李刚 - 中山大学 - 生命科学学院

个人简介

教育经历： 1.1990/9-1994/6，山东大学，微生物学专业，学士。 2.1994/9-1997/6，中山大学，遗传学专业，硕士，导师：李宝健教授。 3.1997/9-2001/6, 中山大学，遗传学专业，博士，导师：李宝健教授。工作经历： 1.2001/7-2007/11 中山大学生命科学学院，讲师。 2.2007/12-至今，中山大学生命科学学院，副教授，博导；

研究领域

分子酶学与酶工程：利用宏基因组、宏转录组等手段从极端环境、肠道微生物中挖掘与木质纤维素降解相关的酶，并探索其独特酶学特性的分子机制，然后对其进行分子改造，并开展木质纤维素降解的应用研究；药用真菌的生物育种及发酵工程研究：利用紫外诱变、等离子诱变、太空诱变等手段对灵芝等药用真菌进行菌种诱变和选育，建立并优化其大规模工业化生产和后处理的工艺条件，并开展其中试和产业化研究

近期论文

查看导师新发文章（温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

1. Jia ML, Zhong XL, Lin ZW, Dong BX, Li G*. Expression and characterization of a new family of esterase isolated from a metagenomic library of paper mill sludge. Int J Biol Macromol (IF2017=3.909), 2018, accepted. 2. Gong BL, Mao RQ, Xiao Y, Jia ML, Zhong XL, Liu Y, Xu PL, Li G*. Improvement of enzyme activity and soluble expression of an alkaline protease isolated from oil-polluted mud flat metagenome by random mutagenesis. Enzyme Microb Technol, 2017,106:97-105. 3. Gao G, Mao RQ, Xiao Y, Zhou J, Liu YH, Li G*. Efficient yeast cell-surface display of an endoglucanase of Aspergillus flavuse and functional characterization of the whole-cell enzyme. World J Microbiol Biotechnol, 2017,33:114. 4. Xiao Y, Zhang QS, Luo YQ, Zhang Y, Luo X, Wang YC, Cao WG, de Pinto V, Liu QY, Li G*. Neurospora crassa tox-1 gene encodes a pH- and temperature-tolerant mini-cellulase. J Agr Food Chem （一区杂志,IF2017=3.412）, 2016, 64: 4751-4757. 5. Gao G, Wang An, Gong BL, Li QQ, Liu YH, He ZM, Li G*. A novel metagenome-derived gene cluster from termite hindgut: Encoding phosphotransferase system components and high glucose tolerant glucosidase. Enzyme Microb Technol, 2016,84:24-31. 6. Li L, Li G (并列第一), Cao LC, Ren GH, Kong W, Wang SD, Guo GS, Liu YH*. Characterization of the cross-linked enzyme aggregates of a novel β-galactosidase, a potential catalyst for the synthesis of galacto-oligosaccharides. J Agr Food Chem （一区杂志,IF2017=3.412）, 2015, 63: 894-901. 7. Zhang X, Li H, Li CJ, Ma T, Li G*, Liu YH. Metagenomic approach for the isolation of a thermostable b-galactosidase with high tolerance ofgalactose and glucose from oil samples of Turpan Basin. BMC Microbiol, 2013,13:237. 8. Li G, Dong BX, Liu YH, Li CJ, Zhang LP. Gene synthesis method based on overlap extension PCR and DNAWorks program. Method MolBiol, 2013, 1073:9-17. 9. Li CJ, Zhang X, Zhang LP, Wang A, Mao RQ, Li G*. Medium optimization for the production of a metagenome-derived b-galactosidase by Pichia pastoris using response surface methodology. Afr J Microbiol Res, 2013,7:1077-1085. 10. Li G, Jiang Y, Fan XJ, Liu YH. Molecular cloning and characterization of a novel β-glucosidase with high hydrolyzing ability for soybean isoflavone glycosides and glucose-tolerance from soil metagenomic library. Bioresource Technol （一区杂志，IF2017=5.807）, 2012, 123:15-22. 11. Sang SL, Li G, Hu XP, Liu YH. Molecular cloning, overexpression and characterization of a novel feruloyl esterase from a soil metagenomic library. J Mol Biol Biotechnol, 2011, 20:196-203. 12. Liu YH, Ye M, Lu Y, Zhang X, Li G*. Improving the decolorization for textile dyes of a metagenome-derived alkaline laccase by directed evolution, Appl Microbiol Biotechnol, 2011,91:667-675. 13. Wang K, Li G(并列第一), Yu SQ, Zhang CT, Liu YH. A novel metagenome-derived β-galactosidase: gene cloning, overexpression,purification and characterization, Appl Microbiol Biotechnol, 2010,88:155-165. 14. Ye M, Li G(并列第一), Liang WQ, Liu YH. Molecular cloning and characterization of a novel multicopper oxidase with laccase activity originating from mangrove soil via metagenomic approach. Appl Microbiol Biotechnol, 2010,87:1023-1031. 15. Li G, Wang K，Liu YH. Molecular cloning and characterization of a novel pyrethroid-hydrolyzing esterase originating from the Metagenome. Microb Cell Fact （IF=3.831，二区杂志）, 2008,7:38. 16. Dong BX, Mao RQ, Li BJ, Liu QY, Xu PL, Li G*. An improved method of gene synthesis based on DNAWorks software and overlap extension PCR. Mol Biotechnol, 2007,37:195-200. 17. Dong BX, Ouyang L, Qin L, Li G*. A rapid and simple method for screening large numbers of recombinant DNA clones. J Rapid Meth Aut Microbiol, 2007, 15:244-252. 18. Wang ZF, Li G*, Fu SL, Ren H. Isolation and characterization of microsatellite loci in Cryptocarya chinensis in lower subtropical China,Conservation Genetics, 2007, 8:1235-1237. 19. Li G, Li RX, Liu QY, Wang Q, Chen M, Li BJ. A Highly efficient Polyethylene Glycol-mediated transformation method for mushrooms, FEMS Microbiol Lett, 2006,256:203-208.