Karatekin, Erdem - Yale University - Biological & Biomedical Sciences

个人简介

After obtaining his B.S. degree in chemical engineering from the University of Louisville, KY (where he studied thanks to a swimming scholarship) Dr. Karatekin went on to study soft matter physics and chemistry at Columbia University with N. J. Turro and B. O'Shaughnessy where he obtained his Ph.D. in 1999. He then gradually moved toward studying dynamics of lipid membranes, first during his post-doctoral stay at the Curie Institute (with F. Brochard-Wyart), and later as research faculty at the Laboratoire de Dynamique Membranaire (CNRS FRE 3146), both in Paris, France. Thanks to a long-term leave from the CNRS, he was a visiting research scientist in the laboratory of Dr. J. E. Rothman in Cell Biology at Yale during 2008-2011. He joined the Department of Cellular and Molecular Physiology as an assistant professor in 2012. PhD Columbia University, Chemistry (1999) MSc Columbia University (1996) BS University of Louisville, Chem. Eng. (1994) Visiting Research Scientist Yale University, School of Medicine Investigator Centre National de la Recherche Scientifique Post-doctoral fellow Curie Institute

研究领域

Exocytosis; Liposomes; Membrane Fusion; Microscopy, Fluorescence; Molecular Biology; Physiology; Secretory Vesicles; Microfluidics

近期论文

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SNARE-mediated fusion of single proteoliposomes with tethered supported bilayers in a microfluidic flow cell monitored by polarized TIRF microscopy J. Nikolaus*, E. Karatekin*, “SNARE-mediated fusion of single proteoliposomes with tethered supported bilayers in a microfluidic flow cell monitored by polarized TIRF microscopy”, J. Vis. Exp., e54349, doi:10.3791/54349 (2016). A Programmable DNA Origami Platform to Organize SNAREs for Membrane Fusion W. Xu, B. Nathwani, C. Lin, J. Wang, E. Karatekin, F. Pincet, W. Shih*, J. E. Rothman*, “A Programmable DNA Origami Platform to Organize SNAREs for Membrane Fusion”, J. Am.Chem. Soc., 138,4439-4447 (2016). Nanodisc-cell fusion: control of fusion pore nucleation and lifetimes by SNARE protein transmembrane domains. Z. Wu, S. M. Auclair, O. Bello, W. Vennekate, N. Dudzinski, S. Krishnakumar, and E. Karatekin*, “Nanodisc-cell fusion: control of fusion pore nucleation and lifetimes by SNARE protein transmembrane domains”, Sci. Rep., 6, 27287; doi: 10.1038/srep27287 (2016). Cholesterol Increases the Openness of SNARE-Mediated Flickering Fusion Pores. B. S. Stratton, Z. Wu, J. M. Warner, G. Wei, E. Karatekin*, and Ben O'Shaughnessy*, “Cholesterol Increases the Openness of SNARE-Mediated Flickering Fusion Pores” Biophys. J. 110, 1538–1550 (2016). Mechanism of Cytokinetic Contractile Ring Constriction in Fission Yeast. M. R. Stachowiak, C. Laplante, H. F. Chin, B. Guirao, E. Karatekin, T. D. Pollard, and Ben O'Shaughnessy*, “Mechanism of Cytokinetic Contractile Ring Constriction in Fission Yeast”, Dev. Cell, 29, 547–561, 2014. FisB mediates membrane fission during sporulation in Bacillus subtilis. T. Doan, J. Coleman, K. A. Marquis, B.M. Burton, E. Karatekin*, and D. Z. Rudner*, “FisB mediates the final membrane fission event during sporulation in Bacillus Subtilis”, Genes Dev., 27, 322-334, 2013. Fusion of single proteoliposomes with planar, cushioned bilayers in microfluidic flow cells. Karatekin E and Rothman JE. Fusion of single proteoliposomes with planar, cushioned bilayers in microfluidic flow cells. Nature Protocols, 7:903-920, 2012. Interactive, computer-assisted tracking of speckle trajectories in fluorescence microscopy: application to actin polymerization and membrane fusion. Smith MB, Karatekin E, Gohlke A, Mizuno H, Watanabe N, and Vavylonis D. Interactive, computer-assisted tracking of speckle trajectories in fluorescence microscopy: application to actin polymerization and membrane fusion. Biophys. J. 101:1794-1804, 2011. Coupling amperometry and total internal reflection fluorescence microscopy at ITO surfaces for monitoring exocytosis of single vesicles. Meunier A, Jouannot O, Fulcrand R, Fanget I, Bretou M, Karatekin E, Arbault S, Guille M, Darchen F, Lemaître F, and Amatore C. Coupling amperometry and total internal reflection fluorescence microscopy at ITO surfaces for monitoring exocytosis of single vesicles. Angew. Chem. Int. Ed., 50:5081-5084, 2011. A fast, single-vesicle fusion assay mimics physiological SNARE requirements. Karatekin E, Di Giovanni J, Iborra C, Coleman J, O'Shaughnessy B, Seagar M, and Rothman JE. A fast, single-vesicle fusion assay mimics physiological SNARE requirements. Proc. Natl. Acad. Sci. USA. 107:3517–3521, 2010. Model of SNARE-mediated membrane adhesion kinetics. Warner JM, Karatekin E, and O'Shaughnessy B. Model of SNARE-mediated membrane adhesion kinetics. PLoS One 4(8):e6375, 2009. A 20 nm step toward the cell membrane preceding exocytosis may correspond to docking of tethered granules. Karatekin E, Tran S, Huet S, Fanget I, Cribier S, and Henry JP. A 20 nm step toward the cell membrane preceding exocytosis may correspond to docking of tethered granules. Biophys. J. 94:2891-2905, 2008. Characterization of sequential exocytosis in a human neuroendocrine cell line using evanescent wave microscopy and `virtual trajectory' analysis.