gu, Y.; Qian, G.; Du, X.; Wang, L.; Liu, Y.; Cao, J.; Zhang, S.; Yan, S.; Zhang, P.; Ma, J.; Chen, H.-Y.; Huang, S.*, Programmable Nano-Reactors for Stochastic Sensing.Nature Communications,2021,12(1), 5811 Wang, Y. Q.#; Guan, X. Y.#; Zhang, S. Y.; Liu , Y.; Wang, S.; Fan , P. P.; Du, X. Y.; Yan , S.; Zhang , P. K.; Chen, H.-Y.; Li , W. F.*; Zhang, D. Q.*; Huang, S.*,Structural-profiling of low molecular weight RNAs by nanopore trapping/translocation using Mycobacterium smegmatis porin A.Nature Communications,2021,12(1), 3368 Yan, S. H.; Zhang, J. Y.; Wang, Y.; Guo, W.M.; Zhang, S. Y.; Liu. Y.; Cao, J.; Wang, Y. Q.; Wang, L. Y.; Ma, F. B.; Zhang, P. K.; Chen, H. Y.; Huang, S.*，Single Molecule Ratcheting Motion of Peptides in a Mycobacterium smegmatis Porin A (MspA) Nanopore.Nano Letters,2021,21(15), 6703–6710 Yan, S. H.#; Wang, L. Y. #; Du, X. Y.; Zhang, S. Y.; Wang, S.; Cao, J.; Zhang, J. Y.; Jia, W. D.; Wang, Y. Q.; Zhang, P. K.; Chen, H.-Y.; Huang, S.*, Rapid and multiplex preparation of engineered Mycobacterium smegmatis porin A (MspA) nanopores for single molecule sensing and sequencing.Chemical Science,2021,12(27), 9339-9346 Du, X.#; Wang, Y.#; Zhang, S.; Fan, P.; Yan, S.; Zhang, P.; Chen, H.; Huang, S.*, Microscopic Screening of Cyclodextrin Channel Blockers by DiffusiOptoPhysiology.Analytical Chemistry,2021,93(42), 14161–14168 Cao, J.; Zhang, S. Y.; Zhang, J. Y.; Wang, S.; Jia, W. D.; Yan, S. H.; Wang, Y. Q.; Zhang, P. K.; Chen, H.-Y.;Huang, S.*, A Single-Molecule Observation of Dichloroaurate(I) Binding to an Engineered Mycobacterium smegmatis porin A (MspA) Nanopore.Analytical Chemistry,2021,93(3), 1529–1536. Ma, F. B.; Yan, S. H.; Zhang, J. Y.; Wang, Y.; Wang L. Y.; Wang Y. Q.; Zhang, S. Y.; Du, X. Y.; Zhang P. K.; Chen, H.-Y.; Huang, S.*, Nanopore Sequencing Accurately Identifies the Cisplatin Adduct on DNA.ACS Sensors,2021,6(8),3082–3092 Zhang, J. Y.; Cao, J.; Jia, W. D.; Zhang, S. Y.; Yan, S. H.; Wang, Y. Q.; Zhang, P. K.; Chen, H.-Y.; Li, W. F.*; Huang, S.*, Mapping potential engineering sites of Mycobacterium smegmatis porin A (MspA) to form a nanoreactor.ACS Sensors,2021, 6(6), 2449–2456 Wang, Y.#; Fan, P.#; Zhang, S.; Yan, S.; Huang, S.*, Preparation of Mycobacterium smegmatis porin A (MspA) nanopores for single molecule sensing of nucleic acids.Biophysics Reports,2021, 7(5): 355-364