Engelenburg, Schuyler van - University of Denver - Department of Biological Sciences

个人简介

2005 B.S., Chemistry – Fort Lewis College – Durango, CO 2010 Ph.D., Biochemistry – University of Colorado – Boulder, CO 2010-2014 NIH Postdoctoral Fellow, Cell Biology and Metabolism Program, NICHD – Bethesda, MD

研究领域

Host-cell factors involved in promoting virus assembly and release: Our laboratory is focused on understanding the mechanisms by which viral components usurp host cell machinery to carry out virus assembly and propagation. Specifically, we are interested in the ways in which enveloped viruses, such as Human Immunodeficiency Virus (HIV), hijack membrane remodeling components to mediate virus assembly and release. This research is enabled by the use of advanced fluorescence microscopy technologies such as three-dimensional point-localization superresolution imaging (PALM/STORM), which provides unparalleled cellular resolution and molecular specificity at < 50 nanometer spatial scales. Additionally, we use single-molecule FRET to probe the dynamics of key biomolecules at spatial scales < 10 nanometers. Combined these techniques allow us to spy on virus and host cell molecules in order to understand the structural principles that govern cellular and molecular function. Projects in the laboratory use a variety of supplementary techniques such as: mammalian cell culture, molecular biology, biochemistry, fluorescent probe/protein engineering, and image analysis to enable quantitative measurements of molecular and cellular systems. It is expected that these measurements will enable construction of accurate models of the host-pathogen interface with the eventual goal of therapeutic exploitation of viral dependencies.

近期论文

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Diverse protocols for correlative super-resolution fluorescence imaging and electron microscopy of chemically fixed samples. Kopek BG, Paez-Segala MG, Shtengel G, Sochacki KA, Sun MG, Wang Y, Xu CS, van Engelenburg SB, Taraska JW, Looger LL, Hess HF. Nat Protoc. 2017 May;12(5):916-946. doi: 10.1038/nprot.2017.017. Epub 2017 Apr 6. Expansion and concatenation of non-muscle myosin IIA filaments drive cellular contractile system formation during interphase and mitosis. Fenix AM, Taneja N, Buttler CA, Lewis J, Van Engelenburg SB, Ohi R, Burnette DT. Mol Biol Cell. 2016 Mar 9. pii: mbc.E15-10-0725. [Epub ahead of print] MicroRNA binding to the HIV-1 Gag protein inhibits Gag assembly and virus production. Chen AK, Sengupta P, Waki K, Van Engelenburg SB, Ochiya T, Ablan SD, Freed EO, Lippincott-Schwartz J. Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):E2676-83. doi: 10.1073/pnas.1408037111. Epub 2014 Jun 17. Correlative super-resolution fluorescence and metal-replica transmission electron microscopy. Sochacki KA, Shtengel G, van Engelenburg SB, Hess HF, Taraska JW. Nat Methods. 2014 Mar;11(3):305-8. doi: 10.1038/nmeth.2816. Epub 2014 Jan 26. Select item 24436186 Distribution of ESCRT machinery at HIV assembly sites reveals virus scaffolding of ESCRT subunits. Van Engelenburg SB, Shtengel G, Sengupta P, Waki K, Jarnik M, Ablan SD, Freed EO, Hess HF, Lippincott-Schwartz J. Science. 2014 Feb 7;343(6171):653-6. doi: 10.1126/science.1247786. Epub 2014 Jan 16. Superresolution imaging of biological systems using photoactivated localization microscopy. Sengupta P, van Engelenburg SB, Lippincott-Schwartz J. Chem Rev. 2014 Mar 26;114(6):3189-202. doi: 10.1021/cr400614m. Epub 2014 Jan 13. Review. No abstract available. Imaging type-III secretion reveals dynamics and spatial segregation of Salmonella effectors. Van Engelenburg SB, Palmer AE. Nat Methods. 2010 Apr;7(4):325-30. doi: 10.1038/nmeth.1437. Epub 2010 Mar 14. FACS-based selection of tandem tetracysteine peptides with improved ReAsH brightness in live cells. Van Engelenburg SB, Nahreini T, Palmer AE. Chembiochem. 2010 Mar 1;11(4):489-93. doi: 10.1002/cbic.200900689. No abstract available.