Qin, Yan - University of Denver - Department of Biological Sciences

个人简介

2000 B.S., Biology- Nanjing University- China 2002 M.S., Physiology- Nanjing University- China 2008 Ph.D., Neuroscience- Ohio University- Athens, Ohio 2009-2015, Postdoctoral research associate, Bio-imaging, University of Colorado at Boulder- Boulder, CO

研究领域

Zinc is an essential nutrient for biological function and human health. A pool of free zinc is concentrated in synaptic vesicles in glutamatergic neurons and co-released with glutamate during neuronal activity. The synaptically released zinc has been suggested to act a signaling molecule to communicate between cells and modulate various synaptic targets (receptors and ion channels) and synaptic plasticity, which would further modulate brain function. Zinc imbalance and dysregulation have been correlated with a number of human diseases. Currently, our understanding of zinc in neuronal signaling and neurodegeneration is limited by the lack of appropriate tools to specifically track the dynamics of zinc in living neurons, tissues and organisms. Therefore, the major objectives of my lab are to generate imaging systems to monitor zinc dynamics in living cells and organisms with high spatio-temporal fidelity, and to utilize these novel systems to investigate the biological functions of zinc. Our approach spans basic biophysical and biochemical technologies to molecular neuroscience techniques. We also incorporate considerable use of advanced microscope imaging and large imaging dataset analysis strategies in our research. We will investigate the zinc functions in ischemia/reperfusion by incorporating optimized zinc sensors into cultured neurons and zebrafish (with current collaborator Dr. Bruce Appel), as well as systematically study the cross-talk between zinc and calcium signaling.

近期论文

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Qin, Y, Sammond, D. W., Braselmann, E., Carpenter, M. C., & Palmer, A. E. Development of an Optical Zn2+ Probe Based on a Single Fluorescent Protein. ACS Chemical Biology. 2016 Qin Y, Miranda JG, Stoddard CI, Dean KM, Galati DF; Palmer AE. (2013) Direct comparison of a genetically encoded sensor and small molecule indicator: implications for quantification of cytosolic Zn2+. ACS Chemical Biology 8(11), 2366-2371. Link Miranda JG, Weaver AL, Qin Y, Park JG, Stoddard CI, et al. (2012) New Alternately Colored FRET Sensors for Simultaneous Monitoring of Zn2+ in Multiple Cellular Locations. PLOS ONE 7(11), e49371. Link Geng X, Huang C, Qin Y, McCombs J, Yuan Q, Harry BL, Palmer AE, Xia N, and Xue D. (2012). Hepatitis B virus X protein targets Bcl-2 proteins to increase intracellular calcium, required for virus replication and cell death induction. Proceedings of the National Academy of Sciences 109(45),18471-6. Link Park JG, Qin Y, Palmer AE. (2012). New sensors for quantitative measurement of mitochondrial Zn2+. ACS Chemical Biology 7(10),1636-40. Link Dean K, Qin Y, Palmer AE. (2012). Visualizing Metal Ions in Cells: An Overview of Analytical Techniques, Approaches, and Probes. Biochimica et Biophysica Acta 1823(9), 1406-15. Link West DC, Qin Y, Peterson QP, Thomas DL, Palchaudhuri R, Morrison KC, Lucas PW, Palmer AE, Fan TM, Hergenrother PJ. (2012). Differential Effects of Procaspase-3 Activating Compounds in the Induction of Cancer Cell Death. Molecular Pharmaceutics 9(5), 1425-34. Link Qin Y, Dittmer PJ, Park JG, Jansen KB, Palmer AE. (2011). Measuring steady-state and dynamic endoplasmic reticulum and Golgi Zn2+ with genetically encoded sensors. Proceedings of the National Academy of Sciences 108(18), 7351-6. Link Palmer AE, Qin Y, Park JG, McCombs JE. (2011). Design and application of genetically encoded biosensors. Trends in Biotechnology 29(3), 144-52. Link Qin Y, Thomas D, Fontaine CP, Colvin RA. (2009). Silencing of ZnT1 reduces Zn2+ efflux in Cultured Cortical Neurons. Neuroscience letters 450(2), 206-210. Link Qin Y, Thomas D, Fontaine CP, Colvin RA. (2008). Mechanisms of Zn2+ Efflux in Cultured Cortical Neurons. Journal of neurochemistry 107, 1304-1313. Link Qin Y, Gao J, Zhang TT, Zhao L, Wu HW, Xu L, Xu JY, Lu SY. (2002). Changes of thioredoxin mRNA level in neurons insulted by sodium azide, Shi Yan Sheng Wu Xue Bao 35(1), 26-30.